Project description:Alfalfa seeds from Gwadar Port, Pakistan were cultivated at Central South University of Forestry and Technology under controlled conditions (28 °C, 70% RH, 12 h light-dark cycle) for two months.
Project description:Increasing evidence indicates that despite exposure to harsh environmental stresses, Salmonella enterica successfully persists on plants, utilizing fresh produce as a vector to animal hosts. This study examined the mechanisms of S. enterica plant colonization, including the role of the diguanylate cyclase AdrA. Utilizing microarray analysis, we identified a new function for AdrA in the transcriptional regulation of colanic acid biosynthesis genes. Changes in gene expression, comparing wildtype to an adrA mutant, were measured in planktonic bacterial cells after 24-hour incubation with alfalfa seeds. Two independent experiments were performed for each strain (wildtype and the adrA mutant).
Project description:Two alfalfa varieties contrasting in heat tolerance, MS30 and MS37, were used for this experiment, and their seeds were provided by Sichuan Academy of Grassland Sciences. After two months of growth, both alfalfa varieties grow into adult-plants. Select 10-15 healthy plants with consistent growth to remain in each nutritional bowl. All materials are transferred to artificial climate boxes for high temperature stress treatment. The light cycle is still the day/night cycle:16/8h. According to the results of a prior experiment and based on the relevant report, the treatment temperature was set to 20℃, 25℃, 30℃, 35℃, 40℃ and 4℃, which is a gradient upward trend. Among them, alfalfa growing at 20°C were used as the control (non-HT stress). All alfalfa varieties were treated at each temperature gradient for 7 days and the leaves was collected for the determination of physiological indicators. Moreover, the physiological conditions of the plants and the humidity of the climate box were observed at any time. water the plants properly once a day, and the alfalfa special fertilizer was applied once in each cycle of temperature stress treatment to ensure the water and nutrition demand of the plant. Based on the results of the above experiment, some physiological responses demonstrated that most alfalfa varieties showed the most significant variation at 20℃, 35℃ and 43℃. Therefore, leaf samples collected from plants after exposure to the rising temperature 7 days were harvested for further TMT quantitative proteomic analysis.
Project description:We seek to determine the effects of foliar endophytes on soybean physiological traits, and their effect on plant responses to elevated carbon dioxide. We are using three dominant endophytes extracted from the SoyFACE facility at UIUC.
