Telenomus remus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1260750 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Telenomus remus isolate:Trem-ZJU Genome sequencing and assembly
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Project description:<p>Telenomus remus, an egg parasitoid capable of penetrating multi-layered egg masses, has emerged as a promising candidate for the biological control of Spodoptera frugiperda. Nevertheless, our prior investigations have uncovered that its limited cold tolerance represents a critical bottleneck in mass rearing. To overcome this challenge, the overarching objectives were to identify candidate genes and metabolites associated with cold tolerance, investigate the dynamic changes of cryoprotectants under different stress conditions, and elucidate the cold tolerance mechanisms of T. remus. The results revealed that the survival rates of T. remus declined progressively as temperature decreased. Notably, a significant accumulation of trehalose was observed as the stress temperature decreased. Integrated multi-omics analysis indicated that the starch and sucrose metabolism pathway played a key role in mediating cold tolerance in T. remus. Within this metabolic pathway, the expression levels of GAA (α-glucosidase) and GYS (glycogen synthase) exhibited a clear temperature-dependent upregulation trend. Collectively, these findings suggest that T. remus adopted a cold-tolerance strategy centered around trehalose accumulation. These research advances our understanding of the molecular and biochemical foundations of cold adaptation in T. remus, while also highlighting trehalose-mediated osmotic regulation as a prioritized research direction for future ectotherm thermotolerance studies.</p>
2025-06-09 | MTBLS12473 | MetaboLights
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2024-02-08 | PXD049451 |
Project description:Purpose: The goal of this study is to compare endothelial small RNA transcriptome to identify the target of OASL under basal or stimulated conditions by utilizing miRNA-seq. Methods: Endothelial miRNA profilies of siCTL or siOASL transfected HUVECs were generated by illumina sequencing method, in duplicate. After sequencing, the raw sequence reads are filtered based on quality. The adapter sequences are also trimmed off the raw sequence reads. rRNA removed reads are sequentially aligned to reference genome (GRCh38) and miRNA prediction is performed by miRDeep2. Results: We identified known miRNA in species (miRDeep2) in the HUVECs transfected with siCTL or siOASL. The expression profile of mature miRNA is used to analyze differentially expressed miRNA(DE miRNA). Conclusions: Our study represents the first analysis of endothelial miRNA profiles affected by OASL knockdown with biologic replicates.
2022-09-06 | GSE186354 | GEO
Project description:A cDNA library was constructed by Novogene (CA, USA) using a Small RNA Sample Pre Kit, and Illumina sequencing was conducted according to company workflow, using 20 million reads. Raw data were filtered for quality as determined by reads with a quality score > 5, reads containing N < 10%, no 5' primer contaminants, and reads with a 3' primer and insert tag. The 3' primer sequence was trimmed and reads with a poly A/T/G/C were removed
2021-07-21 | GSE180131 | GEO