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ABSTRACT: Carollia sowelli (Sowell's short-tailed bat) genome, mCarSow1, sequence data

PROVIDER: PRJNA1278911 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR34021887_subreads.fastq.gz	Fastqsanger.gz
	SRR34021889_subreads.fastq.gz	Fastqsanger.gz
	SRR34021890_subreads.fastq.gz	Fastqsanger.gz
	SRR34021891_subreads.fastq.gz	Fastqsanger.gz

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Project description:Carollia sowelli (Sowell's short-tailed bat) genome, mCarSow1

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Transcriptome comparison of bat (Miniopterus natalensis) forelimb and hindlimb autopods at developmental stages CS16 and CS17 and E13.5 mouse forelimb autopods

2014-02-15 | GSE51042 | GEO

Transcriptome comparison of bat (Miniopterus natalensis) forelimb and hindlimb autopods at developmental stages CS16 and CS17 and E13.5 mouse forelimb autopods

Project description:The bat offers an alternative paradigm to the standard mouse and chick model of limb development as it has extremely divergent forelimbs (long digits supporting a wing) and hindlimbs (short digits and claws) due the distinct requirements of both aerial and terrestrial locomotion. We used a cross-species microarray approach to identify differentially expressed (DE) genes between the bat (Minniopterus natalensis) forelimb and hindlimb autopods at Carollia developmental stages (CS) 16 and CS17, and between the bat (CS17) and mouse (E13.5) forelimb autopods. Several DE genes were identified, including two homeobox genes, Meis2, a proximal limb-patterning gene, and Hoxd11, a gene involved in digit elongation. Both genes are significantly over-expressed in the developing bat forelimb as compared to the hindlimb and equivalently staged mouse forelimbs. A reference design was used in this microarray experiment. A pool of left and right mouse forelimb autopods from 24 embryos was used as the reference sample. This sample was directly compared to individual CS16 and CS17 bat fore- and hindlimbs (left and right of one individual pooled) that were classified as the test conditions. Four experimental sessions were performed using an independently amplified mouse reference pool and 4 biological repeats for the bat limbs. These samples were co-hybridised to OPERON Mouse OpArray (ver. 4.0) spotted oligonucleotide slides to perform a competitive Cross-Species Hybridisation experiment. The bat aRNA (test) samples were labelled with Cy3 dye (green signal), the mouse aRNA (reference) sample was labelled with Cy5 dye (red signal).

2014-02-15 | E-GEOD-51042 | biostudies-arrayexpress

Project description:Transcriptomics of plagiopatagium formation in Seba's short-tailed bat

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Phoebastria albatrus

Project description:Draft genome sequence of Short-tailed albatross

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RNA-seq of 6 tissues from Monodelphis domestica to investigate the evolution of gene expression levels in mammalian organs

Project description:This experiment contains the subset of data corresponding to gray short-tailed opossum RNA-Seq data from experiment E-GEOD-30352 (http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-30352/), which goal is to understand the dynamics of mammalian transcriptome evolution. To study mammalian transcriptome evolution at high resolution, we generated RNA-Seq data (∼3.2 billion Illumina Genome Analyser IIx reads of 76 base pairs) for the polyadenylated RNA fraction of brain (cerebral cortex or whole brain without cerebellum), cerebellum, heart, kidney, liver and testis (usually from one male and one female per somatic tissue and two males for testis) from nine mammalian species: placental mammals (great apes, including humans; rhesus macaque; mouse), marsupials (gray short-tailed opossum) and monotremes (platypus). Corresponding data (∼0.3 billion reads) were generated for a bird (red jungle fowl, a non-domesticated chicken) and used as an evolutionary outgroup.

2011-10-12 | E-MTAB-3719 | biostudies-arrayexpress

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