Antibacterial Effect of Epidemic Prevention Sachets Essential Oil against Staphylococcus Aureus
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ABSTRACT:
PROVIDER: PRJNA1261978 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR33536678_1.fastq.gz | Fastqsanger.gz | |||
| SRR33536678_2.fastq.gz | Fastqsanger.gz | |||
| SRR33536679_1.fastq.gz | Fastqsanger.gz | |||
| SRR33536679_2.fastq.gz | Fastqsanger.gz | |||
| SRR33536680_1.fastq.gz | Fastqsanger.gz |
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Project description:Staphylococcus aureus (S. aureus) is one of the most dominant human pathogen, responsible for a variety of chronic and severe infections. As a classic traditional Chinese medicine (TCM) formula, Epidemic Prevention Sachets have been used clinically for centuries to treat infectious diseases. The antimicrobial activity of the essential oil (EO) from Epidemic Prevention Sachets against the S. aureus has not been investigated. In this study, the EO was tested for its antimicrobial activity against S. aureus. We identified that the EOs has high activity against S. aureus. It showed higher activity than the known antibacterial drug combination of Penicillin streptomycin. The same results were demonstrated by transmission electron microscopy. The impact of the EO on transcription of genes from S. aureus was analyzed. SAOUHSC_01002, SAOUHSC_02444, SAOUHSC_00282 and SAOUHSC_00325, which are involved in cation transport (GO:0006812), were significantly affected by the essential oil as defined in the Gene Ontology Biological Process (GO BP), whereas SAOUHSC_00114 and SAOUHSC_03000, which are involved in the protein tyrosine kinase modulator pathway (K19420), were significantly enriched in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. Analysis of clusters of orthologous groups (COG) found that there are differences in multiple metabolic pathways, mainly including secondary biosynthesis metabolism, biofilm related genes and energy metabolism. Our study indicates that the essential oil from Epidemic Prevention Sachets is an effective herbal formula against S. aureus.
2025-05-17 | GSE296946 | GEO
Project description:Transcriptome analysis plays a central role in elucidating the complexity of gene expression regulation in Escherichia coli. By analyzing the transcriptomics of E. coli treated with water, acetone, and Cinnamomum camphora essential oil, the inhibitory mechanism of the essential oil on the human intestinal microbe was studied. The results showed that the inhibitory effect of the essential oil on E. coli increased with an increase in concentration; 1/4 minimum inhibitory concentration was the reaction equilibrium point. RNAseq transcriptomic comparison indicated that the essential oil inhibited the growth of E. coli by inhibiting the metabolism, chemotaxis, and some resistance reactions of them, while E. coli maintained its life activities by enhancing its resistance reactions. These results are of great importance to the study of the medical use of C. camphor essential oil and gene regulation in E. coli under stress conditions.
2020-10-29 | GSE160284 | GEO
Project description:RNAseq analysis of antibacterial mechanism of Cinnamomum camphora essential oil against Escherichia coli
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Project description:Proteome changes of Aspergillus flavus before and after adding essential oil treatment, discussing the antibacterial and enzyme activity inhibition mechanisms of essential oil
2023-11-01 | PXD046563 |
Project description:The present study aimed to investigate the antibacterial effect and mechanism of a carvacrol-rich Oregano essential oil (OEO) on the inactivation of Staphylococcus aureus. A label-free proteomic analysis was applied to analyze the regulatory networks of S. aureus in response to 1/2 MIC OEO-treatment stress. These findings deepened our understanding regarding S. aureus survival and metabolism responses to the OEO treatment and suggested that the carvacrol-rich OEO could be used in food production environments to effectively control S. aureus.
2021-11-02 | PXD028357 | Pride
Project description:The purpose of this study was to explore the mechanism of aerobic decay of whole-plant corn silage and the effect of Neolamarckia cadamba essential oil on aerobic stability of whole-plant corn silage. Firstly, the dynamic changes of temperature, microbial community and metabolite content after aerobic exposure of whole-plant corn silage were determined, and the main microbial species and mechanism leading to aerobic spoilage of whole-plant corn silage were analyzed. The N. cadamba essential oil was extracted from fresh N. cadamba leaves by steam distillation, and the minimal inhibitory concentration, antibacterial stability and bacteriostatic mechanism of N. cadamba essential oil against undesirable microorganisms in whole-plant corn silage were determined. According to the minimum inhibitory concentration of N. cadamba essential oil on undesirable microorganisms in silage, N. cadamba essential oil was added to whole-plant corn silage to explore the effect of N. cadamba essential oil on the aerobic stability of whole-plant corn silage.
