Project description:The nature of saprophytic and mycoparasitic hyphal growth of Trichoderma spp. has been studied extensively, yet information concerning conidial germination in this genus is lacking. Using near-synchronous germinating cultures of Trichoderma asperelloides, we followed the morphological progression from dormant conidia to initial polar growth, to germling formation and to evidence for first branching. We found that the stage-specific transcriptional profile of T. asperelloides is one of the most dynamic described to date; Transcript abundance of over 5000 genes, comprising approximately half of the annotated genome, was uniquely reduced in the transition from dormancy to polar growth, providing a potentially high degree of flexibility for germination on diverse substrates. Conversely, after the onset of germination, transcript abundance of approximately a quarter of the genome was exclusively elevated during transition from elongation to initial branching. Bayesian network analysis has identified several chitinases (including chi42) and glucanase-encoding genes as hallmarks of transcriptional hubs during germination. Furthermore, the fact that the expression of specific members of the chitin synthase and glucan elongase families was significantly increased during germination in the presence of Rhizoctonia solani, a known host of the mycoparasite, indicates that host recognition can occur during early stages of mycoprasite development.

2022-08-31 | GSE207066 | GEO

Trichoderma-plant root colonization: escaping early plant defense responses and activation of the antioxidant machinery

Project description:Trichoderma spp. are versatile opportunistic plant symbionts which can colonize the apoplast of plant roots. Microarrays analysis of Arabidopsis thaliana roots inoculated with Trichoderma asperelloides T203, coupled with qPCR analysis of 137 stress-responsive genes and transcription factors, revealed wide gene transcript reprogramming, preceded by a transient repression of the plant immune responses supposedly to allow root colonization. Enhancement in the expression of WRKY18 and 40, which stimulate JA-signaling via suppression of JAZ repressors and negative-regulate the expression of the defense genes FMO1, PAD3 and CYP71A13, was detected in Arabidopsis roots upon Trichoderma colonization. Reduced root colonization was observed in the wrky18/wrky40 double mutant line, while partial phenotypic complementation was achieved by over-expressing WRKY40 in the wrky18 wrky40 background. On the other hand, an increased colonization rate was found in roots of the FMO1 knockout mutant. Two-condition experiment: Roots treated with Trichoderma vs. Control untreated roots. Biological replicates: 2 control replicates, 2 treated replicates. 1 dye-swap.

2012-11-09 | E-GEOD-42113 | biostudies-arrayexpress

Trichoderma-plant root colonization: escaping early plant defense responses and activation of the antioxidant machinery

2012-11-09 | GSE42113 | GEO

Trichoderma reesei QM6a CGH self hybridization

Project description:We perform a self hybridisation comprative genomic hybridization (CGH) in order to validate the probe tiling design we done on Trichoderma reesei. This hybridization was done using QM6a wild type strain.

2010-03-16 | GSE17752 | GEO

Expression data from tomato (Solanum lycopersicum) plants in interaction with different Trichoderma strains

Project description:Trichoderma harzianum T34 is a fungal strain able to promote the plant growth and to increase plant defense responses. Trichoderma harzianum transformants expressing the amdS gene, encoding an acetamidase, of Aspergillus nidulans produce a higher plant development than the wild type T34. We used microarrays to analyze the physiological and biochemical changes in tomato plants produced as consequence of interaction with Trichoderma harzianum T34 and amdS transformants

2015-12-25 | GSE76332 | GEO

ArrayCGH of Trichoderma reesei strains QM9123, QM9414, NG14 and RutC-30

Project description:Trichoderma reesei is the main industrial producer of cellulases and hemicellulases used to depolymerize biomass in many biotechnical applications. Many production strains in use have been generated by classical mutagenesis. In this study we characterized genomic alterations in hyperproducing mutants of T. reesei by high-resolution comparative genomic hybridisation tiling array. We carried out aCGH analysis of four hyperproducing strains (QM9123, QM9414, NG14 and RutC-30) using QM6a genome as a reference. ArrayCGH analysis identified dozens of mutations in each strain analyzed.

2010-11-26 | GSE19606 | GEO

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