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ScRNAseq and TCRseq of tumor infiltrating CD8 T cells from samples

ABSTRACT: scRNAseq and TCRseq of tumor infiltrating CD8 T cells from samples

PROVIDER: PRJNA1289439 | ENA |

REPOSITORIES: ENA

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scRNAseq and TCRseq of tumor infiltrating CD8 T cells from samples

Project description:The goal of this study is to investigate the role of ageing on tumor infiltrating CD8 T cells Ageing is a major risk factor for various cancers, and patients aged 65 years or older represent up to 60% of newly diagnosed cancer patients. Ageing results in remodeling of T cell immunity, causing poor clinical outcomes in patients with cancer. During ageing, T cells undergo age-dependent changes and gradually lose their physiological function. Numerous studies have revealed that T cells mediate the surveillance and elimination of tumors, and substantial therapeutic strategies have been developed to improve tumor-specific T cell responses. However, these experimental studies were almost exclusively conducted in young but not old individuals. Therefore, how age-associated T cell dysfunction impacts antitumor immunity requires further investigation.

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scRNAseq and scTCRseq data from tumor-infiltrating lymphocytes

Project description:scRNAseq and scTCRseq data from tumor-infiltrating lymphocytes

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Expression data from FACS-sorted CD8+BTLA+ vs CD8+BTLA- Human Tumor-infiltrating Lymphocytes in Metastatic Melanoma

Project description:Adoptive T-cell Therapy (ACT) involves using tumor-infiltrating lymphocytes (TIL) isolated from metastatic melanoma and expanding them ex vivo prior to infusion into lympho-depleted patients. This is one of the most promising approaches to treat metastatic melanoma, with the rates of clinical response between 48-50% based on studies done at NCI, M.D. Anderson Cancer Center (Houston, TX), and Sheba Medical Center (Tel Aviv, Israel). In the Phase II ACT Trial at M.D. Anderson Cancer Center , our group has uncovered an association between positive clinical response and the amount of CD8+ tumor-infiltrating lymphocytes expressing B and T Lymphocyte Attenuator (BTLA), a reported inhibitory receptor on T-cells. We used microarrays to detail the differences in the global programme of gene expression between CD8+BTLA+ vs CD8+BTLA- TILs in order to understand the molecular basis of the clinical association. TILs were isolated by enzymatically digest the melanoma tumor fragments obtained from Stage IIIc/IV melanoma patients at M.D. Anderson Cancer Center. The TILs were expanded with high-dose IL-2 for two weeks prior to sorting by FACS (fluoresecence-activated cell sorter) for CD8+BTLA+ and CD8+BTLA- susbets. RNA was extracted from each sorted subsets and hybridized on Affymetrix microarrays

2013-01-03 | E-GEOD-43260 | biostudies-arrayexpress

scRNAseq and scTCRseq data from tumor-infiltrating lymphocytes

Project description:In cancer models, CD103+CD8 TRM cells are implicated in the response to anti-PD-1 immunotherapy, and a subset of CD4 TRM cells expressing the integrin CD49a is required for response to anti-CTLA-4 immunotherapy. We performed single-cell RNA sequencing on tumour-infiltrating T lymphocytes (TILs) isolated from colon carcinoma et unsupervised analyses reveal several TRM populations.

2025-05-27 | GSE298152 | GEO

Adoptive Cell Therapy Using Cancer Specific CD8+ Tumor Infiltrating Lymphocytes in Adult Patients With Solid Tumors

Project description:The subject of this study is the adoptive transfer of selected autologous tumor infiltrating lymphocytes (TIL) after in vitro expansion for the treatment of solid tumor malignancies. The TIL selection process is based on evidence showing that CD8+ TIL which co-express both CD39 and CD103 harbor the bulk of tumor-reactivity and that the remaining CD8 TIL is mainly composed of non-tumor reactive bystander cells. All of the expanded TIL that are produced (1-40 billion are expected) will be delivered in the form of a cell suspension to the participants by intravenous infusion. It is proposed that these selected TIL will produce a more potent and efficacious treatment of late-stage cancer.

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Project description:To investigate the function of LRIG11 in regulating the differentiation of polyclonal CD8 tumor infiltrating T cells during the response to B16melanoma. And, To investigate the function of LRIG1 in regulating the activaiton of ovalbumin specific OT1 CD8+ T cells during the response to B16 tumor stably expresss ovalbumin as surrogate tumor antigen.

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