Profound effect of asparagine-linked glycosylation protein 11 (TgALG11) on genomic expression of Toxoplasma gondii
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ABSTRACT:
PROVIDER: PRJNA1294725 | ENA |
REPOSITORIES: ENA
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Project description:Toxoplasma gondii is an intracellular parasite that relies on complex molecular mechanisms to support its proliferation and survival. In this study, we investigate the role of TgALG11 in these processes and explore the underlying regulatory network. Our data demonstrate that TgALG11 is essential for maintaining T. gondii proliferation and for normal N-glycosylation. To further elucidate the molecular mechanisms underlying the essential functions of TgALG11, we performed transcriptome sequencing to identify the genes regulated by this factor. Total RNA was extracted from iTgALG11 strains cultured with 0.5 μg/ml ATc (knockdown strain) and DMSO (control), and subjected to deep sequencing using the Illumina Novaseq™ 6000 platform. The sequence reads were subsequently mapped to the T. gondii genome, resulting in a comprehensive transcriptome encompassing 8,948 genes. Among these, 934 genes were differentially expressed due to TgALG11 deficiency, including 315 downregulated and 5 upregulated genes (DESeq, |log2(FoldChange)| ≥ 1, padj ≤ 0.05). Functional enrichment analysis revealed that these genes were associated with various Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Our findings provide novel insights into the profound impact of TgALG11 on the T. gondii transcriptome, underscoring its critical role in parasite biology.
2025-07-27 | GSE303275 | GEO
Project description:Toxoplasma gondii is an intracellular parasite that relies on complex molecular mechanisms to support its proliferation and survival. In this study, we investigate the role of TgALG11 in these processes and explore the underlying regulatory network. Our data demonstrate that TgALG11 is essential for maintaining T. gondii proliferation and for normal N-glycosylation. To further elucidate the molecular mechanisms underlying the essential functions of TgALG11, we performed transcriptome sequencing to identify the genes regulated by this factor. Total RNA was extracted from iTgALG11 strains cultured with 0.5 μg/ml ATc (knockdown strain) and DMSO (control), and subjected to deep sequencing using the Illumina Novaseq™ 6000 platform. The sequence reads were subsequently mapped to the T. gondii genome, resulting in a comprehensive transcriptome encompassing 8,948 genes. Among these, 8549 genes were differentially expressed due to TgALG11 deficiency, including 1307 downregulated and 1951 upregulated genes (DESeq, |log2(FoldChange)| ≥ 1, q ≤ 0.05). Functional enrichment analysis revealed that these genes were associated with various Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Our findings provide novel insights into the profound impact of TgALG11 on the T. gondii transcriptome, underscoring its critical role in parasite biology.
2025-12-28 | GSE314937 | GEO
Project description:Profound effect of asparagine-linked glycosylation protein 11 (TgALG11) on genomic expression of Toxoplasma gondii II
| PRJNA1393156 | ENA
Project description:Toxoplasma gondii is an intracellular parasite that relies on intricate molecular mechanisms for its proliferation and survival. Here, we investigate the role of TgUAE1 in these processes and shed light on the underlying regulatory network. We demonstrate that TgUAE1 is crucial for maintaining the proliferation of T. gondii and the normal morphology of mitochondria. To elucidate the molecular basis of TgUAE1's essential functions, we conducted transcriptome sequencing to identify the genes regulated by this factor. Total RNA was extracted from TATi-3HA-TgUba1 strains cultured under 0.5 μg/ml ATc (knockout strain) and DMSO (wild strain), and subjected to deep sequencing using the Illumina Novaseq TM 6000 platform. Subsequently, sequence reads were mapped to the T. gondii genome, resulting in a comprehensive transcriptome containing 8637 detected genes. Among these genes, 934 were dysregulated in the absence of TgUAE1, comprising 60 downregulated and 874 upregulated genes. Functional enrichment analysis revealed their involvement in various GO categories and KEGG pathways. Our findings provide novel insights into the profound impact of TgUAE1 on the expression landscape of the T. gondii genome, highlighting the significance of this factor in parasite biology.
2023-08-16 | GSE240648 | GEO
Project description:Recent advances in high throughput sequencing methodologies allow the opportunity to probe in depth the transcriptomes of organisms including N. caninum and Toxoplasma gondii. In this project, we are using Illumina sequencing technology to analyze the transcriptome (RNA-Seq) of experimentally accessible stages (e.g. tachyzoites at different times points) of T. gondii VEG strain. The aim is to make comparative transcriptional landscape maps of Neospora and Toxoplasma at different time points at different life cycle stages and compare levels of expression of orthologous genes in these two organisms.
2011-02-08 | E-MTAB-550 | biostudies-arrayexpress
Project description:This SuperSeries is composed of the following subset Series: GSE11437: Expression QTL mapping of Toxoplasma gondii genes, Bradyzoite array GSE11514: Expression QTL mapping of Toxoplasma gondii genes, Tachyzoite array Keywords: SuperSeries Refer to individual Series
2008-05-22 | E-GEOD-11515 | biostudies-arrayexpress