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Gene expression profiling of human umbilical vein endothelial cells overexpressing CELF2 as diagnostic targets in diabetes-induced erectile dysfunction

ABSTRACT: Gene expression profiling of human umbilical vein endothelial cells overexpressing CELF2 as diagnostic targets in diabetes-induced erectile dysfunction

PROVIDER: PRJNA1242836 | ENA |

REPOSITORIES: ENA

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Gene expression profiling of human umbilical vein endothelial cells overexpressing CELF2 as diagnostic targets in diabetes-induced erectile dysfunction

Project description:Background: Erectile dysfunction (ED) is a common complication of diabetes mellitus (DM), and because of its complex neurovascular etiology, the associated molecular pathogenic mechanisms are not fully understood. This study investigated the important functions and potential molecular regulatory roles of CELF2 in DMED. Methods: An in vitro HUVEC model with CELF2 overexpression was successfully established via transfection with a CELF2-overexpressing lentiviral vector. The effects of CELF2 overexpression on cell proliferation and angiogenesis were assessed via CCK-8 and angiogenesis assays. RNA sequencing was employed to evaluate the gene expression profiles and alternative splicing events regulated by CELF2. An RNA-sequencing assay was performed to evaluate gene expression profiles and alternative splicing genes in HUVECs overexpressing CELF2, and an integration analysis was combined with GSE146078 data to detect potential target genes related to DMED. Results: The expression of genes related to angiogenesis and the immune response significantly increased with CELF2 overexpression, and the four hub genes associated with alternative splicing in aging and angiogenesis were CXCL2, CXCL10, IL-1A and IL-6. Conclusions: CELF2 appears to be a key factor in DMED, influencing gene expression and alternative splicing related to angiogenesis and immune responses. The identified hub genes (CXCL2, CXCL10, IL-1A, and IL-6) are closely related to DMED and warrant further investigation to understand the underlying mechanisms and potential therapeutic implications. Keywords: Diabetes mellitus; Erectile dysfunction; RBPs; CELF2; RNA sequencing

2025-07-30 | GSE293115 | GEO

HUVEC Gene Expression of Irradiated Human Umbilical Vein Endothelial Cells

Project description:Ionizing radiation has become common for diagnostic and therapeutic purpose. Since it affects the cells at genetic level, it becomes necessary to evaluate its affects especially on endothelial cells which comprises the blood vessels and thus contributes in migration of blood cells, nutrients, oxygen etc. Human Umbilical Vein Endothelial Cells (HUVECs) are major model system used in vitro to study the nature and behaviour of endothelial cells.

2020-09-30 | GSE108723 | GEO

MicroRNA expression profile of human umbilical vein endothelial cells treated with Angiogenin (ANG)

Project description:We quantified differential microRNA (miRNA) expression in Human umbilical vein endothelial cells （HUVECs）response to Angiogenin (ANG) treatment.These data were used to determine which miRNAs are altered on ANG in Human umbilical vein endothelial cells.

2016-12-13 | GSE92256 | GEO

Application research of recanalization of the umbilical vein in portal vein embolization in radical hepatectomy of colorectal liver metastasis

Project description:Interventions: One group:portal vein embolization approach-recanalization of the umbilical vein Primary outcome(s): Portal vein catheter success rate Study Design: historical control

| 2668806 | ecrin-mdr-crc

Oxidoreductase transcript data from human umbilical vein endothelial cells

Project description:Oxidoreductase enzymes are critical to redox regulation of intracellular proteins within human cells. We used microarrays to identify which oxidreducatse genes are expressed in unstimulated human umbilical vein endothelial cells. Human umbilical vein endothelial cells were grown under optimal conditions and then RNA extracted and hybridized on Affymetrix microarrays.

2010-06-16 | E-GEOD-22166 | biostudies-arrayexpress

RNA-seq of CYR61-treated human umbilical vein endothelial cells

Project description:We assess the effect of CYR61 treatment on the transcriptome of human umbilical vein endothelial cells.

2023-10-18 | GSE222101 | GEO

Effect of from Human Umbilical Vein Endothelial Cells

Project description:We profiled global gene expression in primary human umbilical vein endothelial cells to determine the gene expression changes associated with knocking down PKM2 and p53. We identified a p53 dependent transcriptional response that remodels metabolism in cells lacking p53, thus limiting cell growth. Human Umbilical Vein Endothelial Cells were transfected with siRNA duplexes targeting PKM2 and / or p53, RNA was extracted and subjected to RNA sequencing

2016-07-27 | E-GEOD-84877 | biostudies-arrayexpress

Secretome analysis of Human Umbilical Vein Endothelial Cells with PMA treatment using gel-LC-MS/MS

Project description:Secretome analysis of Human Umbilical Vein Endothelial Cells with PMA treatment using gel-LC-MS/MS

2013-09-09 | PRD000733 | Pride

Oxidoreductase transcript data from human umbilical vein endothelial cells

2010-06-05 | GSE22166 | GEO

ChIP-seq from endothelial cell of umbilical vein (ENCSR000ALG)

Project description:Control ChIP-seq on human endothelial cell of umbilical vein For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2017-03-09 | GSE95866 | GEO

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