Project description:Interventions: Case series:Bifico
Primary outcome(s): Abundance of Clostridium Symbiosum;Abundance of Fusobacterium Nucleatum;survival time
Study Design: Case series
Project description:Whole genome bisulfite sequencing was performed on Chromomethylase-2 and Dicer-like-3 knockout mutants in order to confirm the results from genome wide association mapping and to identify the respective genomic regions that they target. Bisulfite sequencing of knockout mutants and WT controls
Project description:Illumina-based whole genome bisulfite sequencing libraries for 4 RNA-directed DNA methylation mutants Examining DNA methylation levels in leaf tissue of RNA-directed DNA methylation mutants
Project description:Using whole genome bisulfite sequencing to provide single-base resulution of DNA methylation status in ros1, idm1, and rdd mutants and identify hypermethylated regions comparing to wild type Arabidopsis plants. Examination of 3 different mutants' methylome. 8 samples (3 mutants, 1 WT, each has two biological replicates)
Project description:We treated C. albicans reference strain SC5314 with 20ng/ml of aureobasidin A, and obtained two mutants which were resistant to aureobasidin A. Then we did whole genome sequencing of these two mutants.
Project description:We found that several deacetylase-dead HDAC3 mutants were able to rescue the metabolic phenotype of HDAC3-depleted livers. Here we profile the histone acetylation in the presence of different HDAC3 mutants in mouse liver. Deacetylase-dead HDAC3 mutants, including HAHA, KA, YF and HEBI, were introduced into HDAC3-depleted (Cre) mouse livers by virus along with wild-type (WT) HDAC3 as a control. Livers were harvested at 5 pm (ZT 10) and subjected to ChIP with anti-H3K9ac antibodies followed by deep sequencing.
