Project description:This project focuses both on the separate and combined effects of large tidal volume ventilation and hyperoxia on gene expression in the lungs of anesthetized rats. Rats were either mechanically or spontaneously ventilated at either 21 or 100% oxygen and expression levels were evaluated on RG_U34 GeneChips. Keywords: other
Project description:In the present study we seek to identify changes in lung gene expression under mechanical ventilation in uninjured as well as acutely and chronically injured lungs. A standard volume-controlled lung-protective ventilatory protocol is compared to a concept of mechanical ventilation using variable tidal volumes.
Project description:Mechanical ventilation (MV) with high positive end-expiratory pressure (PEEP) is linked to ventilation-induced diaphragm dysfunction (VIDD), but the role of integrin beta-1 (ITGB1) in PEEP-associated diaphragm fibrosis remains unclear.
Project description:Brain injury in extremely premature infants (gestational age <32 weeks) causes lifelong neurological sequelae, but the differentially expressed plasma proteins remains unclear. Nineteen extremely premature infants were enrolled, including 10 with brain injury and 9 without brain injury. Plasma proteomic sequencing were performed. Differential proteins were identified using t test analysis, followed by enrichment analyses. univariate logistic regression and LASSO were applied to identify informative clinical variables. Forty-four differential proteins were identified, which were mainly enriched in cytoskeleton-related functions. Four hub characteristic proteins were screened, including phosphoglycerate kinase 2 (PGK2), metallothionein, C-myc promoter-binding protein (MYCPP), and peroxisomal trans-2-enoyl-CoA reductase (PECR).