Project description:Cholangiocarcinoma (CCA) is a dreaded complication of primary sclerosing cholangitis (PSC), difficult to diagnose and associated with high mortality. Lack of animal models of CCA recapitulating the hepatic microenvironment of sclerosing cholangitis hinders development of novel treatments. We have developed and characterized a new mouse model of PSC-CCA through hydrodynamic tail vein injection of oncogenes pT3-EF1a-HA-myrAKT (AKT) and pT3-EF1a-YapS127A (YAP1), termed SB CCA.Mdr2-/-, which features reliable tumor induction in PSC-like background of biliary injury and fibrosis. To unravel the potential molecular profile of CCA developed in different liver context, we profiled the transcriptomes of tumor-bearing fibrotic (Mdr2-/-) (n = 6) and tumor-bearing wild-type liver tissues (n = 6) and compared to corresponding control nontumor-bearing fibrotic (Mdr2-/-) (n = 6) and healthy liver tissues (n = 6). RNA-seq analysis revealed profound transcriptional differences in CCA evolving in PSC-like context, compared to CCA in healthy liver.
2024-06-03 | GSE268905 | GEO
Project description:A Neonatal Infection Case Report
| PRJNA1456760 | ENA
Project description:Case Report: Clinical and Genetic features in a case of pediatric choroidal melanoma
Project description:Primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC), and inflammatory bowel diseases (IBDs), including Crohn’s disease (CD) and ulcerative colitis (UC), are heterogeneous chronic autoimmune diseases that may share underlying pathogenic mechanisms. Herein, we compared simultaneously analyzed blood transcriptomes from patients with PBC, PSC, and IBD.
2019-05-01 | GSE119600 | GEO
Project description:Case Report: Multimodal optical imaging and genetic features of AB variant GM2 gangliosidosis
Project description:Background: Primary sclerosing cholangitis (PSC) is an autoimmune, cholestatic liver disease characterized by inflammation and fibrosis surrounding interlobular bile ducts. The cellular composition and cell-cell-communications driving periductal fibrosis remain ill defined.
Project description:Two diagnostic liver biopsy specimens from pediatric patients with primary sclerosing cholangitis were profiled by single-cell spatial transcriptomics using the VizGen MERSCOPE platform with a custom 400-gene liver immunology panel. The material consisted of freshly cut, unstained sections from archived FFPE needle biopsies mounted on VizGen-provided slides for hybridization according to the manufacturer’s protocol. Both specimens contained focal periductal and bridging fibrosis.
2026-04-15 | GSE325861 | GEO
Project description:Dietary intervention on Primary Sclerosing Cholangitis
| PRJNA1108337 | ENA
Project description:Case report: Novel genetic variant associated with epilepsy