Project description:Alfalfa seeds from Gwadar Port, Pakistan were cultivated at Central South University of Forestry and Technology under controlled conditions (28 °C, 70% RH, 12 h light-dark cycle) for two months.
Project description:Increasing evidence indicates that despite exposure to harsh environmental stresses, Salmonella enterica successfully persists on plants, utilizing fresh produce as a vector to animal hosts. This study examined the mechanisms of S. enterica plant colonization, including the role of the diguanylate cyclase AdrA. Utilizing microarray analysis, we identified a new function for AdrA in the transcriptional regulation of colanic acid biosynthesis genes. Changes in gene expression, comparing wildtype to an adrA mutant, were measured in planktonic bacterial cells after 24-hour incubation with alfalfa seeds. Two independent experiments were performed for each strain (wildtype and the adrA mutant).
Project description:Two potato cultivars, Russet Burbank and Bionta, were inoculated with three different endophytes containing different AHL types. The impact of the endophytes to the different cultivars was measured by gene expression analysis with a customized microarray
Project description:We seek to determine the effects of foliar endophytes on soybean physiological traits, and their effect on plant responses to elevated carbon dioxide. We are using three dominant endophytes extracted from the SoyFACE facility at UIUC.