Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1294429 | ENA |
REPOSITORIES: ENA
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Project description:The widespread presence of microplastics (MPs) in freshwater systems has raised concerns about their potential ecotoxicity on aquatic organisms. In this study, we evaluated the effects of four MPs with different compositions, namely polyethylene (PE), polystyrene (PS), polyvinyl chloride (PVC) and polypropylene (PP), on freshwater microalgae Chlamydomonas reinhardtii. PS and PVC MPs caused greater growth inhibition and stronger oxidative stress response than PP and PE MPs. Proteomics analysis was employed to explore the mechanical understanding of the composition-dependent toxicity of MPs on microalgae. Proteins involved in photosynthesis processes were identified as contributors to the diverse responses of microalgae to differently composed MPs. Photosynthesis activity of algae, including pigment content and photoprotective response, was determined to reflect the distinct effect of the four MPs. The indicated down-regulated expression of photosynthetic proteins by proteomics analysis was further confirmed using western blot, with PVC and PS showing greater impacts on their expression reduction. Our findings not only show the composition-dependent effect of MPs on microalgae but also provide important insights into the molecular mechanism of MPs’ toxicity on natural phytoplankton species.
| PXD052887 |
Project description:we studied the effect that 16.4 µm fragment type polypropylene (PP) MPs, which have an irregular shape and sharp edges and form naturally in the environment, had on breast cancer PPMP incubation for 24 hours in the MDA-MB-231 cells significantly altered the level of cell cycle-related transcripts in an RNA-seq analysis
2023-04-26 | GSE219207 | GEO
Project description:Microplastics are defined as plastics ranging in size from 0.1μm to 5mm. Currently, research is being conducted across various fields to examine the effects of microplastics. Some studies demonstrated negative impacts on cells and mice. However, there is a lack of research on the effects by long-term exposure to microplastics. Most of the papers evaluated cytotoxicity with period of less than 2 months. Therefore, in this study, we investigated the potential issues that may arise from prolonged exposure through food mixed with Polypropylene microplastic (PP-MP) for over a year. We divided our study into short, mid, and long-term periods to assess cytotoxicity through Glucose tolerance test, Insulin tolerance test, analysis of insulin and c-peptide levels, hanging, grip, treadmill, Y-maze and open field tests, Respiratory Exchange Ratio, Energy Expenditure, Activity, and body composition. Through this, we comprehensively examined potential issues related to mouse behavior, muscle, metabolism and other factors. After dissection, RNA sequencing was carried out to investigate the effects on genes. For further verification, RT-qPCR was conducted. To summarize, our study provides evidence suggesting that treatment of microplastics for a short term has adverse effects, but with prolonged exposure, their effects tend to diminish.
2025-04-17 | GSE286116 | GEO
Project description:Anaerobic ammonium oxidation (anammox) emerges as a sustainable solution for nitrogen removal in sewage, but it is susceptible to stress induced by xenobiotics that are ubiquitous in sewage. Despite wide recognition of this critical issue, a comprehensive understanding of the molecular and ecological mechanisms underlying the response of anammox consortia to xenobiotic stress remain elusive. Here, we integrated multi-omics approaches with biofilm reactor operation to unravel how bisphenol A (BPA, a representative xenobiotic) perturbs anammox consortia across environmentally relevant concentrations. We show that anammox consortia tolerated low BPA levels (0.2–2 mg/L), where nitrogen removal efficiency transiently declined and subsequently recovered, aided by a 30.9% increase in quorum-sensing (QS) signal C6-HSL. By contrast, exposure to ≥10 mg/L BPA caused severe and irreversible inhibition, with total inorganic nitrogen removal dropping to 17.8%. High BPA concentrations suppressed QS signaling, intensified oxidative stress, and compromised membrane integrity, leading to enzymatic inhibition and transcriptional repression of anammox functional genes. Multi-omics evidence revealed that BPA stress also promoted horizontal transfer of the BPA-degrading gene bisdA via extracellular DNA, suggesting a new community-level adaptive mechanism. Metagenomic and metabolomic analyses further indicated BPA-driven restructuring of microbial networks, namely high BPA levels favored denitrifiers and BPA degraders while suppressing anammox bacteria, and triggered metabolic reprogramming toward xenobiotic degradation at the expense of nucleotide and amino acid biosynthesis. Together, these findings reveal a multifaceted collapse mechanism of anammox under BPA stress, providing a mechanistic basis for designing strategies to safeguard microbial nitrogen removal in xenobiotic-laden wastewaters.
