Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Affect of Aramchol on TGFβ stimulated H69 Cholangiocytes

ABSTRACT: Affect of Aramchol on TGFβ stimulated H69 Cholangiocytes

PROVIDER: PRJNA1293357 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Affect of Aramchol on TGFβ stimulated H69 Cholangiocytes

Project description:Cholestatic liver diseases, such as primary sclerosing cholangitis (PSC), are characterized by biliary fibroinflammation. Transforming growth factor-β (TGFβ) activated cholangiocytes release signals that recruit immune cells to drive inflammation and activate myofibroblasts to deposit the extracellular matrix (ECM). TGFβ regulates stearoyl-CoA desaturase (SCD) in stimulating lipid signaling. However, the role of SCD or its inhibitor, Aramchol, has not been investigated in biliary fibroinflammation. Here we used human transformed H69 cholangiocytes that were treated with TGFβ (10ng/mL) with and without Aramchol acid (30µM) overnight. RNA-seq analysis showed significant inhibition of TGFβ-induced hepatic fibrosis pathways while upregulating peroxisome proliferator-activated receptor (PPAR) signaling by Aramchol.

2025-07-23 | GSE303012 | GEO

Transcriptomic signature of treatment with recombinant laminin 511-E8 and acetazolamide in human H69 cholangiocytes

Project description:IgG4-related cholangitis (IRC) is the hepatobiliary manifestation of IgG4-related disease (IgG4-RD), a systemic, B-cell driven, fibroinflammatory disorder. To date, numerous autoantigens have been described including laminin 511-E8 and carbonic anhydrase (CA) enzymes (PMID 30089633, 15647194). Laminin 511-E8 is an extracellular matrix protein that has been shown to upregulate secretory components and aid in the cholangiocyte differentiation of induced pluripotent stem cells (PMID 27103433). Carbonic anhydrase enzymes catalyse the reversible hydration of carbon dioxide, thereby producing bicarbonate and a single proton. In humans there are 14 different CA isoforms with a confined subcellular distribution. All isoforms can be targeted by the pan-CA inhibitor acetazolamide, which is a registered drug for multiple indications. We aimed to investigate the role of IgG4-RD autoantigens laminin 511-E8 and CA enzymes in human cholangiocytes. Transcriptional profiling may provide insights into the underlying molecular mechanisms of these proteins on the cholangiocellular level. Thus, by RNA sequencing the transcriptomic signature of recombinant laminin 511-E8 and acetazolamide treatment was assessed in human H69 cholangiocytes.

2024-02-15 | GSE221746 | GEO

MicroRNA profiling of human cholangiocyte H69 cells after exposure to tumor necrosis factor-alpha

Project description:H69 cells (human cholangiocytes) were exposed to TNF-alpha (10 ng/ml) for 8 h and were then collected for miRNA analysis, compared with non-treated control cells.

2011-01-01 | E-MEXP-2049 | biostudies-arrayexpress

Transcriptomic signature of treatment with recombinant laminin 511-E8 and acetazolamide in human H69 cholangiocytes

Project description:Transcriptomic signature of treatment with recombinant laminin 511-E8 and acetazolamide in human H69 cholangiocytes

| PRJNA915951 | ENA

Gene expression after TSA treatment in H69 cells

Project description:Objective of the study is to determine global changes in gene expression after HDAC inhibitor Trichostatin (TSA) treatment in H69 cell line

2021-05-26 | GSE161313 | GEO

MicroRNA profiling of human cholangiocyte H69 cells after culturing with lipopolysaccharide

Project description:H69 cells were cultured in H69 medium with 1 ng/ml lipopolysaccharide(LPS, for smaples 04, 05 and 06) or without LPS(for samples 01, 02 and 03) for 8 hours and then collected for array analysis. <br>

2010-02-11 | E-MEXP-2052 | biostudies-arrayexpress

Gene expression from H69M versus H69 SCLC cell lines

Project description:H69M cells derive from H69 small cell lung cancer cells subjected to prolonged treatment with HGF. Among the whole population of cells, a subset of more fibroblastic cells was isolated (H69M-mesenchymal). In this experiment we compared expression profiles of both cell lines Keywords: Expression Profiling by array We analyzed 3 arrays from each condition:H69 versus H69M SCLC cell lines

2013-12-31 | E-GEOD-45120 | biostudies-arrayexpress

Staphylococcus hominis strain:H69

Project description:Staphylococcus hominis strain:H69 Genome sequencing and assembly

| PRJNA316465 | ENA

cholangiocytes treated with TGFbeta

Project description:cholangiocytes treated with TGFbeta

| PRJNA606515 | ENA

Gene expression from H69M versus H69 SCLC cell lines

2013-12-31 | GSE45120 | GEO