Project description:Gut microbiota were assessed in 540 colonoscopy-screened adults by 16S rRNA gene sequencing of stool samples. Investigators compared gut microbiota diversity, overall composition, and normalized taxon abundance among these groups.
Project description:The impact of mono-chronic S. stercoralis infection on the gut microbiome and microbial activities in infected participants was explored. The 16S rRNA gene sequencing of a longitudinal study with 2 sets of human fecal was investigated. Set A, 42 samples were matched, and divided equally into positive (Pos) and negative (Neg) for S. stercoralis diagnoses. Set B, 20 samples of the same participant in before (Ss+PreT) and after (Ss+PostT) treatment was subjected for 16S rRNA sequences and LC-MS/MS to explore the effect of anti-helminthic treatment on microbiome proteomes.
Project description:In this study, we performed a comparative analysis of gut microbiota composition and gut microbiome-derived bacterial extracellular vesicles (bEVs) isolated from patients with solid tumours and healthy controls. After isolating bEVs from the faeces of solid tumour patients and healthy controls, we performed spectrometry analysis of their proteomes and next-generation sequencing (NGS) of the 16S gene. We also investigated the gut microbiomes of faeces from patientsand controls using 16S rRNA sequencing. Machine learning was used to classify the samples into patients and controls based on their bEVs and faecal microbiomes.
Project description:To explore the effects of gut microbiota of young (8 weeks) or old mice (18～20 months) on stroke, feces of young (Y1-Y9) and old mice (O6-O16) were collected and analyzed by 16s rRNA sequencing. Then stroke model was established on young mouse receive feces from old mouse (DOT1-15) and young mouse receive feces from young mouse (DYT1-15). 16s rRNA sequencing were also performed for those young mice received feces from young and old mice.
Project description:This study aimed to elucidate the molecular gut-brain pathways underlying the anxiolytic effects of heat-inactivated L. brevis SBC8803 using zebrafish as a translational model. Adult fish received oral SBC8803 and were assessed in the novel tank test. To interrogate mechanisms, we combined brain RNA sequencing with 16S rRNA gene profiling of the gut microbiota and performed integrative multi‑omics analyses to identify host gene expression changes and microbial functional alterations associated with anxiolysis.
Project description:This study aimed to elucidate the molecular gut-brain pathways underlying the anxiolytic effects of heat-inactivated L. brevis SBC8803 using zebrafish as a translational model. Adult fish received oral SBC8803 and were assessed in the novel tank test. To interrogate mechanisms, we combined brain RNA sequencing with 16S rRNA gene profiling of the gut microbiota and performed integrative multi‑omics analyses to identify host gene expression changes and microbial functional alterations associated with anxiolysis.
Project description:What are the effects of Microcystis aeruginosa and cyanotoxin on the grass carp intestine? 16S rRNA sequencing is needed to analyze the gut microbiota.
Project description:Hepatic steatosis is the initial manifestation of abnormal liver functions and often leads to liver diseases such as non-alcoholic fatty liver disease in humans and fatty liver syndrome in animals. In this study, we conducted a comprehensive analysis of a large chicken population consisting of 705 adult hens by combining host genome resequencing, liver transcriptome, proteome, and metabolome analysis, as well as microbial 16S rRNA gene sequencing of each gut segment.
2024-04-09 | PXD042451 | Pride
Project description:ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples
