Project description:CyTOF data showed that 3-HAA significantly increased the percentage of F4/80hiCX3CR1loKi67loMHCIIhi macrophage and decreased the percentage of F4/80loCD64+PD-L1lo macrophages. scRNA-seq analyses demonstrated that 3-HAA administration was proved to regulate the function of M1 macrophages, M2 macrophages, and proliferating macrophages.
Project description:Precise regional patterning is fundamental to tissue organization, yet the spatial logic that governs it remains poorly defined for many tissues. In the vertebrate retina, molecular domains along the dorsoventral and nasotemporal axes provide positional cues for regional specializations such as the high-acuity area (HAA). We combined multiplexed in situ hybridization data with single-cell transcriptomic data to create quantitative two-dimensional maps of developing retinal cells. In the developing chicken retina, this approach resolved sharp expression boundaries of genes involved in patterning, and revealed novel candidates enriched in the anlagen of the HAA. Comparative analysis of chicken, mouse, and human data demonstrated conserved axis-based programs, but distinct fine-scale organization consistent with presence/absence of an HAA. Here, we show that spatial reconstruction from scRNA-seq data, anchored by experimental benchmarks, enables comparative 2D topographic mapping of gene expression across species and provides a generalizable strategy to investigate the spatial logic of molecular organization in developing tissues.
Project description:To clarify the mechanisms of cyclophosphamide (CY)-induced myocardial damage, we have analyzed the genome-wide expression profiles of C57BL/6J mice received CY, one of CY metabolites acrolein, and CY with N-acetyl cysteine (NAC). The microarray analysis revealed that the gene expression of L-type calcium channel (DHPR), ryanodine receptor 2 (RyR2), and Troponin C (TnC) were suppressed by CY administration. DHPR, RyR2 and TnC are responsible for control of intracellular calcium ion concentration and important for myocardial contraction. On the other side, the gene expression profiles after administration of acrolein differed from CY administration. Furthermore, the gene expression profiles after administration of NAC and CY, NAC did not inhibit the DHPR, RyR2 and TnC gene expression suppression.