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Mus musculus

ABSTRACT: Analysis of mir-223 knockout cultured neutrophils versus wild-type cultured neutrophils, by ribosome profiling and mRNA-Seq

PROVIDER: PRJNA129411 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR065774.fastq.gz	Fastqsanger.gz
	SRR065775.fastq.gz	Fastqsanger.gz
	SRR065776.fastq.gz	Fastqsanger.gz
	SRR065777.fastq.gz	Fastqsanger.gz
	SRR065778.fastq.gz	Fastqsanger.gz

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Mammalian microRNAs predominantly act to decrease target mRNA levels

Project description:This SuperSeries is composed of the following subset Series: GSE21992: Analysis of HeLa cells after transfection with miR-1 or miR-155, by ribosome profiling and mRNA-Seq GSE22001: Analysis of mir-223 knockout cultured neutrophils versus wild-type cultured neutrophils, by ribosome profiling and mRNA-Seq GSE22002: Analysis of HeLa cells after transfection with miR-1 or miR-155, by microarray profiling GSE22003: Analysis of mir-223 knockout cultured neutrophils versus wild-type cultured neutrophils, by microarray profiling Refer to individual Series

2010-08-02 | E-GEOD-22004 | biostudies-arrayexpress

Analysis of mir-223 knockout cultured neutrophils versus wild-type cultured neutrophils, by ribosome profiling and mRNA-Seq

Project description:MicroRNAs (miRNAs) are endogenous ~22-nucleotide RNAs that mediate important gene-regulatory events by pairing to the mRNAs of protein-coding genes to direct their repression. Repression of these regulatory targets leads to decreased translational efficiency and/or decreased mRNA levels, but the relative contributions of these two outcomes have been largely unknown, particularly for endogenous targets expressed at low-to-moderate levels. Here, we use ribosome profiling to measure the overall effects on protein production and compare these to simultaneously measured effects on mRNA levels. For both ectopic and endogenous miRNA regulatory interactions, lowered mRNA levels account for most (≥84%) of the decreased protein production. These results show that changes in mRNA levels closely reflect the impact of miRNAs on gene expression and indicate that destabilization of target mRNAs is the predominant reason for reduced protein output. Examine ribosome footprints and mRNA abundance of mir-223 knockout cultured neutrophils, versus wild-type cultured neutrophils Supplementary processed data file linked below. mir223_summaryTable.txt: log2 fold changes (miR-223-knockout versus wild-type neutrophils).

2010-08-03 | E-GEOD-22001 | biostudies-arrayexpress

Mus musculus

Project description:Wild-type cultured neutrophils versus miR-223 null cultured neutrophils

| PRJNA113835 | ENA

Mus musculus

Project description:Analysis of mir-223 knockout cultured neutrophils versus wild-type cultured neutrophils, by microarray profiling

| PRJNA129167 | ENA

Analysis of mir-223 knockout cultured neutrophils versus wild-type cultured neutrophils, by microarray profiling

Project description:MicroRNAs (miRNAs) are endogenous ~22-nucleotide RNAs that mediate important gene-regulatory events by pairing to the mRNAs of protein-coding genes to direct their repression. Repression of these regulatory targets leads to decreased translational efficiency and/or decreased mRNA levels, but the relative contributions of these two outcomes have been largely unknown, particularly for endogenous targets expressed at low-to-moderate levels. Here, we use ribosome profiling to measure the overall effects on protein production and compare these to simultaneously measured effects on mRNA levels. For both ectopic and endogenous miRNA regulatory interactions, lowered mRNA levels account for most (≥84%) of the decreased protein production. These results show that changes in mRNA levels closely reflect the impact of miRNAs on gene expression and indicate that destabilization of target mRNAs is the predominant reason for reduced protein output. Examine mRNA expression levels in mir-223 knockout cultured neutrophils, versus wild-type cultured neutrophils.

2010-08-03 | E-GEOD-22003 | biostudies-arrayexpress

Mus musculus

Project description:Wild-type neutrophils and miR-223 null neutrophils

| PRJNA114295 | ENA

Wild-type cultured neutrophils versus miR-223 null cultured neutrophils

Project description:This array analysis is to study the regulation of target messages’ expression in in vitro cultured murine neutrophils versus miR-223 null neutrophils. Culture media was SILAC-IMDM for MS analysis.

2008-07-31 | GSE11973 | GEO

Wild-type neutrophils and miR-223 null neutrophils

Project description:This array analysis is to study the regulation of target messages’ expression in murine neutrophils versus miR-223 null neutrophils.

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4 days cultured progenitors and 8 days cultured mature neutrophils from WT vs miR-223 null neutrophils

Project description:This array analysis is to study developmental time course of the regulation of target messages’ expression during culture of murine neutrophils versus miR-223 null neutrophils. Culture media was SILAC-IMDM for MS analysis.

2008-07-30 | GSE12003 | GEO

Analysis of small RNAs in murine neutrophils by Solexa/Illumina genome analyzer

Project description:To examine the effects of microRNAs including miR-223 on murine neutrophil function, small RNAs cloning and sequencing from neutrophils were performed. Neutrophils were isolated from a wild-type mouse and total RNAs were prepared. Small RNAs were cloned and sequenced by Solexa/Illumina genome analyzer.

2008-07-31 | E-GEOD-12074 | biostudies-arrayexpress

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