Project description:Vibrio ordalii is the causative agent of atypical vibriosis in salmonids cultured in Chile. While extensive research has provided valuable insights into V. ordalii through phenotypic, antigenic, and genetic typing, as well as various virulence mechanisms (e.g., capsular material, iron-uptake systems, and outer membrane vesicles [OMVs]), proteomic characterization remains largely unexplored. This study aimed to advance the proteomic knowledge of Chilean V. ordalii Vo-LM-18 and its OMVs, which have been extensively characterized in several studies and have demonstrated their virulence. Using Nano-UHPLC-LC-MS/MS, we identified 2,242 proteins in the bacterium and 1,755 proteins in its OMVs. Among these, 644 unique proteins were detected in V. ordalii Vo-LM-18, 156 unique proteins were found in its OMVs, and 1,596 proteins were shared between both components. bioinformatics compartment prediction (PSORT algorithm) revealed that the major categories in the OMVs were similar to those in the bacteria (i.e., cytoplasmic and cytoplasmic membrane proteins), though with different proportions. Functional annotation with Gene Ontology identified 37 biological pathways in V. ordalii Vo-LM-18 and 28 in its OMVs. Proteins associated with transport, transcription, and virulence were predominant in both the bacteria and the vesicles. Comparison between the strain Vo-LM-18 and its OMVs showed evident differences in protein expression, with OMVs expressing a higher number of virulence-associated proteins, including those related to iron- and heme-uptake mechanisms. Notable pathways in the bacteria included flagellum assembly, heme group-associated proteins, and protein biosynthesis. This proteomic analysis also represents the first detection of RTX toxin in a V. ordalii strain (Vo-LM-18) and its vesicles. Our results highlight the crucial role of OMVs in the pathogenesis and adaptation of V. ordalii, suggesting their potential as diagnostic biomarkers and therapeutic targets for bacterial infections.
Project description:Bacterial strains within a species can differ in traits that shape host–microbe interactions. Using the squid–vibrio symbiosis, we examined how strain-level variation influences host responses. Strains from squid and fish light organs were compared for their effects on Euprymna scolopes gene expression and development. Non-native strains showed reduced colonization success and delayed host phenotypes. Transcriptomic responses were most similar with native isolates and most divergent with a fish-derived strain. These findings demonstrate that fine-scale strain differences shape host compatibility and underscore the importance of strain-level diversity in the establishment of beneficial symbioses.
Project description:Investigation of whole genome gene expression level changes in a Vibrio cholerae O395N1 delta-nqrA-F mutant, compared to the wild-type strain.
Project description:Environmental isolates of Vibrio cholerae from California coastal water compared to reference strain N16961. A genotyping experiment design type classifies an individual or group of individuals on the basis of alleles, haplotypes, SNP's. Keywords: genotyping_design; array CGH
