Project description:Like many neurological disorders, Alzheimer’s disease has a sex-biased epidemiological profile, affecting approximately twice as many women as men. The cause of this sex difference has yet to be elucidated. To identify molecular correlates of this sex bias, we investigated molecular pathology in females and males using the 5XFAD genetic mouse model of Alzheimer’s disease. We profiled the transcriptome and proteome of the mouse hippocampus during early stages of disease development (1, 2, and 4 months) with RNA-sequencing and Liquid-chromatography mass spectrometry.
2018-10-10 | PXD006587 | Pride
Project description:RNA-seq analysis of mouse hippocampus
Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived hippocampus transcriptome profiling (RNA-seq) in mouse to evaluate alterations in hippocampus with methamphetamine administrations.
Project description:Inbred mouse strains differ in their innate anxiety levels. We used RNA-sequencing to identify gene expression differences in hippocampus and frontal cortex of six mouse strains.
Project description:CWG-cPIP targets CAG/CTG (CWG) triplet repeat DNA to inhibit the transcription in a repeat length-dependent manner. To investigate off-target effects of CWG-cPIP on gene expression in the brain, CWG-cPIP (83 ug/kg) was injected into mouse hippocampus. RNA sequencing analysis was performed using vehicle- or CWG-cPIP-injected mouse hippocampus 3 weeks later, mixing with ERCC RNA spike-in controls.
Project description:Antisense and sense probes of noncoding RNA GAS5 were used to perform the RNA pull down experiment on mouse hippocampus tissues. Then the proteins were identified by LC-MS/MS.
2021-09-09 | PXD023363 | Pride
Project description:RNA-Seq of Men1-KO mouse hippocampus
