Project description:<p>This dataset contains raw liquid chromatography-tandem mass spectrometry (LC-MS/MS) data derived from the liver tissues of goats (Capra hircus). The purpose of this study is to explore the metabolic regulatory mechanism of the probiotic Enterococcus faecalis JM263 in goats fed a high-concentrate diet (HCD).</p><p>Experimental Design</p><p>Feeding experiments were conducted on yaks, divided into two groups: 1) Control group, fed a high-concentrate diet; 2) Treatment group, fed the same HCD supplemented with Enterococcus faecalis JM263. After intervention, liver tissues were collected, and metabolomic analysis was performed following quenching and extraction.</p><p>Objectives</p><p>Untargeted metabolomics was used to identify differential metabolites and enriched pathways related to the hepatoprotective effect of the probiotic. This dataset supports the findings regarding the gut-liver axis interaction in ruminants under dietary stress.</p>
Project description:<p>This study adopted the MRM wide-targeted metabolomics approach to detect metabolites in herbal plant samples. Using ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry (QTrap 6500+), combined with specific extraction and separation methods, qualitative and quantitative analysis of multiple metabolites in plant samples was achieved, providing data support for the study of metabolic characteristics of herbal plants.</p>
Project description:Asterids is one of the major plant clades comprising of many commercially important medicinal species. One of the major concerns in medicinal plant industry is adulteration/contamination resulting from misidentification of herbal plants. This study reports the construction and validation of a microarray capable of fingerprinting medicinally important species from the Asterids clade.
Project description:Rumen epithelial parakeratosis, a common disease in ruminants caused by abnormalities in the ruminal stratified squamous epithelial keratinization process, negatively impacts ruminant health and performance. While we still lack a comprehensive perception of the underlying mechanisms and the predisposing factors for this disorder.Here, we investigated rumen epithelial cell heterogeneity, differentiation trajectories, and cornification to clarify the rumen epithelial keratinization process
Project description:Stresses from either biotic or abiotic origins can have significant impact towards plant physiology and molecular regulation. Jasmonate acid (JA) and its derivative, methyl JA (MeJA) are hormonal cues released by plants which signal defensive response to curb the damage from such stresses. In an attempt to study the defensive response, a tropical herbal plant, Persicaria minor (P. minor) which is known for its pungent smell as well as various bioactivities including antimicrobial and anti-cancer, has been treated with MeJA to invoke the stress signaling. Such elicitation has been performed in various plants such as Arabidopsis, rice and hairy root cultures of certain herbs, yet how MeJA directly influenced the proteome of a herbal species particularly P. minor has not been previously elucidated. In this study, P. minor plants was exogenously treated with MeJA and its proteome was investigated using a new proteomics approach called SWATH-MS.
Project description:To investigate the evolutionary changes of regulatory elements in ruminants, we then performed regulatory elements profiling analysis using ChIP-seq datasets (H3K27ac and H3K4me3) of liver from three ruminants.
