Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1248050 | ENA |
REPOSITORIES: ENA
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Project description:Appropriate soil nutrient management is critical for modern Canola (Brassica napus L.) varieties and hybrids to meet their yield potentials. Canola fields are typically supplemented by the application of nitrogen, phosphorus, sulphur, and to a lesser extent, potassium fertilizers to maximize yield, while deficiency in these key macronutrients can result in severe growth phenotypes and significant yield losses. To date, our understanding of canola nutrient deficiency responses is disparate, encompassing a large number of varieties using a variety of technologies and assessment criteria, with seminal understanding of the overlaps between nutrient deficiencies responses, having not yet been defined, placing limitations on our ability to increase the nutrient efficiency of this critical oil seed. To address this, we performed a comparative quantitative proteomics analysis of both shoot and root tissue harvested from soil-grown Canola plants experiencing either nitrogen, phosphorus, potassium, or sulphur deficiency. Our results show intriguing similarities in plant responses to deficiency in multiple nutrients. We also find very distinct proteome-level changes between shoot and root tissue of plants experiencing nutrient stress, suggesting the presence of highly organ-specific responses to nutrient deficiency. Our results pave the way for a more comprehensive understanding of the shared and distinct response mechanisms of plants to multiple essential nutrients.
2025-05-06 | PXD032223 | Pride
Project description:Phosphorus (P) is an essential macronutrient for various biological processes in plant growth. Modern agricultural science has advanced the knowledge of regulatory mechanisms underlying phosphorus starvation responses (PSRs), aiming to develop phosphate-efficient crops with sustainable production under reduced Pi fertilizer application. However, information regarding coordinated shoot and root adaptation in response to combined nutrient stresses is limited. This study investigated the role of Phloem Phosphate Stress Repressed 1 (PPSR1) in modulating PSRs and other nutrient adaptation. The Arabidopsis functional homologue of Cucumis sativus PPSR1 (CsPPSR1), designated AtPPSR1, was identified. AtPPSR1 encodes a glycine-rich domain-containing protein, and its ectopic expression confers enhanced growth performance to plants. Transcriptomic analyses revealed AtPPSR1 as a regulatory mediator of PSRs, photosynthesis and root development. We revealed that AtPPSR1 interacted with PHOSPHATE STARVATION RESPONSE 1 (PHR1) to regulate PHR1-target genes for adaptive root development, in response to Pi-starvation stress. Additionally, AtPPSR1 was discovered as graft-transmissible, and the shoot-borne AtPPSR1 played a role in restoring the root phenotype of the ppsr1 mutant. Physiological analyses revealed that enhanced AtPPSR1 expression enabled resilience to nitrogen (N) and potassium (K)-starvation, as well as to Pi-deficiency. Furthermore, we identified homologues of CsPPSR1 and AtPPSR1 in Brassica napus (canola), which displayed similar expression patterns in response to Pi-starvation stress. Overexpression of PPSR1, identified from Arabidopsis, cucumber and canola, improved growth performance and seed yield in canola under N-, Pi- or K-deficient conditions. These findings provide novel insights into PPSR1-mediated molecular coordination to improve growth performance under mineral nutrient-stresses conditions.
2025-12-13 | GSE292879 | GEO
Project description:Clubroot of Brassicaceae, an economically important soil borne disease, is caused by Plasmodiophora brassicae Woronin, an obligate, biotrophic protist. This disease poses a serious threat to canola and related crops in Canada and around the globe causing significant loss to seed yield. The pathogen is continuously evolving and new pathotypes are emerging, this necessitates the development of novel resistant canola cultivars to manage the disease effectively. Given that proteins play a crucial role in majority of biological processes and molecular functions, the identification of differentially abundant proteins (DAP) using proteomics information is an attractive approach to understand the plant-pathogen interactions as well as in the future development of gene specific markers for developing clubroot resistant (CR) cultivars. In this study, P. brassicae pathotype 3 (P3H) was used to challenge CR and clubroot susceptible (CS) canola lines. Root samples were collected at three distinct stages of pathogenesis, 7-, 14-, and 21-days post inoculation (DPI), protein samples were isolated, digested with trypsin and subjected to LC-MS/MS analysis. A total of 937 proteins demonstrated a significant (q < 0.05) change in abundance in at least in one of the time points when compared between control and inoculated CR-parent, CR-progeny, CS-parent, CS-progeny and 784 proteins were significantly (q < 0.05) changed in abundance in at least in one of the time points when compared between the inoculated- CR and CS root proteomes of parent and progeny across the three time points tested. Functional annotation of the differentially abundant proteins (DAPs) revealed several proteins related to calcium dependent signaling pathways in response to the pathogen. In addition, proteins related to reactive oxygen species (ROS) biochemistry, dehydrins, lignin, thaumatin, and phytohormones were identified. Among the DAPs, 74 putative proteins orthologous to CR proteins and quantitative trait loci (QTL) associated with eight CR loci in four chromosomes including chromosomes A3 and A8 were identified. In conclusion, these results have contributed to an improved understanding of the mechanisms that are involved in mediating response to P. brassicae in canola at the protein level.
2022-03-22 | PXD031070 | Pride
Project description:This study evaluated the transcriptomic profiles of Arabidopsis thaliana (Col-0) plants grown along bacterial isolates whose interactions induce root morhology changes in the plant
2019-05-15 | GSE131157 | GEO
Project description:Differences in root development in winter and spring canola
| PRJNA490162 | ENA