Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

HybriSeq: Probe-based Device-free Single-cell RNA Profiling

ABSTRACT: HybriSeq: Probe-based Device-free Single-cell RNA Profiling

PROVIDER: PRJNA1237907 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

HybriSeq: Probe-based Device-free Single-cell RNA Profiling

Project description:We have developed the HybriSeq method for single-cell RNA profiling, which utilizes in situ hybridization of multiple probes for targeted transcripts, followed by split-pool barcoding and sequencing analysis of the probes. We have shown that HybriSeq can achieve high sensitivity for RNA detection with multiple probes and profile entire transcripts without an end bias. The utility of HybriSeq is demonstrated in characterizing cell-to-cell heterogeneities of a panel of 196 genes in peripheral blood mononuclear cells and the detection of missed annotations of transcripts.

2025-08-02 | GSE292322 | GEO

A diffusion-based microfluidic device for single-cell RNA-seq

Project description:Microfluidic devices provide a low-input and efficient platform for single-cell RNA-seq (scRNA-Seq). Here we present microfluidic diffusion-based RNA-seq (MID-RNA-seq) for conducting scRNA-seq with a diffusion-based reagent swapping scheme. This device incorporates cell trapping, lysis, reverse transcription and PCR amplification all in one microfluidic chamber. MID-RNA-Seq provides high data quality that is comparable to existing scRNA-seq methods while implementing a simple device design that permits multiplexing. The robustness and scalability of MID-RNA-Seq device will be important for transcriptomic studies of scarce cell samples.

2019-02-25 | GSE119271 | GEO

A diffusion-based microfluidic device for single-cell RNA-seq

Project description:A diffusion-based microfluidic device for single-cell RNA-seq

| PRJNA488637 | ENA

probe based bacterial single-cell RNA sequencing predicts toxin regulation

Project description:probe based bacterial single-cell RNA sequencing predicts toxin regulation

| PRJNA928315 | ENA

Probe-Seq enables transcriptional profiling of specific cell types from heterogeneous tissue by RNA-based isolation

Project description:Probe-Seq is a method that allows transcriptional profiling of specific cell types from heterogeneous tissue by labeling RNA. Briefly, we developed Probe-Seq, which allows deep transcriptional profiling of specific cell types isolated based on RNA abundance of defined markers. We applied Probe-Seq to purify and profile specific cell types from mouse, human, and chick retinas as well as the Drosophila gut. We also showed that Probe-Seq is compatible with frozen nuclei and that multiplexed Probe-Seq enables iterative isolation of multiple cell types from the same tissue sample. Provided that unique markers are available, this simple, novel method could potentially enable bulk RNA sequencing of any cell type from any organism.

2019-12-09 | GSE135572 | GEO

Device-free isolation of photoreceptor cells from patient iPSC-derived retinal organoids

Project description:Device-free isolation of photoreceptor cells from patient iPSC-derived retinal organoids

| PRJNA1107509 | ENA

Rapid purification and multiparametric characterization of circulating small extracellular vesicles utilizing a label-free lab-on-a-chip device

Project description:Exosomes were isolated from various biofluids from cancer patients using a novel device and sequenced to validate the device.

2023-09-12 | E-MTAB-13288 | biostudies-arrayexpress

Label-free melanoma phenotype classification using AI-based morphological profiling

Project description:Label-free melanoma phenotype classification using AI-based morphological profiling

| PRJNA1140860 | ENA

microRNA-seq in circCDYL probe and NC probe

Project description:microRNA-seq in circCDYL probe and NC probe