Project description:RNA samples from chicken (Gallus gallus) embryonic fibroblasts (sample ID: ES90) as well as from the HD11 chicken macrophage cell line (sample ID: ES91) were sequenced at the National Institutes of Health Intramural Sequencing Center (NISC) using the Illumina GAIIx technology running the standard CASAVA/ELAND pipeline.
Project description:We used LPS-stimulated chicken HD11 macrophage-like cell as a model to identify the key transcription factors involved in transcriptome regulation responsible for SeMC treatment. RNA-seq identified 3,263 transcripts significantly differentially expressed between the SeMC treated group and the control group, and 1,344 transcripts significantly differentially expressed between LPS+SeMC and LPS treated group (FDR < 0.05, FDR > 1.5). Bioinformatic analysis revealed that six transcription factors, NFKB2, RFX2, E2F5, ETV5, BACH1, and E2F7 were candidate genes for transcriptome regulation in SeMC treated HD11 cells.
Project description:We used LPS-stimulated chicken HD11 macrophage-like cell as a model to identify the key transcription factors involved in transcriptome regulation responsible for Procyanidin B1 treatment. RNA-seq identified 1,384 transcripts significantly differentially expressed between the Procyanidin B1 treated group and the control group, and 696 transcripts significantly differentially expressed between LPS+Procyanidin B1 and LPS treated group (FDR < 0.05, FDR > 1.5). Bioinformatic analysis revealed that five transcription factors, FOSL1::JUND, CREB1, ZNF467, STAT2, and HIF1A were candidate genes for transcriptome regulation in Procyanidin B1 treated HD11 cells.
Project description:HD11 cells were stimulated with 1 ug/ml endotoxin from ST-798 for 1, 2, 4 and 8 hours Cells were harvested at 1, 2, 4 and 8 hrs post stimulation and RNA was isolated from these cells and microarray was conducted for these RNA samples using affymetrix genechips
2010-10-19 | GSE23881 | GEO
Project description:Lipopolysaccharide effect in HD11 cells
| PRJNA1153035 | ENA
Project description:Postbiotic - LPS interaction in HD11 cells
Project description:The scavenger receptor MARCO mediate double-stranded RNA (dsRNA) uptake through binding to viral dsRNA at the cell surface and to enable this dsRNA to interact with the important dsRNA recognition receptor TLR3 in the endosome. To investigate the antiviral function of MARCO in ALV-J infection, we knocked out the MARCO gene in HD11 cells using the CRISPR/Cas9 technique and then performed RNA-sequencing on wild-type and mutant macrophages. Under the presence of MARCO, macrophages response to viral infection through inducing TLR3-IL-1β dependent inflammatory response. Conversely, lack of MARCO increased the viral replication levels and attenuated the antiviral inflammatory response.
Project description:Chicken endogenous retroviruses (chERVs) are remnants of ancient retroviral infections and can profoundly affect the host antiviral innate immune response, although the mechanisms by which these changes occur are largely unknown. To investigate the antiviral function of chERVs in ALV-J infection, we knocked down the expression of chERVs in HD11 cells using the CRISPR/Cas9 technique and then performed RNA-sequencing on wild-type and mutant macrophages. Under the presence of chERVs, macrophages response to viral infection through MARCO-TLR3-IL-1β dependent inflammatory response. Conversely, knowndown of chERVs increased the viral replication levels and attenuated the antiviral inflammatory response.
Project description:We infected HD11 macrophages with Salmonella Typhimurium, followed by chemical proteomic analysis of deubiquitinases by using ubiquitin-specific active-site probe.
