Project description:In this study, the complete mitogenome sequence of Pentacheles validus has been decoded for the first time. The overall base composition is 33.4% for A, 25.0% for C, 10.8% for G, and 30.9% for T and has low GC content of 35.8%. The assembled mitogenome, 16,079 bp in length, has unique 13 protein-coding genes (PCGs), 22 transfer RNAs and 2 ribosomal RNAs. The mitogenome shares 80% identity to Polycheles typhlops. The complete mitogenome of P. validus provides essential DNA molecular data for further phylogenetic and evolutionary analysis for Polychelidae and deep-sea faunas.
Project description:Leptodactylus validus is reported for the first time for Colombia, corresponding to the tenth species of the L. melanonotus species group occurring in the country. In collections, all L. validus specimens were identified as L. colombiensis. Morphological, coloration, and ecological characters are provided to differentiate the two species in Colombia. Furthermore, the distribution of L. validus is expanded based on the examination of specimens in both collections and literature records. In addition, the advertisement call of L. validus from Colombia is compared with those reported for other continental and insular populations; the calls are slightly more similar to those of insular populations.
Project description:Mutualistic interactions between organisms often mediate the innovation of traits essential to maintain the relationship. Yet our understanding of these interactions has been stymied due to various hurdles in studying the genetics of non-model animals. To understand the genetic mechanisms by which such traits develop, we examined the function of genes breathless (btl), trachealess (trh), and doublesex in the development of a novel fungus-carrying organ (mycangium) that facilitates an obligate relationship between fungus-farming ambrosia beetles and specific fungal partners. Gene knockdown by RNA interference and subsequent micro-computed tomography visualization suggest btl and trh are required for initiation of mycangia and that tubulogenesis may have been co-opted for early mycangial development.
| S-EPMC10462615 | biostudies-literature
Project description:The mitochondrial genomes of Enoplolambrus validus and Jonas distinctus
Project description:Bioprospecting of underexplored plants opens up new avenues for developing value added products, ready-to-use herbal formulations and nutraceuticals. This study investigates the volatile metabolome and mineral composition of Haematocarpus validus (Khoon phal), an ethnic fruit crop of tropical Asia. H. validus is used for curing hepatic diseases, anemic and inflammatory conditions in ethnic medicine. Analysis of EI mass spectra on GC-MS platform detected 54 and 48 compounds from fruit and leaf extract respectively. Quinic acid (a hepato-protectant) predominates among volatiles in fruit (29.13%) and unsaturated fatty acids in leaf (61%). Through HP-TLC spectrodensitometric study, quinic acid was quantified as 292.95 mg/100 g dry weight. Five macroelements and 17 microelements were analyzed using ICP-AES and ICP-MS respectively. H. validus fruit has very high K/Na ratio (280.65) and both fruit and leaf are rich in Fe content (57.29 and 38.44 mg/100 g dry weight respectively). High unsaturated fatty acid/ saturated fatty acid ratio and abundance of linoleic acid (55.54% of total fatty acids) in leaf along with relatively high content of conjugated linoleic acid suggest its potential usefulness in pharmaceutical and nutraceutical industry. This is the first report on volatile metabolome of H. validus fruit and leaf.Supplementary informationThe online version contains supplementary material available at 10.1007/s13197-021-04997-7.