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ABSTRACT: RNA-seq for Cynoglossus semilaevis after Vibrio harveyi infection

PROVIDER: PRJNA1270417 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR33779222_1.fastq.gz	Fastqsanger.gz
	SRR33779222_2.fastq.gz	Fastqsanger.gz
	SRR33779223_1.fastq.gz	Fastqsanger.gz
	SRR33779223_2.fastq.gz	Fastqsanger.gz
	SRR33779224_1.fastq.gz	Fastqsanger.gz

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Project description:Vibrio harveyi is a major bacterial pathogen that can cause fatal vibriosis in Chinese tongue sole (Cynoglossus semilaevis). To comprehend the molecular mechanisms of C. semilaevis host response against V. harveyi infection, we performed transcriptome (RNA-seq) analysis of C. semilaevis from resistant family and susceptible family.

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Project description:RNA-Seq of Cynoglossus semilaevis

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Project description:Vibrio spp. are major pathogens responsible for mortality and disease in various marine aquaculture organisms. Effective disease control and genetic breeding strategies rely heavily on understanding host vibriosis resistance mechanisms. The Chinese tongue sole (Cynoglossus semilaevis) is economically vital but suffers from substantial mortalities due to vibriosis. Through continuous selective breeding, we have successfully obtained vibriosis-resistant families of this species. In this study, we conducted RNA-seq analysis on three organs, including liver, spleen and intestine from selected resistant and susceptible tongue soles. Additionally, we integrated these data with our previously published RNA-seq datasets of skin and gill, enabling the construction of organ-specific transcriptional profiles and a comprehensive gene co-expression network elucidating the differences in vibriosis resistance. Furthermore, we identified 12 modules with organ-specific functional implications. Overall, our findings provide a valuable resource for investigating the molecular basis of vibriosis resistance in fish, offering insights into target genes and pathways essential for molecular selection and genetic manipulation to enhance vibriosis resistance in fish breeding programs.

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