2026-03-27 | MTBLS14153 | MetaboLights
Project description:4D label-free metaproteomic technique was used to reveal the protein expressions of anammox consortia under different flow shear stress (represented by different stirring speeds: 40, 120, and 200 rpm).
| PXD079362 |
Project description:Microplastics (MPs) as widespread contamination pose high risk for aquatic organisms.Intestinal microbiotahas have high interaction with immune system of host body. In this study, intestinal microbiota of zebrafish after Polystyrene (PS-MPs) exposure were characterized by 16S rDNA amplicon sequencing. We found that 100nm and 200μm PS-MPs exposure significantly increased diversity of intestinal microbiota and all the three sizes of PS-MPs increased abundance of pathogenic bacteria.
2019-10-01 | GSE136108 | GEO
Project description:Plastic is widespread in our lives, releasing various Microplastics (MPs) with toxicity. In recent years, the potential threat of MPs on reproductive system arouses public concern. Numerous reports have focused on its damage to spermatogenesis. Nevertheless, the toxicity of MPs on female reproduction is unclear. Here, we explored this question using bovine oocyte. We found that MPs disrupts spindle organization, chromosomes alignment and actin assembly, leading to failed maturation of bovine oocytes. Meanwhile, cortical granule secretion is obstructed, suggesting the failure of cytoplasmic maturation. Therefore, embryonic development is impacted. In transcriptome results, MPs induces the expression change of Mitochondrial-related genes, reflecting the damage of MPs are mediated by mitochondrial functions. MPs indeed causes oxidative stress, DNA damage and apoptosis. Taurine is capable for stabilizing cellular antioxidant level. We surprisingly find that Taurine suppresses mitochondrial dysfunction, reversing the failure of oocyte maturation and embryo development after MPs exposure. Collectively, we reveal the reproduction toxicity of MPs on bovine oocytes, and demonstrates the restorative effect of Taurine against MPs.
2025-08-07 | GSE295716 | GEO
Project description:Microplastics are emerging environmental pollutants with potential biological effects on human health. However, the molecular responses of colorectal cancer tissues following microplastic exposure remain poorly characterized. In this study, we performed bulk RNA sequencing to investigate transcriptomic alterations associated with polystyrene microplastics (PS-MPs) exposure in a murine colorectal cancer model. CT26 colorectal cancer cells were subcutaneously implanted into BALB/c mice to establish xenograft tumors. After tumor formation, mice received daily intratumoral injections of 1 μm PS-MPs suspension (10 μg/mL, 50 μL/day), while control mice received no PS-MPs treatment. Tumor tissues were collected for RNA extraction and high-throughput sequencing. Differential gene expression analysis and downstream bioinformatic analyses were performed to characterize molecular changes associated with PS-MPs exposure. This dataset provides transcriptomic profiles of CT26 colorectal cancer xenograft tumors following PS-MPs exposure and may facilitate further investigation of the biological responses associated with environmental microplastic exposure.
2026-08-23 | GSE343346 | GEO
Project description:Microplastics (MPs) as widespread contamination pose high risk for aquatic organisms. However, current understanding of MP toxicities are based on cell population-averaged measurements. Here we used single-cell RNA sequencing to provide the transcriptome heterogeneity of 12000 intestinal cells obtained from zebrafishes exposed to 100nm, 5μm and 200μm polystyrene MPs (PS-MPs) for 21 days. Eight intestinal cell populations were identified. We found that all the three sizes of PS-MPs induced dysfunction of intestinal immune cells (including phagosome and regulation of immune system process).
2019-10-01 | GSE135767 | GEO
Project description:Environmental aging processes, such as oxidation, can significantly alter the physicochemical properties and toxicity profiles of microplastics (MPs). Here, we investigated the toxicological impact of pristine polyethylene (PE) and oxidized polyethylene (OPE) microplastics using a dual-species aquatic model comprising Daphnia magna and zebrafish embryos. Characterization confirmed OPE particles exhibited increased surface roughness, higher surface charge, and broader size distribution compared to PE. Exposure to OPE induced pronounced lipid accumulation and reduced heart rate in both models. Transcriptomic analysis revealed that OPE suppressed key lipid transport and metabolism genes, including mttp, apoea, and apobb. These effects were validated through qPCR and lipid staining techniques. Notably, zebrafish embryos exposed to OPE showed significant developmental impairment even with intact chorions, suggesting enhanced bioavailability of oxidized particles. This study highlights the amplified toxicity of aged microplastics and underscores the importance of evaluating environmentally relevant forms when assessing the ecological risk of MPs.
2026-07-01 | GSE304012 | GEO