Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1268041 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR33708563_1.fastq.gz | Fastqsanger.gz | |||
| SRR33708563_2.fastq.gz | Fastqsanger.gz | |||
| SRR33708564_1.fastq.gz | Fastqsanger.gz | |||
| SRR33708564_2.fastq.gz | Fastqsanger.gz | |||
| SRR33708565_1.fastq.gz | Fastqsanger.gz |
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Project description:Early-weaning-induced stress causes diarrhea, thereby reduces growth performance of piglets. Gut bacterial dysbiosis emerges as a leading cause of post-weaning diarrhea. The present study was aimed to investigate the effect of capsulized fecal microbiota transportation (FMT) on gut bacterial community, immune response and gut barrier function of weaned piglets. Thirty-two were randomly divided into two groups fed with basal diet for 21 days. Recipient group was inoculated orally with capsulized fecal microbiota of health Tibetan pig daily morning during whole period of trial, while control group was given orally empty capsule. The results showed that the F/G ratio, diarrhea ratio, diarrhea index, and histological damage score of recipient piglets were significantly decreased. FMT treatment also significantly increased the colon length of piglets. Furthermore, the relative abundances of Firmicutes, Euryarchaeota, Tenericutes, Lactobacillus, Methanobrevibacter and Sarcina in colon of recipient piglets were increased, and the relative abundances of Campylobacter, Proteobacteria, and Melainabacteria were significantly decreased compared with control group.
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Project description:In this study, we examined Caco-2 cell gene expression after infection with E. coli (Ec), Lactobacillus plantarum (Lp) and the combination of the two (mix) Keywords: Lactobacillus plantarum and E. coli influences on Caco2 cells gene expression
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Project description:The brown seaweed Laminaria digitata is a novel feedstuff for weaned piglets. It can help prevent dysbiosis in addition to improve overall health and performance. However, it has a recalcitrant cell wall that is not easily digested by the piglet digestive system. Alginate lyase has promising effects for the supplementation of in vivo diets in order to address this issue. The objective of this study is to evaluate the effect of 10% dietary Laminaria digitata inclusion and alginate lyase supplementation on the hepatic proteome and metabolome of weaned piglets in a physiological study. The diets caused incipient differences on the metabolome of piglets, with the proteome having the most significant changes. Feeding seaweed provided a source of n-3 PUFA that accumulated in the liver, signalling for increased fatty acid oxidation (FABP, ACADSB, ALDH1B1). This contributed at least in part to reduce oxidative stability of the tissue, demanding the higher abundance of GST to maintain it. Reactive oxygen species possibly damaged proteins, which caused hepatocytes to increase proteasome activity (LAPTM4B, PSMD4), recycling their amino acids. Providing alginate lyase to the diet increased the number of differentially abundant proteins, including GBE1 and LDHC that contributed to the maintenance of circulating glucose through mobilization of glycogen stores and branched chain amino acids. Enzymatic supplementation enhanced the baseline effects of feeding seaweed alone.
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Project description:Seaweeds, including the green Ulva lactuca, can potentially reduce competition between feed, food, and fuel. They can also contribute to the improved development of weaned piglets. However, their indigestible polysaccharides of the cell wall pose a challenge. This can be addressed through carbohydrase supplementation, such as the recombinant ulvan lyase. The objective of our study was to assess the muscle metabolism of weaned piglets fed with 7% U. lactuca and 0.01% ulvan lyase supplementation, using an integrated transcriptomics and proteomics approach. Feeding piglets with seaweed and enzyme supplementation resulted in reduced macronutrient availability, leading to protein degradation through the proteasome (PSMD2), with resulting amino acids being utilized as an energy source (GOT2, IDH3B). Moreover, mineral element accumulation (iodine and bromine) contributed to increased oxidative stress, evident from elevated levels of antioxidant proteins like catalase, as a response to maintain tissue homeostasis. The upregulation of the gene AQP7, associated with the osmotic stress response, further supports these findings. Consequently, an increase in chaperone activity, including HSP90, was required to repair damaged proteins. Our results suggest that enzymatic supplementation may exacerbate the effects observed from feeding U. lactuca alone, potentially due to side effects arising from cell wall degradation during digestion.
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Project description:To investigate the effect of short distance transport on jejunal tissueof weaned piglets, We then performed gene expression profiling analysis using data obtained from RNA-seq in jejunal tissues of weaned piglets after transport and without transport
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Project description:Microbiome analysis has relied largely on metagenomics to characterize microbial populations and predict their functions. Here, we used a TMT LC-MSMS metaproteomic analysis of the fecal microbiome in piglets before and after weaning to compare protein abundances as they pertain to microbial populations specific to either a milk- or plant-based diet. Fecal samples were collected from six piglets on the day of weaning and four weeks after transitioning to a standard nursery diet. Using the 12,554 protein groups identified in samples, we confirmed the shift in protein composition that takes place in response to the microbial succession following weaning and demonstrated the redundancy in metabolic processes between taxa. We identified taxa with roles as primary degraders based on corresponding proteins synthesized, thereby providing evidence for cross-feeding. Proteins associated with the breakdown of milk-specific carbohydrates were common among pre-weaned pigs, whereas the proteome of post-weaned piglets contained a greater abundance of proteins involved in the breaking down plant-specific carbohydrates. Furthermore, output revealed that production of propionate takes place via the propionaldehyde pathway in pre-weaned piglets, but changes to production via the succinate pathway in post-weaned piglets. Finally, a disproportionate quantity of carbohydrate-active enzymes (CAZymes) (~8%) were produced by fungi, which typically only represent ~0.1% of the microbiome taxa. Information gathered through this characterization of the metaproteome before and after weaning revealed important differences regarding the role of members in the microbial community, thereby providing information for the optimization of diets and products for both piglet and microbiome health.
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