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Nicotiana benthamiana

ABSTRACT: Magnetic sorting of plant cells

PROVIDER: PRJNA1254296 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR33755900.fastq.gz	Fastqsanger.gz
	SRR33755901.fastq.gz	Fastqsanger.gz
	SRR33755902.fastq.gz	Fastqsanger.gz
	SRR33755903.fastq.gz	Fastqsanger.gz
	SRR33755904.fastq.gz	Fastqsanger.gz

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Identification of potential human PML interacting proteins in Nicotiana benthamiana

Project description:In spite of the crucial tasks fulfilled by the subnuclear structures known as PML nuclear bodies (PML-NBs) in mammalian cells, the presence of PML-NBs in plant cells could not be shown yet. Interestingly, the key organizer of PML-NBs, human promyelocytic leukemia protein (PML), is not targeted to structures resembling PML-NBs when expressed heterologously in plants, but localizes to interchromosomal areas associated with the nucleolus. In order to identify plant proteins interacting with human PML, a GFP-fusion protein was ectopically expressed in Nicotiana benthamiana using Agrobacterium tumefaciens mediated transformation. The bait protein as well as potential interactors were immunoprecipitated by use of magnetic GFP-Trap beads (Chromotek). Precipitated proteins were analyzed by Nano-LC-MS/MS.

2016-10-10 | PXD004254 | Pride

RNAseq of Colletotrichum orbiculare on Nicotiana benthamiana

Project description:Time course of C. orbiculare infection of the host plant, N. benthamiana, and comparison to in vitro grown tissue (conidia and in vitro grown hyphae) to identify genes that contribute to the infection process by transcript expression profile.

2021-10-26 | GSE178879 | GEO

Small RNA sequencing of NlCSP1-infiltrated Nicotiana benthamiana

Project description:In order to find the miRNAs, target genes and signaling pathways related to the dwarf phenotype induced by NlCSP1, 35S-NlCSP1 and 35S-GFP control were infiltrated into Nicotiana benthamiana. We analyzed the differentially expressed miRNAs in the infiltrated leaves (NlCSP1_Z/GFP_Z) and the upper uninfiltrated leaves (NlCSP1_O/GFP_O) of N. benthamiana. A total of 106 and 97 differentially expressed miRNAs were identified in NlCSP1_Z/GFP_Z and NlCSP1_O/GFP_O, respectively. KEGG annotation revealed a large number of differentially expressed miRNAs involved in plant hormone signal transduction, indicating that NlCSP1 changed the hormone signal pathway in N. benthamiana.

2021-09-01 | GSE169282 | GEO

Nicotiana benthamiana

Project description:Distinct genome-wide R-loop patterns and RNA dynamics during plant innate immune response

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Project description:Raw sequence reads of M1 shoots of regenerated Nicotiana benthamiana plant lines containing PDS gene editing

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The transcriptional landscape of Nicotiana benthamiana and Verticillium dahliae interaction

Project description:Verticillium dahliae is a soil-borne fungus with a broad host range, including the model plants Nicotiana benthamiana and Arabidopsis thaliana. The plant immunity can be activated by Vd-derived patterns while V. dahliae secreted effectors. We report here the RNAseq analyses of samples from N. benthamiana infected by V. dahliae strains 592 and 171 at different time points. The data showed dynamic expression patterns of genes not only from plant defense signaling but also plant developmental signaling.

2023-07-13 | GSE236729 | GEO

RNA sequencing of NlCSP11-infiltrated Nicotiana benthamiana

Project description:In order to find the differentially expressed RNAs and signaling pathways related to the dwarf phenotype induced by NlCSP11, 35S-NlCSP11, 35S-XopQ and 35S-GFP control were infiltrated into Nicotiana benthamiana. We analyzed the differentially expressed RNAs in the infiltrated leaves (NlCSP11_Z/XopQ_Z) and the upper uninfiltrated leaves (NlCSP11_U/XopQ_U) of N. benthamiana. KEGG annotation revealed a large number of differentially expressed RNAs involved in plant-pathogen interactions, indicating that NlCSP11 resulted in a more strong systemic resistance in N. benthamiana.

2024-05-30 | GSE267686 | GEO

Nicotiana Benthamiana

Project description:pssRNAit: a web server for designing highly effective and specific plant RNAi siRNAs with genome-wide off-target gene assessment

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Strigolactones negatively regulate Tobacco mosaic virus resistance in Nicotiana benthamiana

Project description:Strigolactones (SLs) are plant hormones that regulate diverse developmental processes and environmental responses in plants. It has been discovered that SLs play an important role in regulating plant immune resistance to pathogens, but there are currently no reports on their role in the interaction between Nicotiana benthamiana and Tobacco mosaic virus (TMV). In this study, the exogenous application of SLs weakened the resistance of N. benthamiana to TMV, promoting TMV infection, whereas the exogenous application of Tis108, an SL inhibitor, resulted in the opposite effect. Virus-induced gene silencing (VIGS) inhibition of two key SL synthesis enzyme genes, NtCCD7 and NtCCD8, enhanced the resistance of N. benthamiana to TMV. Additionally, we conducted a screening of N. benthamiana related to TMV infection. TMV-infected plants treated with SLs were compared to the control by using RNA-seq. KEGG enrichment analysis and weighted gene co-expression network analysis (WGCNA) of differentially expressed genes (DEGs) suggested that plant hormone signaling transduction may play a significant role in the SL-TMV-N. benthamiana interactions. This study reveals new functions of SLs in regulating plant immunity and provides a reference for controlling TMV diseases in production.

2024-08-16 | GSE272661 | GEO

Dual-transcriptomic analysis of Nicotiana benthamiana and two compatible Pseudomonas strains

Project description:We sought to compare and contrast plant host and bacterial transcriptional changes during compatible infections that cause disease (albeit within different symptoms). We investigated the infection by the two Pseudomonas syringae sensu lato strains P. syringae pv. syringae B728a (Psy) and P. amygdali pv. tabaci 11528 (Pta) of Nicotiana benthamiana at an early time point post inoculation to understand how a plant host responds to two related bacteria with different infection strategies. Plant and bacterial transcriptomes were analyzed prior to and five hours post inoculation.

2023-09-01 | GSE201377 | GEO

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