Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1241471 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:This study examines whether maternal low ω6:ω3 ratio diet and offspring seaweed (SW) supplementation can improve offspring immunity and performance by elucidating the effects on piglet serum proteome. A total of 16 sows were given either a standard (CR, 13:1) or low ω6:ω3 ratio diet (LR, 4:1) during pregnancy and lactation and their male weaned piglets were supplemented with SW powder (4 g/kg, SW) or not (CT) in a 21-day post-weaning (PW) diet. Four PW piglet groups were then identified based on dam and piglet treatment, namely CRCT, CRSW, LRCT, and LRSW (n = 10 each). Piglet serum collected at weaning and d21 PW were analyzed (n = 5 each) using TMT-based quantitative proteomics and validated by appropriate assays.
2023-04-17 | PXD032327 | Pride
Project description:Microbiome analysis has relied largely on metagenomics to characterize microbial populations and predict their functions. Here, we used a TMT LC-MSMS metaproteomic analysis of the fecal microbiome in piglets before and after weaning to compare protein abundances as they pertain to microbial populations specific to either a milk- or plant-based diet. Fecal samples were collected from six piglets on the day of weaning and four weeks after transitioning to a standard nursery diet. Using the 12,554 protein groups identified in samples, we confirmed the shift in protein composition that takes place in response to the microbial succession following weaning and demonstrated the redundancy in metabolic processes between taxa. We identified taxa with roles as primary degraders based on corresponding proteins synthesized, thereby providing evidence for cross-feeding. Proteins associated with the breakdown of milk-specific carbohydrates were common among pre-weaned pigs, whereas the proteome of post-weaned piglets contained a greater abundance of proteins involved in the breaking down plant-specific carbohydrates. Furthermore, output revealed that production of propionate takes place via the propionaldehyde pathway in pre-weaned piglets, but changes to production via the succinate pathway in post-weaned piglets. Finally, a disproportionate quantity of carbohydrate-active enzymes (CAZymes) (~8%) were produced by fungi, which typically only represent ~0.1% of the microbiome taxa. Information gathered through this characterization of the metaproteome before and after weaning revealed important differences regarding the role of members in the microbial community, thereby providing information for the optimization of diets and products for both piglet and microbiome health.
2025-05-26 | PXD063155 | Pride
Project description:probiotics and prebiotics to maternal diets is related to decreased incidence of diarrhea and greater weight gain during lactation. Our objective was to determine the impact of adding whole ground oat as a prebiotic alone or in combination with postbiotic yeast culture (YC) (Saccharomyces cerevisiae) to sow gestation and lactation rations on milk composition, piglet growth, and incidence of post weaning diarrhea (PWD). Diets: control (CON), CON + yeast culture (YC) [5g/kg], CON + oat (15% inclusion rate) (Oat) or CON+ YC [5g/kg] + Oat (15%) were fed during the last 30 days of gestation and throughout lactation (18-21 days). Shotgun proteome analysis of day 4 and 7 postpartum milk samples found 36 differentially abundant proteins (P-adj <0.1) in both Oat and YC supplemented sows relative to CON. Notable was increased expression of antimicrobial proteins, lactoferrin and chitinase. IgG in milk of Oat supplemented sows was lower than YC supplemented sows (p<0.05) but had greater E. coli-antigen reactivity. Piglet weights at birth were similar. At weaning YC + Oat piglets weighed less and gained less weight (p<0.05) postweaning than CON. The incidence of PWD was lowest in the YC and Oat groups compared to CON and YC+ Oat groups. These data suggest that Oat or YC culture supplementation alters milk immune and antimicrobial associated proteins that can impact piglets but may have negative effects on piglet growth when given in combination.
2020-12-05 | MSV000086562 | MassIVE
Project description:We set out to better understand the cellular heterogeneity and niche cues of the remodeling porcine intestine after weaning by performing single-cell mRNA sequencing (scRNA-seq) survey of 42,149 cells from ileum tissue in piglets. Substantial heterogeneity among diverse epithelial, stromal, and immune cell subsets was observed. We map the various cell committed state within ileum tissue, and connect them through cell-cell interactions. We delineated a new classification of different epithelium secretory cells. Apart from goblet cell and enteroendocrine cell, we found that few pH-sensing BEST4/OTOP2 cells also exist in the ileum13. The immune component of early-weaned piglet was shifted as reduction of Th17 and abundance of CTL. Th17 cells harbor features of classical phonotype in normal suckling piglet (NSP) and pathogenicity in early weaning piglet (EWP), which may be induced by special cytokine milieu suggested as previous stduy14,15. We conclusively showed enrichment of the pathogenic effector CD8+ GZMB+ CTL cells may be a critical factor exacerbating ileal inflammation via mitochondrial dysfunction inducing epithelia cell apoptosis in EWP. Keywords: Expression profiling by high throughput sequencing
2025-12-11 | GSE174112 | GEO
Project description:Weaning piglet colonic microbes Raw sequence reads
| PRJNA1037299 | ENA
Project description:Early weaning commonly results in gastrointestinal disorders, inflammation and diarrhea in infants and young animals. Resveratrol, a plant phenol, affords protection against inflammation and cancer.A porcine model was used to investigate the effects of maternal resveratrol supplementation on diarrhea, intestinal inflammation and intestinal morphology in offspring during weaning. The intestinal gene expression was measureed by RNA sequencing (RNA-seq) analysis. Results that weaning-associated intestinal inflammation and diarrhea in pig offspring were alleviated and intestinal morphology was improved by maternal resveratrol supplementation. In weaning piglets (21-day-old), RNA-seq showed that differentially expressed genes (DEGs) were enriched for T cell receptor, primary immunodeficiency, mitogen-activated protein kinase (MAPK) and Ras signaling pathway. In post-weaning piglets (28-day-old), RNA-seq showed that DEGs were enriched in the cytokine-cytokine receptor interaction pathway and pathways related to metabolism. This study provided insight into molecular mechanisms underlying the effects of maternal dietary resveratrol.
2020-04-30 | GSE107474 | GEO
Project description:In this study, differentially prepared Arnica extracts and the lead compounds Thymol and Helenalin were directly compared for their immunomodulatory potential using primary human T cells. Extracts from different plant parts (root or whole plant) and prepared by different manufacturing processes inhibited T cell activation and proliferation. nCounter gene expression analysis and subsequent gene set enrichment analysis showed that Arnica preparations intervene in T cell receptor (TCR) signaling. Interestingly, the three tested extracts were, however, found to mediate their effects via interfering with different TCR signaling pathways. The hydroethanolic root extract selectively inhibited the binding of NFκB to DNA, while the aqueous fermented whole plant extract preferentially inhibited NFAT-dependent gene expression. The hydroethanolic whole plant extract (by trend) diminished both, NFκB DNA-binding and NFAT-dependent gene expression.
2025-10-15 | GSE299224 | GEO