Project description:This SuperSeries is composed of the following subset Series: GSE21311: Maternal influences on the transmission of leukocyte gene expression profiles in population samples (Red Cross Donors) GSE21342: Maternal influences on the transmission of leukocyte gene expression profiles in population samples (mother and child) Refer to individual Series
Project description:This is a companion study to (GSE21342). Peripheral blood leukocyte samples were obtained with consent from 100 red cross blood donors sampled cross-sectionally across the city of Brisbane, Australia. After correction for RNA integrity values, individuals fall into major profiles of expression variation suggesting environmental and cultural influences on immune gene expression.
2011-01-01 | GSE21311 | GEO
Project description:Maternal influences on the transmission of leukocyte gene expression profiles in population samples
Project description:This is a companion study to (GSE21342). Peripheral blood leukocyte samples were obtained with consent from 100 red cross blood donors sampled cross-sectionally across the city of Brisbane, Australia. After correction for RNA integrity values, individuals fall into major profiles of expression variation suggesting environmental and cultural influences on immune gene expression. 100 individuals, 50 men and 50 women, were sampled with permission of the Red Cross Society of Australia and under informed consent. The age of individulas were between 18 and 68 (mean 44). BMI ranged from 17.7 to 51.3 with a mean of 26.6. Collection was carried out by EM at mobile Red Cross vans at 10 locations (Bellbowrie, Capalaba, Dayboro, Eagle Farm, Kenmore, Mount Ommaney, South Brisbane, St Lucia, Virginia, and Woolloongabba) distributed across the city.
Project description:Reticulocytes were purified from fresh blood samples obtained from 10 healthy adult volunteers (5 women and 5 men) after informed consent. The donors had normal blood cell counts, blood smears, hemoglobin electrophoresis, red cells membrane resistance tests and no biological evidence of hemolysis. Whole blood samples were centrifuged, the supernatant and the buffy coat containing white blood cells (WBC) and platelets were removed. The red cells pellet was then purified using the method described by Brun et al. Purity, assessed by Hematology Flow Cytometer was 1 leukocyte per 15 millions RBC and efficiency of purification process was 99.8% and 1 platelet per 10 000 RBC. Keywords: other
Project description:Each Whipple patient, infected patient and healthy individuals were arrayed against a pool of 3 blood donors from the Swiss Red Cross