2023-09-13 | GSE241081 | GEO
Project description:Type I interferons were discovered as the primary antiviral cytokines and are now known to serve critical functions in host defense against bacterial pathogens. Accordingly, established mediators of interferon antiviral activity may mediate previously unrecognized antibacterial functions. RNase-L is the terminal component of an RNA decay pathway that is an important mediator of interferon-induced antiviral activity. Here we identify a novel role for RNase-L in the host antibacterial response. RNase-L-/- mice exhibited a dramatic increase in mortality following challenge with Bacillus anthracis and Escherichia coli; this increased susceptibility was due to a compromised immune response resulting in increased bacterial load. Investigation of the mechanisms of RNase-L antibacterial activity indicated that RNase-L is required for the optimal induction of proinflammatory cytokines that play essential roles in host defense from bacterial pathogens. RNase-L also regulated the expression of the endolysosomal protease, cathepsin-E, and endosome-associated activities, that function to eliminate internalized bacteria and may contribute to RNase-L antimicrobial action. Our results reveal a unique role for RNase-L in the antibacterial response that is mediated through multiple mechanisms. As a regulator of fundamental components of the innate immune response, RNase-L represents a viable therapeutic target to augment host defense against diverse microbial pathogens. two strains: wildtype and knockout, three time points: untreated, 2hours, and 8hours. three replication for each group. Totally 18 samples.
2008-12-19 | E-GEOD-13530 | biostudies-arrayexpress
Project description:This transcriptional analysis is a follow up to a population genomic investigation of 3615 Streptococcus pyogenes serotype M1 strains whch are responsible for an epidemic of human invasive infections (www.pnas.org/cgi/doi/10.1073/pnas.1403138111), The goal was to assess gene expression differences between predecessor pre-epidemic M1 strains and their descendent epidemic M1 strains to gain insights into the underlying genetic basis for the shift in the frequency and severity of human infections caused by these pathogenic bacteria The transcriptomes of 7 GAS M1 strains, 4 pre-epidemic and 3 epidemic, were compared at two phases of growth, mid-exponential and early-stationary, using 3 biologial replicates, to identify genes differentially expressed between the pre-epidemic and epidemic isolates with the goal of to gaining insight into the underlying genetic basis for the evolutionary emergence, increased frequency and severity of the epidemic strains relative to the pre-epidemic strains
2015-08-10 | E-GEOD-70496 | biostudies-arrayexpress
Project description:The purpose of the present study was to establish at the molecular level, a genetic profile of the biological activity of essential sandalwood oil from Santalum album, produced under standardized conditions. The model system under investigation is based on living human skin explants. This protocol permits to compare ex vivo the effects of SO at the histological level as well as the changes of gene expression as a function of SO concentration and duration of treatment. In the present report, Sandalwood oil (S. album) was applied to living human skin explants to evaluate the sustained activity of the oil through gene expression profiling. Transcriptomic analysis showed a number of metabolic pathways and biological activities triggered by treatments previously described for aloe, curcumin and luteolin. No inflammatory responses accompanied the beneficial activities. The most intriguing impact was the natural effect on the retinoic acid (vitamin A) metabolism. Furthermore, we found that mechanisms normally acting in more specialized cell types such as immune cells, adipocytes, nerve cells, or hair follicles were activated in human skin explants in response to sandalwood oil. Thus, the oil may have the potential of further beneficial activities such as its antibacterial activity among other protective mechanisms. These results corroborate at a molecular level the rationale for clinical studies with sandalwood oil and support recent studies of patients with eczema (atopic dermatitis) or acne, which is often accompanied by bacterial growth on skin of Propionibacterium acnes.
2017-11-10 | GSE106734 | GEO
Project description:Objectives Pyrrolobenzodiazepine (PBD) dimers, tethered through inert propyldioxy or pentyldioxy linkers, possess potent bactericidal activity against a range of Gram-positive bacteria by virtue of their capacity to cross-link duplex DNA in sequence-selective fashion. Here we attempt to improve the antibacterial activity and cytotoxicity profile of PBD-containing conjugates by extension of dimer linkers and replacement of one PBD unit with phenyl-substituted or benzo-fused heterocycles that facilitate non-covalent interactions with duplex DNA. Methods DNase I footprinting was used to identify high-affinity DNA binding sites. A staphylococcal gene microarray was used to assess epidemic methicillin-resistant Staphylococcus aureus 16 phenotypes induced by PBD conjugates. Molecular dynamics simulations were employed to investigate the accommodation of compounds within the DNA helix. Results Increasing the length of the linker in PBD dimers led to a progressive reduction in antibacterial activity, but not in their cytotoxic capacity. Complex patterns of DNA binding were noted for extended PBD dimers. Modelling of DNA strand cross-linking by PBD dimers indicated distortion of the helix. A majority (26 of 43) of PBD-biaryl conjugates possessed potent antibacterial activity with little or no helical distortion and a more favourable cytotoxicity profile. Bactericidal activity of PBD-biaryl conjugates was determined by inability to excise covalently bound drug molecules from bacterial duplex DNA. Conclusions PBD-biaryl conjugates have a superior antibacterial profile compared with PBD dimers such as ELB-21. We have identified six PBD-biaryl conjugates as potential drug development candidates. [Data is also available from http://bugs.sgul.ac.uk/E-BUGS-118]
2012-04-17 | E-BUGS-118 | biostudies-arrayexpress