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Vagal TRPV1+ sensory neurons regulate myeloid cell dynamics and protect against influenza virus infection

ABSTRACT: Vagal TRPV1+ sensory neurons regulate myeloid cell dynamics and protect against influenza virus infection

PROVIDER: PRJNA1257429 | ENA |

REPOSITORIES: ENA

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Vagal TRPV1+ sensory neurons regulate myeloid cell dynamics and protect against influenza virus infection

Project description:Influenza viruses are a major global cause of morbidity and mortality. While vagal TRPV1+ nociceptive sensory neurons are known to mediate defenses against harmful agents, their function in lung antiviral defenses remains unclear. Our study reveals that both systemic and vagal-specific ablation of TRPV1+ nociceptors reduce survival in mice infected with influenza A virus (IAV). Despite no difference in viral load, mice lacking TRPV1+ neurons exhibited increased viral spread, exacerbated lung pathology, and elevated levels of pro-inflammatory cytokines. Loss of TRPV1+ neurons altered the lung immune landscape, including an expansion of neutrophils and monocyte-derived macrophages. Transcriptional analysis revealed impaired interferon signaling in these myeloid cells and an imbalance in distinct neutrophil sub-populations in the absence of nociceptors. Furthermore, antibody-mediated depletion of myeloid cells during IAV infection significantly improved survival, underscoring a role of TRPV1+ nociceptors in preventing pathogenic myeloid cell states that contribute to IAV-induced mortality.

2025-08-01 | GSE296065 | GEO

The impact of influenza pulmonary infection and inflammation on vagal bronchopulmonary sensory neurons

Project description:Influenza A virus (IAV) is rapidly detected in the airways by the immune system, with resident parenchymal cells and leukocytes orchestrating viral sensing and the induction of antiviral inflammatory responses. The airways are innervated by heterogenous populations of vagal sensory neurons which also play an important role in pulmonary defense. How these neurons respond to IAV respiratory infection remains unclear. Here, we use a murine model to provide the first evidence that vagal sensory neurons undergo significant transcriptional changes following a respiratory IAV infection. RNA sequencing on vagal sensory ganglia showed that IAV infection induced the expression of many genes associated with an antiviral and pro-inflammatory response

2021-06-20 | GSE161878 | GEO

Expression of receptors for inflammatory mediators in murine vagal nodose versus jugular capsaicin-sensitive (Trpv1-positive) C-fiber neurons

Project description:Capsaicin-sensitive (Trpv1-positive) sensory C-fibers derived from vagal ganglia innervate the visceral organs, and respond to inflammatory mediators and noxious stimuli. These neurons play an important role in maintenance of visceral homeostasis, and contribute to the symptoms of visceral inflammatory diseases. Vagal sensory neurons are located in two ganglia, the jugular ganglia (derived from the neural crest), and the nodose ganglia (from the epibranchial placodes). The functional difference, especially in response to immune mediators, between jugular and nodose neurons is not fully understood. In this study, we microscopically isolated murine nodose and jugular capsaicin-sensitive / Trpv1-expressing C-fiber neurons and performed transcriptome profiling using ultra-low input RNA sequencing.

2017-10-01 | GSE102123 | GEO

The transcriptome of TRPV1-positive sensory neurons revealed by subgroup-elimination transcriptomics

2015-12-30 | E-MTAB-2789 | biostudies-arrayexpress

The transcriptome of TRPV1-positive sensory neurons revealed by subgroup-elimination transcriptomics

Project description:We developed an approach to rapidly eliminate the subgroup of sensory neurons expressing the heat-gated cation channel TRPV1 from dissociated rat sensory ganglia using agonist treatment followed by density centrifugation. To identify transcripts predomintly expressed in TRPV1-positive neurons, we compared the transcriptome of all cells within sensory ganglia versus all cells without TRPV1 expressing neurons using RNA-Seq. Four replicate experiments with RNA from DRG neurons of one rat per experiment were performed. Dissociated neurons were split up in three parts, treated with solvent DMSO (0.1%), casaicin (10 µM), or RTX (100 nM) for 30 min followed by gradient centrifugation. RNA was extracted from the remaining pellet containing either all cells or all cells without TRPV1-positive neurons.

2015-05-12 | E-GEOD-59727 | biostudies-arrayexpress

The transcriptome of TRPV1-positive sensory neurons revealed by subgroup-elimination transcriptomics

2015-05-12 | GSE59727 | GEO

Transcriptional profiling of individual airway projecting vagal sensory neurons

Project description:Mammalian airways and lungs are richly innervated by bronchopulmonary sensory neurons, the vast majority of which are derived from the vagal sensory ganglia. In the present study we set out to perform high coverage single cell RNA sequencing on a population of identified murine bronchopulmonary sensory neurons collected from the vagal sensory ganglia to better define the molecular expression profiles of these cell types. Given the importance of P2X2 in differentiating nodose from jugular sensory neurons, we further aimed to investigate the relationship between transcriptional expression of identified genes and P2X2 expression.

2019-07-16 | GSE134260 | GEO

Spatial transcriptomic analysis of liver-innervating vagal sensory neurons

Project description:We report the effect of ablating liver-innervating vagal sensory neurons in diet-induced obese male mice

2024-12-23 | GSE264570 | GEO

Single nucleus RNA sequencing analysis of vagal sensory neurons

Project description:We report that molecularly distinct populations of vagal sensory neurons would play a role in causing differences in metabolic homeostasis between the sexes.

2024-05-10 | GSE267231 | GEO

The impact of influenza pulmonary infection and inflammation on vagal bronchopulmonary sensory neurons

Project description:The impact of influenza pulmonary infection and inflammation on vagal bronchopulmonary sensory neurons

| PRJNA679770 | ENA