Project description:The equine amniotic membrane is a critical barrier to the amniotic compartment and may contribute to surfactant synthesis. To investigate its role in ascending placentitis, we used RNA sequencing, bioinformatics, and immunohistochemistry to analyze gene expression in amniotic membranes from experimentally induced placentitis cases (n = 6) and gestationally matched controls (n = 6). We identified 288 differentially expressed genes (DEGs), with upregulation of TLR4, PTGS2, PTGES, MMP9, CASP3, SOD2, BNIP3, and HMOX1 in placentitis. Immunohistochemistry confirmed protein expression of TLR4 and PTGS2. Functional annotation revealed enrichment in toll-like receptor signaling, suggesting immune activation may impair placental function. These findings highlight the amniotic membrane’s active role in immune response during placentitis.
Project description:Placentitis was induced in six mares at approximately 290d of gestation (placentitis group), and six mares with gestationally age-matched (290 d) pregnancies did not receive any treatment (control group). For induction of placentitis in the former group, Streptococcus equi subsp. zooepidemicus was introduced intracervically.
Project description:Placentitis was induced in five mares at approximately 290d of gestation (placentitis group), four mares with gestationally age-matched (290 d) pregnancies did not receive any treatment (control group), and the remaining three mares were maintained until approximately 330 d of gestation (prepartum group). For induction of placentitis in the former group, Streptococcus equi subsp. zooepidemicus was introduced intracervically.
Project description:Placentitis was induced in five mares at approximately 290d of gestation (placentitis group), four mares with gestationally age-matched (290 d) pregnancies did not receive any treatment (control group), and the remaining three mares were maintained until approximately 330 d of gestation (prepartum group). For induction of placentitis in the former group, Streptococcus equi subsp. zooepidemicus was introduced intracervically.
Project description:Placentitis was induced in five mares at approximately 290d of gestation (placentitis group), four mares with gestationally age-matched (290 d) pregnancies did not receive any treatment (control group), and the remaining three mares were maintained until approximately 330 d of gestation (prepartum group). For induction of placentitis in the former group, Streptococcus equi subsp. zooepidemicus was introduced intracervically.
Project description:Equine lameller tissues were collected to compare normal vs laminitis generated differences in transcriptom level. Keywords: Laminitis, Equine, Diseased foot