Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1259695 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR33457425_1.fastq.gz | Fastqsanger.gz | |||
| SRR33457425_2.fastq.gz | Fastqsanger.gz |
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Project description:Arthrobacter chlorophenolicus A6 is a 4-chlorophenol degrading soil bacterium with high phyllosphere colonization capacity. Till now the genetic basis for the phyllosphere competency of Arthrobacter or other pollutant-degrading bacteria is uncertain. We investigated global gene expression profile of A. chlorophenolicus grown in the phyllosphere of common bean (Phaseolus vulgaris) compared to growth on agar surfaces. We designed transcriptome arrays and investigated which genes had different transcript levels in the phyllosphere of common bean (Phaseolus vulgaris) as compared to agar surfaces. Since water availability is considered an important factor in phyllosphere survival and activity, we included both high and low relative humidity treatments for the phyllosphere-grown cells. In addition, we determined the expression profile under pollutant exposure by the inclusion of two agar surface treatments, i.e. with and without 4-chlorophenol.
2013-06-21 | E-GEOD-48198 | biostudies-arrayexpress
Project description:Arthrobacter chlorophenolicus A6 is a 4-chlorophenol degrading soil bacterium with high phyllosphere colonization capacity. Till now the genetic basis for the phyllosphere competency of Arthrobacter or other pollutant-degrading bacteria is uncertain. We investigated global gene expression profile of A. chlorophenolicus grown in the phyllosphere of common bean (Phaseolus vulgaris) compared to growth on agar surfaces.
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Project description:The NBRC Genome Sequencing Project for Pollutant-degrading bacteria
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Project description:This study examines the genomic effects of dieldrin in the hypothalamus of largemouth bass. Dieldrin is an insectide and organic pollutant.
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Project description:<p>Plant-microbe interactions in the phyllosphere have been demonstrated to facilitate plant growth and enhance resistance to diverse stresses; nevertheless, their role in the bioaccumulation of contaminants by plants is rarely considered. Here, by comparing differences in the phyllosphere morphology, bacterial community assembly and metabolites between two choysum (Brassica parachinensis) varieties differing in di-n-butyl phthalate (DBP) accumulation, we revealed the pivotal roles of key phyllosphere exudate (fumarate) and recruited specific microbes in determining the variety-specific DBP accumulation in choysum. The low-DBP accumulation variety (LAV) enhanced the recruitment of phyllosphere microbes capable of degrading DBP by increasing fumarate secretion, relative to the high-DBP accumulation variety (HAV), thereby facilitating the colonization of DBP-degrading bacteria via the induction of biofilm formation and ultimately resulting in lower DBP accumulation in leaves. These findings offer novel insights into the LAV formation from the phyllosphere microbial perspective and highlight the role of phyllosphere microbes in mediating pollutant accumulation within crops, which is instrumental in minimizing pollutant accumulation through regulating the phyllosphere microbial community.</p>
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Project description:Dichloromethane (DCM, methylene chloride) is a toxic halogenated volatile organic compound massively used for industrial applications, and consequently often detected in the environment as a major pollutant. DCM biotransformation offers a sustainable decontamination strategy of polluted sites. Among methylotrophic bacteria able to use DCM as sole source of carbon and energy for growth, Methylorubrum extorquens DM4 (formerly named Methyobacterium extorquens) is a longstanding reference Alphaproteobacteria strain. Here, its primary transcriptome was obtained using a differential RNA-seq (dRNA-seq) approach to provide the first transcription start site (TSS) genome-wide landscape of a methylotroph using DCM.
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