Project description:Microarray analysis on human neuroblastoma cells exposed to iron, B-amyloid or the B-amyloid iron complex. Alzheimer's disease is associated with an abnormal accumulation of certain metal ions.
Project description:Microarray analysis of gene expression profiles in human neuroblastoma cells exposed to Amyloid beta-Zn and Amyloid beta-Cu complexes
Project description:A typical pathological feature of Alzheimer’s disease (AD) is the appearance in the brain of senile plaques made up of B-amyloid (AB) and neurofibrillary tangles. Ad is also associated with an abnormal accumulation of some metal ions and we have recently shown that one of these, aluminum (Al), plays a relevant role in affecting AB aggregation and neurotoxicity. In this study, employing a microarray analysis of 29,166 genes, we investigated the effects induced by the exposure to the AB-Al complex on the gene expression profile of a cell line of neuronal-like cells, the SH-SY5Y. Results from the microarray essay indicate that, compared to AB or Al alone, exposure to AB-Al produced selective changes in gene expression. Some of the genes selectively over or under expressed are directly related to AD.
Project description:A microarray-based transcriptome profiling was performed to provide a better understanding of the effects of maleic acid on human. Gene expression profiles of human neuroblastoma SH-SY5Y cells exposed to three concentrations of maleic acid (10, 50 and 100 μM) for 24 h were analyzed.
