Project description:Transcriptional profiling of liver tissue from rats fed with standard diet with 1% cholesterol (control diet) and the same diet supplemented with 5% Geneva (test sample1) or 5% Hayward (test sample2) kiwifruit lyophylisate. The goal was to determine the anti-atherogenic effect of kiwi fruit supplementation and their impact on gene expression preventing hepatic steatosis
Project description:We explore whether a low-energy diet intervention for Metabolic dysfunction-associated steatohepatitis (MASH) improves liver disease by means of modulating the gut microbiome. 16 individuals were given a low-energy diet (880 kcal, consisting of bars, soups, and shakes) for 12 weeks, followed by a stepped re-introduction to whole for an additional 12 weeks. Stool samples were obtained at 0, 12, and 24 weeks for microbiome analysis. Fecal microbiome were measured using 16S rRNA gene sequencing. Positive control (Zymo DNA standard D6305) and negative control (PBS extraction) were included in the sequencing. We found that low-energy diet improved MASH disease without lasting alterations to the gut microbiome.
Project description:Plant-based diets rich in fruit and vegetables can prevent development of several chronic age-related diseases such as cancer and cardiovascular diseases. The mechanisms behind this protective effect is not elucidated. We have studied whether a specially designed antioxidant-rich food diet and a kiwi-fruit intervention can affect whole genome expression in human blood cells with emphasis on stress and repair related process.
Project description:Using ChIP-seq to study the deposition of RIN in WT tomato AC fruits and pCBC::RIN-3xFLAG, pCBC::RIN-3xFLAG/dml2-3 tagline fruits at 34 dpa (Breaker stage)
