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Transcriptional regulon controlled by tyrosine phosphatases in Porphyromonas gingivalis

ABSTRACT: Transcriptional regulon controlled by tyrosine phosphatases in Porphyromonas gingivalis

PROVIDER: PRJNA1272481 | ENA |

REPOSITORIES: ENA

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Transcriptional regulon controlled by tyrosine phosphatases in Porphyromonas gingivalis

Project description:Tyrosine phosphorylation/dephosphorylation posttranslational modification (PTM) of proteins in bacteria can control their function and location. While PTM of transcriptional regulators and DNA-binding proteins impacts gene transcription, little is known regarding the transcriptional landscape regulating tyrosine phospho-relay circuitry. In this study, we performed RNA-Seq of Porphyromonas gingivalis strains with mutations in ltp1 and php1 genes encoding the two major tyrosine phosphatases, a low molecular weight family enzyme and a histidinol phosphate phosphoesterase family enzyme, respectively. Additionally, strains were tested in both in vitro and in vivo (mouse abscess) conditions. Both the Δltp1 and the Δphp1 mutants exhibit little transcriptional difference to the parental strain cultured in vitro, which would tend to exclude a significant role of the phosphatases in P. gingivalis gene regulation under nutrient-rich, low-stress conditions. While the Δphp1 mutant also clustered closely to the parental strain in the abscess condition, there were a number of differentially regulated genes (DEGs) associated with transporter systems. In the mouse abscess condition, DEGs in Δltp1 included one of the efflux ABC transporter systems also regulated in the Δphp1 mutant. However, the primary biological process populated by DGEs in Δltp1 involved genome stability. Comparison of the parental strain between the in vitro and in vivo condition indicated that DNA metabolic processes, including recombination and transposition, were significantly upregulated in vivo. Hence, Ltp1 specifically may be involved in the control of adaptation to in vivo conditions, while Ltp1 and Php1 have both common and enzyme-specific activities.

2025-08-20 | GSE299059 | GEO

Characterization of a bacterial tyrosine kinase in Porphyromonas gingivalis involved in polymicrobial synergy

Project description:RNA-Seq of wild-type Porphyromonas gingivalis compared to ΔPGN_1524 mutant Illumina based RNA-Seq was used to probe transcriptome differences between wild-type Porphyromonas gingivalis and ΔPGN_1524 mutant

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Characterization of a bacterial tyrosine kinase in Porphyromonas gingivalis involved in polymicrobial synergy

Project description:RNA-Seq of wild-type Porphyromonas gingivalis compared to ΔPGN_1524 mutant

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Role of PG1626 in Porphyromonas gingivalis ATCC33277

Project description:Wild type Porphyromonas gingivalis strain ATCC33277 (V3176) and PG1626 - deficient mutant (V3177) were grown in iron replete conditions was used to compare to Porphyromonas gingivalis strains grown in iron chelated conditions.

2023-06-08 | GSE174493 | GEO

Role of RNA-binding protein, Pgr, in Porphyromonas gingivalis

Project description:Role of RNA-binding protein, Pgr, in Porphyromonas gingivalis.

2022-10-08 | GSE168570 | GEO

Porphyromonas gingivalis gene expression during co-culture with Treponema denticola

Project description:Porphyromonas gingivalis and Treponema denticola are periodontalpathogens that are associated with the severity and progression of periodontal diseases. This study investigates the gene expression of Porphyromonas gingivalis during co-culture with Treponema denticola

2014-03-31 | E-MTAB-2214 | biostudies-arrayexpress

Treponema denticola gene expression during co-culture with Porphyromonas gingivalis

Project description:Porphyromonas gingivalis and Treponema denticola are periodontalpathogens that are associated with the severity and progression of periodontal diseases. this study investigates the gene expression of Treponema denticola during co-culture with Porphyromonas gingivalis.

2014-03-31 | E-MTAB-2257 | biostudies-arrayexpress

Porphyromonas gingivalis 33277 wild-type vs Fur orthologue Har isogenic mutant ECR455

Project description:Porphyromonas gingivalis is a major pathogen associated with the microbial biofilm-mediated disease chronic periodontitis. P. gingivalis has an obligate requirement for iron and protoporphyrin IX which it satisfies by transporting heme and iron liberated from the human host. The level of cellular iron in P. gingivalis affects the expression of a distinct iron-associated regulon of 64 genes and low iron invokes an iron sparing response. Iron homeostasis is usually mediated in Gram-negative bacteria at the transcriptional level by the Ferric Uptake Regulator (Fur). There is a single predicted P. gingivalis Fur superfamily orthologue named Har (heme associated regulator) that lacks the conserved metal binding residues found in other Fur orthologues. We show that Har binds both heme and ferrous iron resulting in a conformational change in the protein. Har was unable to complement the Escherichia coli H1780 fur mutant and there was no change in cellular metal content in a P. gingivalis Har mutant compared with the wild-type. The Har regulon of 44 genes is not predicted to play a role in iron homeostasis. Together these data indicated that Har does not regulate iron homeostasis in P. gingivalis. However, Har was required for heme-responsive biofilm development and its regulon overlapped P. gingivalis regulons previously identified after growth in heme limitation or as a homotypic biofilm. P. gingivalis is unique as an iron-dependent Gram-negative bacterium with a single heme-binding Fur superfamily orthologue, Har, that does not regulate iron homeostasis.

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