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ABSTRACT: Diet of Copsychus saularis and Copsychus malabaricus in oil palm plantation

PROVIDER: PRJNA1278935 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR34027568_1.fastq.gz	Fastqsanger.gz
	SRR34027568_2.fastq.gz	Fastqsanger.gz
	SRR34027570_1.fastq.gz	Fastqsanger.gz
	SRR34027570_2.fastq.gz	Fastqsanger.gz

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Project description:Diet of Copsychus saularis and Copsychus malabaricus

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Project description:Oil palm breeding and seed development have been hindered due to the male parent's incapacity to produce male inflorescence as a source of pollen under normal conditions. On the other hand, a young oil palm plantation has a low pollination rate due to a lack of male flowers. These are the common problem of sex ratio in the oil palm industry. Nevertheless, the regulation of sex ratio in oil palm plants is a complex mechanism and remains an open question until now. Researchers have previously used complete defoliation to induce male inflorescences, but the biological and molecular mechanisms underlying this morphological change have yet to be discovered. Here, we present an RNA-seq dataset from three early stages of an oil palm inflorescence under normal conditions and complete defoliation stress. This transcriptomic dataset is a valuable resource to improve our understanding of sex determination mechanisms in oil palm inflorescence.

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Project description:C57Bl/6J mice were put on a low fat diet (10 kcal% palm oil) or high fat diet (60 kcal% palm oil), combined with a low dietary Mg2+ (0.03 w/w% or 0.21 w/w%) for a period of 17 weeks. White adipose tissue was harvested and total RNA isolated using TRIzol, of which 5 samples per group were sent to BGI-China for RNA-Sequencing.

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Palm oil-rich diet affects murine liver proteome and S-palmitoylome

Project description:Palmitic acid (C16:0) is the most abundant saturated fatty acid in animals serving as a substrate in lipid synthesis and β-oxidation, and in the modification of proteins called palmitoylation. The influence of dietary palmitic acid on protein palmitoylation in the context of metabolic processes remains largely unknown. In this study we performed high-throughput proteomic analyses of the liver membrane fraction in the mouse to examine the influence of a palm oil-rich diet on the level and S-palmitoylation of proteins. For this purpose, mice were fed for 4 or 12 weeks a diet containing 19.1% of palm oil in addition to 4% soybean oil (45% kcal from fat) while 4% soybean oil (10% kcal from fat) was the only fat source in the control diet. Liver functioning and pro-inflammatory responses of the liver and peritoneal macrophages as well as the input of protein S-palmitoylation to these aspects were assessed in parallel. We found that the diet rich in palm oil induced transient accumulation of C16:0 and C18:1 fatty acids in murine liver leading to changes of the level and S-palmitoylation of numerous proteins involved in liver metabolism and selected innate immune responses. The relatively mild negative impact of such diet on liver functioning can be attributed to a lower bioavailability of palm oil-derived C16:0 vs. that of C18:1 and the efficiency of mechanisms preventing liver injury, including dynamic protein S-palmitoylation.

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