Homo sapiens
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ABSTRACT:
PROVIDER: PRJNA125147 | ENA |
REPOSITORIES: ENA
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Project description:Induced pluripotent stem (iPS) cells have been generated from mouse and human somatic cells by ectopic expression of the transcription factors OCT4, SOX2, KLF4, c-MYC as well as NANOG and LIN28. Here we report generation of induced pluripotent stem cells from human umbilical cord blood derived unrestricted somatic stem cells (USSC) using retroviral expression of the transcription factors OCT4, SOX2, KLF4 and C-MYC and evaluation of their molecular signature and differentiation potential in comparison to human embryonic stem cells. The reprogrammed cells (HUiPS) were analysed morphologically, by qRT-PCR, global miRNA and epigenetic profiling and gene expression microarrays, as well as in their in vitro and in vivo differentiation potential by embryoid body formation and teratoma assay. The cord blood iPS cells are highly similar to human embryonic stem cells morphologically, at their molecular signature as well as in their in vitro and in vivo differentiation potential. Human cord blood derived unrestricted somatic stem cells offer an attractive source of cells for the generation of induced pluripotent stem cells. Our findings open novel perspectives to generate HLA matched pluripotent stem cell banks based on existing cord blood banks. Besides its obvious relevance of such a second generation cord blood iPS bank for pharmacological and toxicological testing, its application for autologous or allogenic regenerative cell transplantation appears feasible. For transcriptome profiling, 400 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 10h). Quality-checked cRNA samples were hybridized as biological or technical duplicates for 18 h onto HumanRef-8 v3 expression BeadChips (Illumina), washed, stained, and scanned following guidelines and using materials / instrumentation supplied / suggested by the manufacturer. Six sample types were analyzed, each one of them in duplicate. USSC: human umbilical cord blood unrestricted somatic stem cells (duplicates) HUiPS: human iPS cells from human umbilical cord blood USSC, hand-picked cols (duplicates) H9 hESC: H9 human ESCs grown on low-density CF1 MEFs (duplicates) H1 hESC: H1 human ESCs grown on low-density CF1 MEFs (duplicates) 1F hNiPS: One factor (Oct4) human iPS cells from hNSCs, hand-picked cols (duplicates) 2F hNiPS: Two factors (Oct4, Klf4) human iPS cells from hNSCs, hand-picked cols (duplicates)
2010-07-07 | E-GEOD-20532 | biostudies-arrayexpress
Project description:Induced pluripotent stem (iPS) cells have been generated from mouse and human somatic cells by ectopic expression of the transcription factors OCT4, SOX2, KLF4, c-MYC as well as NANOG and LIN28. Here we report generation of induced pluripotent stem cells from human umbilical cord blood derived unrestricted somatic stem cells (USSC) using retroviral expression of the transcription factors OCT4, SOX2, KLF4 and C-MYC and evaluation of their molecular signature and differentiation potential in comparison to human embryonic stem cells. The reprogrammed cells (HUiPS) were analysed morphologically, by qRT-PCR, global miRNA and epigenetic profiling and gene expression microarrays, as well as in their in vitro and in vivo differentiation potential by embryoid body formation and teratoma assay. The cord blood iPS cells are highly similar to human embryonic stem cells morphologically, at their molecular signature as well as in their in vitro and in vivo differentiation potential. Human cord blood derived unrestricted somatic stem cells offer an attractive source of cells for the generation of induced pluripotent stem cells. Our findings open novel perspectives to generate HLA matched pluripotent stem cell banks based on existing cord blood banks. Besides its obvious relevance of such a second generation cord blood iPS bank for pharmacological and toxicological testing, its application for autologous or allogenic regenerative cell transplantation appears feasible.
2010-06-22 | GSE20532 | GEO
Project description:Unrestricted somatic stem cells (USSCs) from human cord blood show distinct differences to multipotent stromal cells isolated from human bone marrow and placenta both at the gene array and functional level. Fibroblast samples and raw data also included in E-TABM-724.
2014-11-26 | E-TABM-880 | biostudies-arrayexpress
Project description:Global gene expression analysis of (a) human embryonic stem cells, (b) adult fibroblasts with and without nucleofection of SOKM, (c) CD34+ cord blood cells at various time points during induction of pluripotency with SOKM, with or without co-culture with bone marrow stromal cells (BMSC), and (d) resulting stromal primed and non-stromal primed cord blood CD34+ myeloid iPSC
2016-09-13 | GSE44425 | GEO
Project description:Global gene expression analysis of (a) human embryonic stem cells, (b) adult fibroblasts with and without nucleofection of SOKM, and ( c ) CD34+ cord blood cells at various time points during induction of pluripotency with SOKM, with or without co-culture with bone marrow stromal cells (BMSC).
