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The Dream and MEC Complexes reinforce SPR-5 MET-2 maternal reprogramming to maintain the Germline-Soma distinction [lin-35 RNAseq]

ABSTRACT: The Dream and MEC Complexes reinforce SPR-5 MET-2 maternal reprogramming to maintain the Germline-Soma distinction [lin-35 RNAseq]

PROVIDER: PRJNA1293434 | ENA |

REPOSITORIES: ENA

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The Dream and MEC Complexes reinforce SPR-5; MET-2 maternal reprogramming to maintain the Germline-Soma distinction [lin-35 RNAseq]

Project description:Proper regulation of tissue-specific gene expression is essential for cell fate determination and development. In Caenorhabditis elegans, the distinction between germline and somatic cell fates is partially established through maternally inherited histone modifications. During gametogenesis, germline genes acquire transcription-coupled chromatin marks such as histone methylation, which are inherited by the zygote and must be reprogrammed at fertilization to prevent inappropriate germline gene expression in somatic tissues. This maternal reprogramming is mediated by histone-modifying enzymes, including the demethylase SPR-5/LSD1 and the H3K9 methyltransferase MET-2/SETDB1. Together, these enzymes limit DNA accessibility to transcriptional machinery, thereby resetting the chromatin landscape in te newly formed zygote. Failure to reprogram inherited histone methylation, as in spr-5; met-2 mutants, results in the ectopic expression of MES-4-targeted germline genes in somatic tissues, leading to developmental delay and sterility. Here, we investigated whether transcriptional repressor complexes—the DREAM and MEC NuRD complexes—cooperate with SPR-5 and MET-2 to specify germline versus soma. Loss of DREAM or MEC NuRD components alone causes somatic expression of germline genes. Strikingly, combined loss with spr-5; met-2 enhances developmental arrest at the L1 stage and exacerbates germline gene misexpression. By re analyzing previous published ChIP-seq data, we confirmed direct binding of MEP-1 and LIN-35 to these misregulated genes. Our findings demonstrate that maternal reprogramming and conserved chromatin repressor complexes act synergistically to repress germline transcription in somatic tissues, ensuring proper somatic development and preventing soma-to-germline transformation.

2025-07-19 | GSE303062 | GEO

The Dream and MEC Complexes reinforce SPR-5; MET-2 maternal reprogramming to maintain the Germline-Soma distinction [mep-1 RNAseq]

2025-07-21 | GSE303085 | GEO

The Dream and MEC Complexes reinforce SPR-5 MET-2 maternal reprogramming to maintain the Germline-Soma distinction [mep-1 RNAseq]

Project description:The Dream and MEC Complexes reinforce SPR-5 MET-2 maternal reprogramming to maintain the Germline-Soma distinction [mep-1 RNAseq]

| PRJNA1294132 | ENA

The DREAM complex functions as conserved master regulator of somatic DNA repair capacities

Project description:Mutations in the DREAM complex induce germline-like gene expression patterns including the increase of multiple mechanisms of DNA repair in the soma of Caenorhabditis elegans. This confers mutants of the DREAM complex resistance to a wide range of DNA damage types during development and aging.

2023-01-31 | PXD033836 | Pride

DREAM Interrupted: Severing LIN-35-MuvB association in Caenorhabditis elegans impairs DREAM function but not its chromatin localization

Project description:The highly conserved DREAM transcriptional repressor complex contains an RB-like pocket protein, an E2F-DP transcription factor heterodimer, and the 5-subunit MuvB complex. Using CRISPR/Cas9 targeted mutagenesis, we disrupted the interaction between the sole Caenorhabditis elegans pocket protein LIN-35 and the MuvB subunit LIN-52. A triple alanine substitution of LIN-52's LxCxE motif (3A) severed LIN-35-MuvB association and caused classical DREAM mutant phenotypes, including synthetic multiple vulvae, high-temperature arrest, and ectopic expression of germline genes in the soma. We performed RNA-seq in lin-52(3A) mutant late embryos (4 replicates) compared to lin-52(WT) wild-type late embryos (4 replicates) to assess the genome-wide effects on gene expression that result from severing LIN-35-MuvB association.

2022-05-24 | GSE199287 | GEO

MEC-sludge

Project description:MEC-sludge Genome sequencing

| PRJNA482498 | ENA

MEC Bioreactor

Project description:MEC Bioreactor Raw sequence reads

| PRJNA715352 | ENA

Loss of the Caenorhabditis elegans pocket protein LIN-35 reveals MuvB’s innate function as the repressor of DREAM target genes

Project description:The Retinoblastoma-like pocket proteins p130 and p107 act as gatekeepers of the cell cycle through their activity within the DREAM (Dp/Rb-like/E2F/MuvB) transcriptional repressor complex. The goal of this study was to address how the pocket protein contributes to DREAM complex assembly and function on chromatin by utilizing a protein null mutant of the only C. elegans pocket protein LIN-35. We performed ChIP-seq of C. elegans DRM subunits in wild-type and lin-35 null late embryos to assess the effect on their chromatin localization following loss of LIN-35.

2017-10-25 | GSE95071 | GEO

DREAM On, DREAM Off: A Review of the Estrogen Paradox

Project description:DREAM On, DREAM Off: A Review of the Estrogen Paradox

| PRJNA1114009 | ENA

DREAM Interrupted: Severing LIN-35-MuvB association in Caenorhabditis elegans impairs DREAM function but not its chromatin localization

Project description:DREAM Interrupted: Severing LIN-35-MuvB association in Caenorhabditis elegans impairs DREAM function but not its chromatin localization

| PRJNA819162 | ENA

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