Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1280742 | ENA |
REPOSITORIES: ENA
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Project description:We tested the transcriptome of embryos from WT and Dux KO mice including zygotes (hCG 28h), early 2-cell (hCG 31-32h), middle 2-cell(hCG 41-42h), late 2-cell(hCG 47-48h) and, Dux mRNA injected embryos including zygote(5h after injection, hCG 28h), early 4-cell(6h after injection, hCG 54h) and late 4-cell (17h after injection, hCG 65h) using the Covaris DNA shearing protocol for Smart-seq sequence library generation. We found that the activation of Dux is important but not essential for ZGA, but the silencing and elimination of Dux is strictly necessary for early embryonic development.
2019-09-24 | GSE132045 | GEO
Project description:To further development of the effects of β-HCG in ovarian cancer SKOV3 cells，we have employed lncRNA and mRNA microarray as a discovery platform to identify lncRNA and mRNA expression in β-HCG overexpressing ovarian cancer cells. SKOV3 cells were transfected with lentiviral vector with eGFP, encoding β-HCG and negative control vector (LV- β-HCG and LV-CON,) by using polybrene. The dysregulation of β-HCG was confirmed by using RT-PCR. RNA was extracted and detected by a lncRNA and mRNA microarray in LV-β-HCG and LV-CON SKOV3 cells. The different expression of lncRNA and mRNA in LV-β-HCG and LV-CON SKOV3 cells was analyzed to explore the mechanism that β-HCG affect ovarain cancer cells.
2018-11-06 | GSE122147 | GEO
Project description:Neurotensin (NTS) is a small 13 amino acid neuropeptide. In the mouse ovary, the expression of Nts ovary increases 250-fold 4h after hCG. Similarly, in granulosa cells, the mRNA levels of Nts increased rapidly at 4h after hCG stimulation. Interestingly, the Nts mRNA levels in granulosa cells were approximately 8-fold higher at 4 h after hCG than in whole ovaries. However, the potential mechanisms of NTS action in the ovulatory process are unknown. The present study determined the regulatory mechanisms driving the expression of Nts and functions of the NTS using primary mouse granulosa cells. hCG activated PKA and p38MAPK signaling pathways, and inhibitors for these pathways abolished hCG-induced increases in the levels of Nts. To identify the genes regulated by NTS, high throughput RNA sequencing was performed using Nts silenced mouse granulosa cells treated with or without hCG. Sequencing data analysis revealed that Nts knockdown affects the expression of several genes that were not previously identified in the ovary. qPCR analysis verified hCG-induced, Nts-regulated expression of a selection of these genes. This study revealed novel genes regulated by NTS, thereby providing a function for NTS in the ovulatory process.
2022-10-03 | GSE210811 | GEO
Project description:We sought to identify genes that are regulated by the ovulatory signals in mouse cumulus cells. We compared the transcriptomes between cumulus cells colected from mice before (hCG-0h) and after 6 h (hCG-6h) of hCG injection.
2016-08-01 | GSE80255 | GEO
Project description:Chromatin immunoprecipitation-based sequencing (ChIP-seq) of H3K27Ac was performed using mouse ovarian granulosa cells before and after ovulatory signal stimulation. We found genome-wide H3K27Ac was erased rapidly at 1h after hCG treatment, and new H3K27Ac was established at 4h after hCG treatment.
2021-01-30 | GSE165809 | GEO
Project description:lncRAN and mRNA expression signatures in SKOV3 cells: control vs. β-HCG transfected
| PRJNA503830 | ENA
Project description:The LH surge triggers dramatic transcriptional changes in genes associated with ovulation and luteinization. The present study investigated the spatiotemporal expression of nuclear factor interleukin-3 (NFIL3), a transcriptional regulator of the bZIP transcription factor superfamily, and its potential role in the ovary during the periovulatory period. NFIL3, also known as E4-binding protein 4 or NFIL3/E4BP4, was originally identified as a transcriptional repressor based on its DNA-binding activity at the promoter of the gene encoding the adenovirus E4 protein. Immature female rats were injected with PMSG, treated with hCG, and ovaries or granulosa cells were collected at various times after hCG. Nfil3 mRNA was highly induced both in intact ovaries and granulosa cells after hCG treatment. In situ hybridization demonstrated that Nfil3 mRNA was highly induced in theca-interstitial cells at 4-8 h after hCG, localized to granulosa cells at 12 h, and decreased at 24 h. Over-expression of NFIL3 in granulosa cells inhibited the induction of prostaglandin-endoperoxide synthase 2 (Ptgs2), progesterone receptor (Pgr), epiregulin (Ereg), and amphiregulin (Areg) and down regulated levels of prostaglandin E2. The inhibitory effect on Ptgs2 induction was reversed by NFIL3 siRNA treatment. In theca-interstitial cells the expression of hydroxyprostaglandin dehydrogenase 15-(NAD) (Hpgd) was also inhibited by NFIL3 over-expression. Data from luciferase assays demonstrated that NFIL3 over-expression decreased the induction of the Ptgs2 and Areg promoter activity. EMSA and ChIP analyses indicated that NFIL3 binds to the promoter region containing the DNA binding sites of CREB and C/EBP?. In summary, hCG induction of NFIL3 expression may modulate the process of ovulation and theca-interstitial and granulosa cell differentiation by regulating expression of PTGS2, PGR, AREG, EREG, and HPGD, potentially through interactions with CREB and C/EBP? on their target gene promoters.
2011-01-20 | GSE26730 | GEO
Project description:We sought to identify genes that are regulated by the ovulatory signals in mouse cumulus cells. We compared the transcriptomes between cumulus cells colected from mice before (hCG-0h) and after 6 h (hCG-6h) of hCG injection. 3 individual cumulus cell samples of hCG-0h and hCG-6h were collected and subjected to microarray study. Copmarison was done between hCG-0h and hCG-6h groups.
2016-08-01 | E-GEOD-80255 | biostudies-arrayexpress
Project description:The first is transcriptional profiling of siRNA-mediated knockdown of IQGAP3 in human gastric cancer cell line AGS, NUGC3, and Hs746T comparing with non-target siRNA transfected AGS, NUGC3, and Hs746T as a control. The aim of this experiment was to evaluate the functions of IQGAP3 in three different gastric cancer cell lines in vitro. The second is bulk RNA-sequence from subcutaneous tumors, which were injected NUGC3 shIQGAP3 knockdown cells and NUGC shContorl cells into immunodeficient mice (NSG mice). The aim is to evaluate the difference of transcriptional profiling in human cancer cells and mouse stromal cells of shIQGAP3 knockdown tumors compared to shContorol tumors.
2025-03-12 | GSE231642 | GEO