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Stage-specific requirement for Mettl3-dependent m6A epitranscriptomic regulation during myogenesis [RNA-Seq]

ABSTRACT: Stage-specific requirement for Mettl3-dependent m6A epitranscriptomic regulation during myogenesis [RNA-Seq]

PROVIDER: PRJNA1243358 | ENA |

REPOSITORIES: ENA

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Stage-specific requirement for Mettl3-dependent m6A epitranscriptomic regulation during myogenesis [meRIP-Seq]

Project description:The role of N6-methyladenosine (m6A) in muscle formation and maintenance remains incompletely understood, particularly with respect to the contribution of methyltransferase-like 3 (METTL3), the core component of the methyltransferase complex (MTC), to myogenesis. Here, we show the involvement of METTL3 in the m6A epitranscriptome during myogenesis. Our findings reveal a correlation between METTL3-regulated m6A modifications and myoblast fusion during differentiation and regeneration. We observed significant upregulation of METTL3 following injury and identified numerous alterations in myogenic pathways. Furthermore, we detected differential patterns of METTL3-regulated m6A modifications in genes during differentiation, with some genes exhibiting loss and others showing gain. Our study revealed that the fusion factors Mymx and Mymk are downstream elements of METTL3 involved in myogenesis, and both exhibit upregulated expression and m6A levels during myogenesis. Our findings provide valuable resources for elucidating the function and mechanism of METTL3-regulated m6A modifications in myogenesis, thereby revealing the regulatory role of METTL3 in myoblast fusion.

2025-09-03 | GSE293212 | GEO

Stage-specific requirement for Mettl3-dependent m6A epitranscriptomic regulation during myogenesis [RNA-Seq]

2025-09-03 | GSE293208 | GEO

Stage-specific requirement for Mettl3-dependent m6A epitranscriptomic regulation during myogenesis [meRIP-Seq]

Project description:Stage-specific requirement for Mettl3-dependent m6A epitranscriptomic regulation during myogenesis [meRIP-Seq]

| PRJNA1243359 | ENA

Stage-specific requirement for Mettl3-dependent m6A mRNA methylation during hematopoietic stem cell differentiation

Project description:Stage-specific requirement for Mettl3-dependent m6A mRNA methylation during hematopoietic stem cell differentiation

| PRJNA509123 | ENA

METTL3 shapes m6A epitranscriptomic landscape for successful human placentation.

Project description:Methyltransferase-like 3 (METTL3), the catalytic enzyme of methyltransferase complex for m6A methylation of RNA, is essential for mammalian development. However, the importance of METTL3 in human placentation remains largely unexplored. Here, we show that a fine balance of METTL3 function in trophoblast cells is essential for successful human placentation. Both loss-of and gain-in METTL3 functions are associated with adverse human pregnancies. A subset of recurrent pregnancy losses and preterm pregnancies are often associated with loss of METTL3 expression in trophoblast progenitors. In contrast, METTL3 is induced in pregnancies associated with fetal growth restriction (FGR). Our loss of function analyses showed that METTL3 is essential for the maintenance of human TSC self-renewal and their differentiation to extravillous trophoblast cells (EVTs). In contrast, loss of METTL3 in human TSCs promotes syncytiotrophoblast (STB) development. Global analyses of RNA m6A modification and METTL3-RNA interaction in human TSCs showed that METTL3 regulates m6A modifications on the mRNA molecules of critical trophoblast regulators, including GATA2, GATA3, TEAD1, TEAD4, WWTR1, YAP1, TFAP2C and ASCL2, and loss of METTL3 leads to depletion of mRNA molecules of these critical regulators. Importantly, conditional deletion of Mettl3 in trophoblast progenitors of an early post-implantation mouse embryo also leads to arrested self-renewal. Hence, our findings indicate that METLL3 is a conserved epitranscriptomic governor in trophoblast progenitors and ensures successful placentation by regulating their self-renewal and dictating their differentiation fate.

| S-EPMC11257629 | biostudies-literature

m6A-Epitranscriptomic Microarray analysis of CD11b+Mac-Spleen from HFD-Mettl3-LysM-Cre mice

Project description:We processed m6A-Epitranscriptomic Microarray analysis on splenic CD11b+ macrophages isolated from 10-week old Mettl3f/f-LysM-Cre KO and littermate WT mice.

2022-01-07 | GSE184367 | GEO

m6A-Epitranscriptomic Microarray analysis of CD11b+Mac-Spleen from HFD-Mettl3-LysM-Cre mice

Project description:m6A-Epitranscriptomic Microarray analysis of CD11b+Mac-Spleen from HFD-Mettl3-LysM-Cre mice

| PRJNA764228 | ENA

Depletion of m6A methylation by conditional Mettl3 knockout in Ascl1-induced neurons

Project description:We have perturbed RNA methylation machinery and investigated the change in subcellular localization of mRNA in Ascl1-induced neurons (iNeurons). Mutant iNeuron line harbouring tagged endogenous Mettl3 was generated which allowed for the selective and targeted depletion of Mettl3 protein with the addition of dTAG. Cells were treated with dTAG for 40 hours then separated into compartments (neurites and soma) and then sequenced in parallel with samples which received no dTAG treatment.

2023-07-12 | E-MTAB-9643 | biostudies-arrayexpress

m6A-mRNA&lncRNA Epitranscriptomic Microarray

Project description:Total RNA samples from human bronchial epithelial BEAS-2B passage-matched control cells and Cr(VI)-transofmred BEAS-2B cells were submitted to ArraySatr for total RNA m6A epitranscriptomic microarray analysis

2022-06-29 | GSE186605 | GEO

Murine kidneys： WT Models vs METTL3-cKO Models

Project description:MeRIP and RNA Epitranscriptomic Microarray analysis in kidneys of conditional knockout (cKO) METTL3 mice and wild type mice following glyoxylate-induced CaOx nephrocalcinosis.In METTL3-cKO mice, METTL3 subjected the specific absence from renal tubular epithelial cells. Goal was to determine the effects of METTL3 gene knockout on the expression and N6-methyladenosine (m6A) modifications of global gene.

2025-09-05 | GSE212868 | GEO

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