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Enhanced polyhydroxyalkanoates production from Mixed Microbial Culture through Short Settling Aerobic Dynamic Feeding: improved selection and bulking resilience [ITS]

ABSTRACT: Enhanced polyhydroxyalkanoates production from Mixed Microbial Culture through Short Settling Aerobic Dynamic Feeding: improved selection and bulking resilience [ITS]

PROVIDER: PRJNA1295529 | ENA |

REPOSITORIES: ENA

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Enhanced polyhydroxyalkanoates production from Mixed Microbial Culture through Short Settling Aerobic Dynamic Feeding: improved selection and bulking resilience [18S]

Project description:This study explores the use of short settling times as a strategy to enhance microbial selection and prevent bulking induced by nutrient shortage in an aerobic dynamic feeding (ADF) process using mixed microbial cultures (MMCs) for polyhydroxyalkanoate (PHA) production from fermentation digestate. A 5.0-L aerobic reactor was operated under three conditions: Long Settling (LS, 30 minutes), Short Settling (SS, 10 minutes), and Short Settling under Nutrient Shortage (SS-NS, 10 minutes with reduced nutrient load). Short settling significantly improved biomass settleability, reducing the Sludge Volume Index (SVI) from 126 to 25 mL g⁻¹, and promoted the formation of dense flocs enriched in PHA-accumulating bacteria, as confirmed by transmission electron microscopy. Process kinetics revealed enhanced substrate uptake rates and improved storage yields under SS conditions, despite an elevated C/P ratio. Notably, nutrient shortage conditions were inadvertently reached by the proliferation of Polytoma mirum in the feeding tank. Although Polytoma mirum did not affect VFA composition, its presence significantly reduced nitrogen and phosphorus concentrations. These nutrient shortage conditions were maintained for at least 30 days until the system began to exhibit stress, as demonstrated by an increased SVI (178.6 mL g⁻¹). Microbial community analyses indicated marked shifts: the eukaryotic assemblage transitioned from sessile to motile ciliates under SS, while bacterial diversity within the PHA‐accumulating fraction remained high, with key taxa such as Sphaerotilus and Neomegalonema becoming more prevalent under phosphorus-limited conditions. Overall, short settling not only improved microbial selection but also prevented bulking by retaining well-aggregated biomass, thereby mitigating nutrient shortage conditions.

2025-07-24 | GSE303499 | GEO

Enhanced polyhydroxyalkanoates production from Mixed Microbial Culture through Short Settling Aerobic Dynamic Feeding: improved selection and bulking resilience [16S]

2025-07-24 | GSE303497 | GEO

Enhanced polyhydroxyalkanoates production from Mixed Microbial Culture through Short Settling Aerobic Dynamic Feeding: improved selection and bulking resilience [ITS]

2025-07-24 | GSE303500 | GEO

Enhanced polyhydroxyalkanoates production from Mixed Microbial Culture through Short Settling Aerobic Dynamic Feeding: improved selection and bulking resilience [16S]

Project description:Enhanced polyhydroxyalkanoates production from Mixed Microbial Culture through Short Settling Aerobic Dynamic Feeding: improved selection and bulking resilience [16S]

| PRJNA1295525 | ENA

Enhanced polyhydroxyalkanoates production from Mixed Microbial Culture through Short Settling Aerobic Dynamic Feeding: improved selection and bulking resilience [18S]

Project description:Enhanced polyhydroxyalkanoates production from Mixed Microbial Culture through Short Settling Aerobic Dynamic Feeding: improved selection and bulking resilience [18S]

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Settling and floating sludge

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Gene expression of thymic settling progenitors from embryos at E13 compared to E18

2013-09-17 | GSE50910 | GEO

Microbial community in oxic-settling-anoxic process

Project description:Microbial community in oxic-settling-anoxic process

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Gene expression of thymic settling progenitors from embryos at E13 compared to E18

Project description:We used whole genome transcriptome as gene discovery to further understand the differential behaviour of the first (embryonic) and second waves of thymic settling progenitors in the murine embryo. Mouse embryos at days 13 and 18 of gestation were isolated and the thymic lobes were dissected. Single cell suspension was prepared and treated with biotinilated antibodies to CD3, CD4, CD8, TER119, Gr1, CD25, CD19, NK1.1 and CD11c. Cell suspensions were then incubated with streptavidin Miltenyi Macs beads. Lineage negative thymocytes were recovered in Macs columns. Cell suspensions were then incubated with antibodies against CD135, CD117, CD44, CD24 and Streptavidin bound to PECy7. Lin-, CD117+, CD44+, CD24low, CD135+ cells were then isolated by cell sorting. 3 biological replicates of each sample were isolated with 104-3x104 cells

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Forest Resilience Initiative (FR)

Project description:Forest Resilience Initiative (FR)

| PRJNA1217485 | ENA