Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1294804 | ENA |
REPOSITORIES: ENA
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Project description:Seeds, as an effective depository of plant genetic resources and an important raw material for germplasm innovation, aging or deterioration will directly lead to a reduction in germination rate, vigor and viability, inhibit the normal development of the seed embryo and seedling growth. Methods: Allium mongolicum seeds stored naturally for 2, 4 and 5 years were used as test materials, determination of germination indexes, antioxidant enzyme activities and other physiological indexes in seeds treated with 0.8 mmol/L spermidine solution, and proteomic and transcriptomic sequencing were performed. Results: The results showed that the germination ability of Allium mongolicum seeds decreased continuously with the extension of storage life, and the germination rate was only about 40% after 5 years of storage. The relative conductivity, MDA and H2O2 content were significantly higher, CAT, POD, SOD, APX, and respiratory rate were significantly reduced; Spd treatment increased seed germination rate, significantly reduced MDA, H2O2 content and relative conductivity, and increased CAT, POD, SOD, and APX activities. Functional analysis of the differentially expressed proteins showed that the differentially expressed proteins were mainly concentrated in energy metabolism pathways such as carbon metabolism, amino acid biosynthesis, glycolysis/glycogen isomerization, TCA cycle, pyruvate metabolism and pentose phosphate metabolism. Transcriptome sequencing yielded similar results. PPI analysis identified 10 proteins related to the aging of Allium mongolicum seeds, and the core protein was also verified by qPCR, the gene expression trend was basically consistent with the proteomic assay results. Conclusions : Energy metabolism may be a key pathway in the aging of Allium mongolicum seeds, and spermidine can effectively alleviate Allium mongolicum seed aging. The results enriched the molecular mechanism of seed storability of Allium mongolicum and provided theoretical basis for molecular marker-assisted breeding of storability traits in Allium mongolicum L.
2024-11-18 | PXD058020 |
Project description:The purpose of this study was to use chemical similarity evaluations, transcriptional profiling, in vitro toxicokinetic data and physiologically based pharmacokinetic (PBPK) models to support read across for a series of branched carboxylic acids using valproic acid (VPA), a known developmental toxicant, as a comparator. The chemicals included 2-propylpentanoic acid (VPA), 2-ethylbutanoic acid (EBA), 2-ethylhexanoic acid (EHA), 2-methylnonanoic acid (MNA), 2-hexyldecanoic acid (HDA), 2-propylnonanoic acid (PNA), dipentyl acetic acid (DPA) or 2-pentylheptanoic acid (PHA), octanoic acid (OA, a straight chain alkyl acid) and 2-ethylhexanol. Transcriptomics was evaluated in four cell types (A549, HepG2, MCF7 and iCell cardiomyocytes) 6 hours after exposure to 3 concentrations of the compounds, using the L1000 platform. The transcriptional profiling data indicate that two- or three-carbon alkyl substituents at the alpha position of the carboxylic acid (EHA and PNA) elicit a transcriptional profile similar to the one elicited by VPA. The transcriptional profile is different for the other chemicals tested, which provides support for limiting read across from VPA to much shorter and longer acids. Molecular docking models for histone deacetylases, the putative target of VPA, provides a possible mechanistic explanation for the activity cliff elucidated by transcriptomics. In vitro toxicokinetic data was utilized in a PBPK model to estimate internal dosimetry. The PBPK modeling data show that as the branched chain increases, predicted plasma Cmax decreases. This work demonstrates how transcriptomics and other mode of action-based methods can improve read across.
2024-11-01 | GSE218438 | GEO
Project description:Transcriptome Analysis of Allium mongolicum Regel: Drought Resistance Comparison Between Wild (Minqin, GS) and Domesticated (Tongliao, XH) Accessions
| PRJNA1277023 | ENA