Project description:In this study, we characterized the fatty acid production in Neochloris aquatica at transcriptomics and biochemical levels under limiting, normal, and excess nitrate concentrations in different growth phases. At the stationary phase, N. aquatica mainly produced saturated fatty acids such as stearic acid under the limiting nitrate concentration, which is suitable for biodiesel production. However, it produced polyunsaturated fatty acids such as α-linolenic acid under the excess nitrate concentration, which has nutritional values as food supplements. In addition, RNA-seq was employed to identify genes and pathways that were being affected in N. aquatica for three growth phases in the presence of the different nitrate amounts. Genes that are responsible for the production of saturated fatty acids were upregulated in the cells grown under a limiting nitrogen amount while genes that are responsible for the production of polyunsaturated fatty acid were upregulated in the cells grown under excess nitrogen amount. Further analysis showed more genes differentially expressed (DEGs) at the loga- rithmic phase in all conditions while a relatively steady trend was observed during the transition from the logarithmic phase to the stationary phase under limiting and excess nitrogen. Our results provide a foundation for identifying developmentally important genes and understanding the biological processes in the different growth phases of the N. aquatica in terms of biomass and lipid production.

2022-03-27 | GSE163658 | GEO

Rat LAD IR study

Project description:Rats underwent surgery for LAD ligation for 30 min followed by reperfusion. Heart ventricles were collected 2d or 7d after reperfusion. Keywords: rat heart ventricles, LAD - left anterior descending coronary artery, IR - ischemia-reperfusion

2006-03-24 | GSE4105 | GEO

Albula goreensis isolate:SM-30Jul2019_1

Project description:Albula goreensis isolate:SM-30Jul2019_1 Genome sequencing and assembly

| PRJNA692825 | ENA

Expression data from CCR2+ and CCR2- splenic marophages from C57BL6 mouse infected with Listeria Monocytogenes in vivo on day3

Project description:We used microarrays to detail the global gene expression in CCR2+ and CCR2- spenic macrophages (SM) sorted from C57BL6 mouse infected with Listeria Monocytogenes in vivo on day3

2019-01-01 | GSE97790 | GEO

Buchnera aphidicola strain:Sm | isolate:Midelt

Project description:Buchnera aphidicola strain:Sm | isolate:Midelt Genome sequencing

| PRJNA797932 | ENA