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Gene expression effects of KDAC6 (HDAC6) knockout in HT1080 cells

ABSTRACT: Gene expression effects of KDAC6 (HDAC6) knockout in HT1080 cells

PROVIDER: PRJNA1290097 | ENA |

REPOSITORIES: ENA

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Gene expression effects of KDAC6 (HDAC6) knockout in HT1080 cells

Project description:To study the gene expression and phenotype roles of KDAC6, CRISPR/Cas9 was used to create knockout cell lines from wild-type HT1080 cells. Four individual isolated knockout clones were pooled to create the line. Comparable wild-type data is available in GSE302260.

2025-08-10 | GSE302329 | GEO

Gene expression profile changes in HDAC6 knockdown HT1080 cells cells after TLR3 stimulation

Project description:TLR3 stimulation by extracellular dsRNA (e.g. polyIC) induces expression of numerous genes. The knockdown of HDAC6 prior to TLR3 stimulation leads to an ablation of expression of IRF-3-dependent genes. Total RNA extracted from HT1080 cells expressing control-shRNA or HDAC6-shRNA, after 6 h of treatment with dsRNA (polyIC), compared to untreated controls

2013-12-31 | E-GEOD-43218 | biostudies-arrayexpress

Gene expression effects of KDAC8 (HDAC8) knockout in HT1080 cells

Project description:Gene expression effects of KDAC8 (HDAC8) knockout in HT1080 cells

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Gene expression effects of KDAC8 (HDAC8) knockout in HT1080 cells

Project description:To study the gene expression and phenotype roles of KDAC8, CRISPR/Cas9 was used to create knockout cell lines from wild-type HT1080 cells. Four individual isolated knockout clones were pooled to create the line. Comparable wild-type data is available in GSE302260.

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Evaluation of KDAC6 (HDAC6) inhibitor selectivity in HT1080 cells

Project description:Evaluation of KDAC6 (HDAC6) inhibitor selectivity in HT1080 cells

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Evaluation of KDAC6 (HDAC6) inhibitor selectivity in HT1080 cells

Project description:To investigate the specificity and biological impacts of KDAC-selective inhibitors, we treated wild-type HT1080 cells with KDAC6 selective inhibitors SW-113 and SE-138. Expression profiling analysis was then performed on the treated cells vs. untreated wild-type HT1080 cells. Comparable wild-type data is available in GSE302260.

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Expression data of liver samples of dex or vehicle treated wildtype and HDAC6- knockout C57Bl/6 mice respectively

Project description:In the present study, we investigated the importance of histone deacetylase 6 (HDAC6) for glucocorticoid receptor (GR) mediated effects on glucose metabolism, and its potential as a therapeutic target for the prevention of glucocorticoid (GC)-induced diabetes. Dexamethasone (dex)-induced hepatic glucose output and GR translocation were analysed in wildtype (wt) and HDAC6-deficient (HDAC6ko) mice. The effect of the specific HDAC6-inhibitor tubacin was analysed in-vitro. Wt and HDAC6ko mice were subjected to 3 weeks dex treatment before analysis of glucose and insulin tolerance. HDAC6ko mice showed impaired dex-induced hepatic GR translocation. Accordingly, dex induced expression of a large number of hepatic genes was significantly attenuated in mice lacking HDAC6 and by tubacin in-vitro. Glucose output of primary hepatocytes from HDAC6ko mice was diminished. A significant improvement of dex-induced whole-body glucose intolerance as well as insulin resistance in HDAC6ko mice compared to wt littermates was observed. The present study demonstrates that HDAC6 is an essential regulator of hepatic GC stimulated gluconeogenesis and impairment of whole body glucose metabolism through modification of GR nuclear translocation. Selective pharmacological inhibition of HDAC6 may provide a future therapeutic option against the pro-diabetogenic actions of GCs. In this dataset, we include the expression data obtained from isolated RNA of dissected mouse livers using wildtype and HDAC6 deficient animals which were treated over a timespan of 3 weeks with 1mg/kg dexamethasone and vehicle respectively. These data are used to show the hdac6-deficiency mediated attenuation of several dexamethasone induced genes. 12 samples in total were analyzed. 3 samples of different animals of each group (wt vehicle, wt dexamethasone, hdac6ko vehicle and hdac6ko dexamethasone)

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HDAC6 and HSP90 control the functions of Foxp3+ T regulatory cells

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Effects of IFN beta treatment on gene expression in human fibrosarcoma HT1080 cells

Project description:Effects of IFN beta treatment on gene expression in human fibrosarcoma HT1080 cells

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