Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1281020 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Microbial extracellular electron uptake (EEU) is central to bioelectrochemical processes and biocorrosion, yet its molecular mechanisms remain incompletely understood. Here, we investigate how excess Fe2+ modulates EEU in Desulfovibrio ferrophilus IS5, a strain that causes severe anaerobic iron corrosion via outer-membrane cytochromes (OMCs)-mediated electron uptake. We show that IS5 grown with elevated Fe2+ exhibits substantially enhanced EEU. This enhancement arises through two complementary mechanisms: (i) increased abundance of functional OMCs via upregulation of a cytochrome assembly protein, and (ii) an additional electron transfer route mediated by FeS nanoparticles precipitated on the IS5 outer membrane. Remarkably, IS5 with low OMCs expression but biosynthesized FeS can rapidly shift to EEU before OMCs induction. These findings suggest that during iron corrosion, when IS5 cells are embedded within thick corrosion crusts and biofilms and face both high Fe2+ concentrations and organic limitation, they exploit OMCs and FeS nanoparticles in parallel to sustain high-rate EEU from iron. This study advances the mechanistic understanding of EEU-driven iron corrosion and highlights a potential avenue for manipulating bioelectrochemical systems.
2026-03-24 | GSE313310 | GEO
Project description:Geobacter species are of great interest for environmental and biotechnology applications as they can carry out direct electron transfer to insoluble metals or other microorganisms and have the ability to assimilate inorganic carbon. Here, we report on the capability and key enabling metabolic machinery of Geobacter metallireducens GS-15 to carry out CO2 fixation and direct electron transfer to iron. An updated metabolic reconstruction was generated, growth screens on targeted conditions of interest were performed, and constraint-based analysis was utilized to characterize and evaluate critical pathways and reactions in G. metallireducens. The novel capability of G. metallireducens to grow autotrophically with formate and Fe(III) was predicted and subsequently validated in vivo. Additionally, the energetic cost of transferring electrons to an external electron acceptor was determined through analysis of growth experiments carried out using three different electron acceptors (Fe(III), nitrate, and fumarate) by systematically isolating and examining different parts of the electron transport chain. The updated reconstruction will serve as a knowledgebase for understanding and engineering Geobacter and similar species.
2023-11-27 | MODEL2204190002 | BioModels
Project description:Differential expression of electron transfer genes during growth with insoluble iron provided as an electron acceptor compared to soluble iron.
2014-10-01 | GSE59467 | GEO
Project description:Differential expression of electron transfer genes during growth with insoluble iron provided as an electron acceptor compared to soluble iron. A four chip study using total RNA recovered from two separate cultures of Ferroglobus placidus DSM 10642 grown with 10 mM acetate provided as electron donor and insoluble iron hydroxide provided as electron acceptor (experimental condition) and two separate cultures of Ferroglobus placidus DSM 10642 grown on 10 mM acetate with soluble iron citrate provided as electron acceptor (control condition). Each chip measures the expression level of 2613 genes from Ferroglobus placidus DSM 10642 with nine 45-60-mer probe pairs (PM/MM) per gene, with three-fold technical redundancy.
2014-10-01 | E-GEOD-59467 | biostudies-arrayexpress