Project description:In this study, the complete mitogenome sequence of Pentacheles validus has been decoded for the first time. The overall base composition is 33.4% for A, 25.0% for C, 10.8% for G, and 30.9% for T and has low GC content of 35.8%. The assembled mitogenome, 16,079 bp in length, has unique 13 protein-coding genes (PCGs), 22 transfer RNAs and 2 ribosomal RNAs. The mitogenome shares 80% identity to Polycheles typhlops. The complete mitogenome of P. validus provides essential DNA molecular data for further phylogenetic and evolutionary analysis for Polychelidae and deep-sea faunas.
Project description:Leptodactylus validus is reported for the first time for Colombia, corresponding to the tenth species of the L. melanonotus species group occurring in the country. In collections, all L. validus specimens were identified as L. colombiensis. Morphological, coloration, and ecological characters are provided to differentiate the two species in Colombia. Furthermore, the distribution of L. validus is expanded based on the examination of specimens in both collections and literature records. In addition, the advertisement call of L. validus from Colombia is compared with those reported for other continental and insular populations; the calls are slightly more similar to those of insular populations.
Project description:Co-expression networks and gene regulatory networks (GRNs) are emerging as important tools for predicting the functional roles of individual genes at a system-wide scale. To enable network reconstructions we built a large-scale gene expression atlas comprised of 62,547 mRNAs, 17,862 non-modified proteins, and 6,227 phosphoproteins harboring 31,595 phosphorylation sites quantified across maize development. There was little edge conservation in co-expression and GRNs reconstructed using transcriptome versus proteome data yet networks from either data type were enriched in ontological categories and effective in predicting known regulatory relationships. This integrated gene expression atlas provides a valuable community resource. The networks should facilitate plant biology research and they provide a conceptual framework for future systems biology studies highlighting the importance of studying gene regulation at several levels.