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MERFISH profiling of mouse retinal ganglion cell layer on wholemount

ABSTRACT: MERFISH profiling of mouse retinal ganglion cell layer on wholemount

PROVIDER: PRJNA1295347 | ENA |

REPOSITORIES: ENA

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MERFISH profiling of mouse retinal ganglion cell layer on wholemount

Project description:We optimized a workflow combining imaging-based spatial transcriptomics (MERFISH) and immunostaining on ganglion cell layer retinal flatmounts of C57/Bl6J mice.The MERFISH data shows molecularly-defined retinal ganglion cell types types exhibited non-uniform distributions. We also analyzed local neighborhoods for each cell and registered several RGC types as enriched in the perivascular niche.

2025-07-23 | GSE303399 | GEO

Transcription profiling of mouse developing retinal ganglion and amacrine cells reveals molecular heterogeneity of

Project description:During development of the central nervous system (CNS), cycling uncommitted progenitor cells give rise to a variety of distinct neuronal and glial cell types. As these different cell types are born, they progress from newly specified cells to fully differentiated neurons and glia. In order to define the developmental processes of individual cell types, single cell expression profiling was carried out on developing ganglion and amacrine cells of the murine retina. Individual cells from multiple developmental stages were isolated and profiled on Affymetrix oligonucleotide arrays. These experiments have yielded an expanded view of the processes underway in developing retinal ganglion and amacrine cells, as well as several hundred new marker genes for these cell types. In addition, this study has allowed for the definition of some of the molecular heterogeneity both between developing ganglion and amacrine cells and among subclasses of each cell type. Experiment Overall Design: Single retinal cells were isolated in tubes containing lysis buffer, their mRNAs were reverse transcribed, and the resulting cDNAs were PCR amplified for 35 cycles. Labeled cDNA samples were hybridized to Affymetrix 430 2.0 microarrays and the data was normalized using MAS5.0 software. Cells were identified post hoc as either developing retinal ganglion cells, amacrine cells or rod photoreceptor cells.

2008-06-16 | E-GEOD-9812 | biostudies-arrayexpress

Subcellular expression profiling of the growth cones of retinal ganglion cells (RGC)

Project description:Cue-directed axon guidance depends partly on local translation in growth cones. Many mRNA transcripts are known to reside in developing axons yet little is known about their subcellular distribution or, specifically, which transcripts are in growth cones. Laser capture microdissection (LCM) was used to isolate the growth cones of retinal ganglion cell (RGC) axons of two vertebrate species, mouse and Xenopus, coupled with unbiased genome-wide microarray profiling. Localized mRNA from the isolated growth cones of Xenopus laevis and Mus musculus retinal ganglion cells were subjected to microarray analysis

2010-11-23 | E-GEOD-25166 | biostudies-arrayexpress

Laser capture microdissection of the ganglion cell layer for analyzing transcriptional responses induced by demyelination

Project description:Demyelination and loss of oligodendrocytes deprives neurons of crucial metabolic and trophic support and contributes to neurodegeneration. However, the precise mechanisms that trigger neurodegeneration during demyelination remain unclear. Here, we examine transcriptional differences in neurons that have been demyelinated relative to myelinated neurons. Demyelination is induced by the knockout of Myrf, a transcription factor essential for myelin gene expression and oligodendrocyte identity, from oligodendrocyte lineage cells using Myrffl/fl Sox10 CreERT mice. We performed laser capture microdissection of the ganglion cell layer, which contains the majority of the retinal ganglion cells (RGC), a nearly ubiquitously myelinated population of neurons. We find that dissection of the ganglion cell layer greatly enriches for RGC transcripts relative to controls. Demyelinated Myrffl/fl Sox10 CreERT mice have upregulation of Hrk and Ecel1 in the ganglion cell layer, which are known to be upregulated during axonal injury and driven by dual leucine zipper kinase (DLK) signaling. These data suggest that demyelination may activate a DLK-mediated signaling cascade that culminates in transcriptional changes that are often associated with apoptosis of neurons in the central nervous system.

2024-09-11 | GSE245362 | GEO

RNA-Seq and ChIP-Seq gene profiling of retinal ganglion cells in Math5Cre-driven Knockout of Ezh2 and G9a in murine retinal ganglion cells at P0

Project description:Epigenetic mark deposition during embryonic development contribute to postnatal homeostasis and tissue stability. Previously, we found out that Ezh2 contributes critically to the function and postnatal cell survival in bipolar cells but not in retinal ganglion cells in the retina. (Yan et al. Postnatal onset of retinal degeneration by loss of embryonic Ezh2 repression of Six1. Sci Report. doi:10.1038/srep33887; (Cheng L, Wong LJ, Yan N, Han RC et al. Ezh2 does not mediate retinal ganglion cell homeostasis or their susceptibility to injury. PLoS One 2018;13(2):e0191853.). In this study, we used RNA-seq to define up- and down regulated genes in both Ezh2 and G9a deficient (Math5Cre; Ezh2f/fG9af/+; dKO) retinal ganglion cells (RGC) to evaluate the hypothesis of Ezh2 and G9a interaction that has been discussed in other tissues but in the retina. ChIP-Seq was applied to evaluate H3K27me3 histone marks in retinal ganglion cells in wild type (WT), Ezh2 (Math5Cre; Ezh2-/-, sKO) and combined G9a-Ezh2 (Math5Cre; G9a+/-Ezh2-/-, dKO) knockout mutant mice at P1.

2022-07-26 | GSE136360 | GEO

RNA-seq analysis of RNA from DBA/2J retinal ganglion cells

Project description:RNA-seq analysis from young and pre-glaucomatous DBA/2J retinal ganglion cells and control (age and sex-matched, D2-Gpnmb+) retinal ganglion cells

2017-03-07 | GSE90654 | GEO

Single-cell RNAseq data from mouse intrinsically-photosensitive retinal ganglion cells

Project description:The goal of this study was to characterize the transcriptomes of intrinsically-photosenstive retinal ganglion cells that innervate different retinorecipient target regions in the brain

2023-05-01 | GSE224600 | GEO

Transcription profiling of chicken whole eye and retinal ganglion cells (RGC) to identify RGC-specific genes

Project description:Identification of genes regulated by ATH5 in a dose-dependent manner.; Identification of retinal ganglion cells (RGC)-specific genes.

2009-01-14 | E-TABM-417 | biostudies-arrayexpress

Genetic signatures of retinal ganglion cells revealed through single cell profiling [non-dissociated RGC]

Project description:This study aimed to characterize the transcriptomes of adult retinal ganglion cells (RGCs). The physiological properties of these cells were characterized first in non-dissociated retinal tissue. With that information recorded, the cells were isolated for transcriptome profiling.

2019-11-01 | GSE115379 | GEO

Direct induction of retinal ganglion cell-like neurons

Project description:Retinal ganglion cells (RGCs) are the projection neurons in the retina that connect the visual sensing tissue to the brain. We found that Ascl1/Brn3b/Isl1 transcription factor combination can quickly and efficiently reprogramming mouse embryonic fibroblasts (MEFs) into retinal ganglion cell-like neurons (iRGCs). Using RNA-seq, we analyzed the transcriptomes of MEFs infected with Ascl1/Brn3b/Isl1-overexpressing viruses on day 2 or day 7 of reprogramming, or the final iRGCs on day 13 of reprogramming.

2020-09-30 | GSE140689 | GEO

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