Project description:RNA-seq was used to measure gene expression in second instar larvae of the blow fly Lucilia sericata after feeding exposure (1 hour or 4 hours) to Pseudomonas aeruginosa and Acinetobacter baumanii. RNA-seq measurements were also collected from control larvae from each time point that were not exposed to bacteria.
2021-11-30 | GSE161305 | GEO
Project description:Seasonal influence of carrion blow fly (Diptera: Calliphoridae) colonization on the carcass microbiome
| PRJNA1427685 | ENA
Project description:Dolphins’ blow microbiome raw sequence reads
Project description:To understand the effects of the microbiome of Drosophila melanogaster on host gene expression, we compared the transcriptome of guts from conventionally reared flies to their axenically (germ-free)-reared counterparts. Our analysis used dissected intestines from 4-7 day-old adult females and included two wild-type fly lines, OregonR and CantonS, as well as an immune-deficient line, RelishE20. With one of the wild-type lines, CantonS, we also looked at the impact of microbiome on the transcriptional profile of dissected intestines from aged cohorts (35-40 day-old females) and young (4-7 day-old) non-gut tissues (all tissues remaining from samples dissected for the analysis of guts.
Project description:The primary objective of the present study was to use proteomic analysis as a non-targeted approach to initially identify and classify the proteome of respiratory exudate or blow in aquarium-based bottlenose dolphins.
Project description:We explore whether a low-energy diet intervention for Metabolic dysfunction-associated steatohepatitis (MASH) improves liver disease by means of modulating the gut microbiome. 16 individuals were given a low-energy diet (880 kcal, consisting of bars, soups, and shakes) for 12 weeks, followed by a stepped re-introduction to whole for an additional 12 weeks. Stool samples were obtained at 0, 12, and 24 weeks for microbiome analysis. Fecal microbiome were measured using 16S rRNA gene sequencing. Positive control (Zymo DNA standard D6305) and negative control (PBS extraction) were included in the sequencing. We found that low-energy diet improved MASH disease without lasting alterations to the gut microbiome.
