Project description:Two PacBio Hifi sequencing runs from the kidney of a single male NMR sample used to make the mHetGla4.1.primary genome assembly. Specifically, we assembled a second NMR genome from an unrelated male of a separate captive colony in Toronto, Canada, using PacBio HiFi (155.8 Gb, read N50 = 11.45 Kb) and ONT-LSK (299.6 Gb, read N50 = 10.1 Kb) reads (contig N50 = 75.7 Mb, Compleasm S = 98%). This accession stores the Pacbio Hifi data for this independent assembly.
Project description:One PacBio Hifi sequencing run from the kidney of a single male CDMR (Bathyergus suillus) sample used to make the mBatSui1.1.primary genome assembly as an evolutionary comparator to our telomere-to-telomere naked mole-rat genome assembly. Specifically, we assembled a CDMR from a wild-derived sample in South African cape and sequenced in Toronto, Canada, using PacBio HiFi (89 Gb, read N50 = 18 Kb) and ONT-ULK (55 Gb, read N50 = 43 Kb) reads (contig N50 = 33 Mb, Compleasm S = 99%, QV = 71.0). This accession stores the Pacbio Hifi data for this assembly.
Project description:This project focuses both on the separate and combined effects of large tidal volume ventilation and hyperoxia on gene expression in the lungs of anesthetized rats. Rats were either mechanically or spontaneously ventilated at either 21 or 100% oxygen and expression levels were evaluated on RG_U34 GeneChips. Keywords: other
Project description:In the present study we seek to identify changes in lung gene expression under mechanical ventilation in uninjured as well as acutely and chronically injured lungs. A standard volume-controlled lung-protective ventilatory protocol is compared to a concept of mechanical ventilation using variable tidal volumes.
Project description:Brain injury in extremely premature infants (gestational age <32 weeks) causes lifelong neurological sequelae, but the differentially expressed plasma proteins remains unclear. Nineteen extremely premature infants were enrolled, including 10 with brain injury and 9 without brain injury. Plasma proteomic sequencing were performed. Differential proteins were identified using t test analysis, followed by enrichment analyses. univariate logistic regression and LASSO were applied to identify informative clinical variables. Forty-four differential proteins were identified, which were mainly enriched in cytoskeleton-related functions. Four hub characteristic proteins were screened, including phosphoglycerate kinase 2 (PGK2), metallothionein, C-myc promoter-binding protein (MYCPP), and peroxisomal trans-2-enoyl-CoA reductase (PECR).