2012-08-23 | GSE35027 | GEO
Project description:This SuperSeries is composed of the following subset Series: GSE35027: Global gene expression analysis of human embryonic stem cells, adult fibroblasts , and CD34+ cord blood (CB) cells before, during, and afer their episomal induction of pluripotency GSE35028: Global gene expression analysis of pluripotent cell lines and corresponding starting donor source cells Refer to individual Series
2012-08-08 | E-GEOD-35029 | biostudies-arrayexpress
Project description:The contribution of microRNA-mediated posttranscriptional regulation on the final proteome in differentiating cells remains elusive. Here, we evaluated the impact of microRNAs (miRNAs) on the proteome of human umbilical cord blood-derived unrestricted somatic stem cells (USSC) during retinoic acid (RA) differentiation by a systemic approach using next generation sequencing analysing mRNA and miRNA expression and quantitative mass spectrometry-based proteome analyses. Interestingly, regulation of mRNAs and their dedicated proteins highly correlated during RA-incubation. Additionally, RA-induced USSC demonstrated a clear separation from native USSC thereby shifting from a proliferating to a metabolic phenotype. Bioinformatic integration of up- and downregulated miRNAs and proteins initially implied a strong impact of the miRNome on the XXL-USSC proteome. However, quantitative proteome analysis of the miRNA contribution on the final proteome after ectopic overexpression of downregulated miR-27a-5p and miR-221-5p or inhibition of upregulated miR-34a-5p, respectively, followed by RA-induction revealed only minor proportions of differentially abundant proteins. In addition, only small overlaps of these regulated proteins with inversely abundant proteins in non-transfected RA-treated USSC were observed. Hence, mRNA transcription rather than miRNA-mediated regulation is the driving force for protein regulation upon RA-incubation, strongly suggesting that miRNAs are fine-tuning regulators rather than active primary switches during RA-induction of USSC.
2020-01-31 | PXD016915 | Pride
Project description:Induction of pluripotency in somatic cells needs the full activation of the pluripotency gene regulatory network (PGRN). The core transcription factors of the PGRN establish a crosstalk with specific micro RNA (miRNA) families to sustain the pluripotent program and govern cell fate decisions. Very recently, circular RNA (circRNA) have been proposed as novel players in the regulation of this molecular circuitry. Herein, we successfully generated human induced pluripotent stem cells (hiPSC) by zero-footprint reprogramming of cord blood mesenchymal stem cells. The hiPSC were extensively characterized for stemness and tri-lineage differentiation potential compared to human embryonic stem cells and parental unreprogrammed cells to assess complete acquisition of the pluripotent identity. High-throughput array-based molecular analyses of messenger RNA (mRNA) (631 genes), miRNA (754 miRNA) and, for the first time, circRNA (13,617 circRNA) were performed to address the role of circRNA in the PGRN. As a result, a circRNA-guided map of miRNA and mRNA associated to naïve and primed pluripotent identity is provided.
2020-07-07 | GSE144629 | GEO
Project description:Induction of pluripotency in somatic cells needs the full activation of the pluripotency gene regulatory network (PGRN). The core transcription factors of the PGRN establish a crosstalk with specific micro RNA (miRNA) families to sustain the pluripotent program and govern cell fate decisions. Very recently, circular RNA (circRNA) have been proposed as novel players in the regulation of this molecular circuitry. Herein, we successfully generated human induced pluripotent stem cells (hiPSC) by zero-footprint reprogramming of cord blood mesenchymal stem cells. The hiPSC were extensively characterized for stemness and tri-lineage differentiation potential compared to human embryonic stem cells and parental unreprogrammed cells to assess complete acquisition of the pluripotent identity. High-throughput array-based molecular analyses of messenger RNA (mRNA) (631 genes), miRNA (754 miRNA) and, for the first time, circRNA (13,617 circRNA) were performed to address the role of circRNA in the PGRN. As a result, a circRNA-guided map of miRNA and mRNA associated to naïve and primed pluripotent identity is provided.
2020-07-07 | GSE144634 | GEO
Project description:Induction of pluripotency in somatic cells needs the full activation of the pluripotency gene regulatory network (PGRN). The core transcription factors of the PGRN establish a crosstalk with specific micro RNA (miRNA) families to sustain the pluripotent program and govern cell fate decisions. Very recently, circular RNA (circRNA) have been proposed as novel players in the regulation of this molecular circuitry. Herein, we successfully generated human induced pluripotent stem cells (hiPSC) by zero-footprint reprogramming of cord blood mesenchymal stem cells. The hiPSC were extensively characterized for stemness and tri-lineage differentiation potential compared to human embryonic stem cells and parental unreprogrammed cells to assess complete acquisition of the pluripotent identity. High-throughput array-based molecular analyses of messenger RNA (mRNA) (631 genes), miRNA (754 miRNA) and, for the first time, circRNA (13,617 circRNA) were performed to address the role of circRNA in the PGRN. As a result, a circRNA-guided map of miRNA and mRNA associated to naïve and primed pluripotent identity is provided.
2020-07-07 | GSE144631 | GEO