Project description:We employed the Chromium GEM-X single-cell RNA sequencing platform by 10x Genomics to investigate how the removal of p16-high-expressing cells affects the lung single-cell transcriptome of 22-month-old male mice. In this experiment, we utilized p16-CreERT2/R26-LSL-DTR mice, administering tamoxifen and diphtheria toxin at 21 months of age. Lung tissues were harvested two weeks later for analysis. Our objective was to examine how the elimination of p16-high cells influences intercellular interactions among diverse cell types in aged lungs.
Project description:We employed the Chromium GEM-X single-cell RNA sequencing platform by 10x Genomics to investigate the effects of the GLS1 inhibitor, BPTES, on the lung single-cell transcriptome of 19- to 20-month-old wild-type male mice. Previous studies have identified GLS1 as a potential senolytic reagent capable of eliminating senescent cells. Our aim was to determine which cell populations are selectively removed by GLS1 inhibition in aged mice and to assess how this intervention alters intercellular interactions across different cell types.
Project description:We used the scRNA-seq to establish a comprehensive molecular cell atlas of the healthy canine lung, expanding our knowledge of lung cell subpopulations in dogs, and providing the molecular foundation for investigating lung cell identities and functions in lung diseases across species.
Project description:To investigate the heterogeneity of lung CD140a+ mesenchymal cells (fibroblasts) and identify the specific lung fibroblast subset, we purified CD140a+ lung cells using CD140a-EGFP reporter mice for a higher-resolution scRNA-seq.
Project description:To investigate the heterogeneity of lung stromal cells and identify the specific lung stromal subset, we performed single cell RNA-sequencing (scRNA-seq) on lung stromal cells (CD45-CD31-CD326-). Around 6800 cells were captured using the 10x Chromium technology.
Project description:To investigate the CD140a+ mesenchymal cell heterogeneity in the lung in both steady and tumor-bearing conditions, we utilized CD140aEGFP reporter mice, and purified CD140a+ lung mesenchymal cells from naive and tumor-bearing mice and performed scRNA-seq.