Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1298560 | ENA |
REPOSITORIES: ENA
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Project description:Background: Mobile phones have become ubiquitous. There are concerns about possible health hazards posed by mobile phone-based radio frequency electromagnetic field (RF-EMF). Such exposure may be recognized as external stressor at molecular level which can be identified up by shot gun proteomics approach. Objective: To study molecular effects of mobile phone-based RF-EMF exposure using shot gun proteomics. Methods: 40 individuals (30 mobile phone users and 10 non-users) were recruited based on the inclusion and exclusion criteria. Mobile phone users had mobile phone usage of more than a year, an age group of 18-65 years with no major medical or surgical illness and normal results of hemogram, liver function tests (LFT), kidney function tests (KFT) and random/fasting sugars. Non-users had no mobile phone, Wi-Fi, and microwave usage. Both mobile phone users and non-users were non-alcoholic and non-smoker. RF-EMF exposure from mobile phone and miscellaneous sources was assessed using questionnaire. Calling-time duration per day was used as a measure of mobile phone exposure and the users were classified accordingly. Five biological replicates (1 set with both genders, 2 sets each of males and females) of proteomics experiments were designed. Human plasma samples were immunodepleted of high abundant proteins, concentrated, reduced, alkylated, digested, and labelled by i-TRAQ reagents for mass spectrometry analysis. Bioinformatics study was done on differentially expressed proteins for gene ontology (GO) classification, Protein-protein interactions (PPIs) and pathway enrichment. Results: The study group was quite young (median age 24 years). Pathway enrichment studies found Dopamine mediated receptor signaling, Adrenaline and Noradrenalin synthesis, Plasminogen activating cascade, Cholecystokinin receptor (CCKR) signaling, Gonadotropin-releasing hormone (GnRH) pathway and Vitamin-D signaling and its metabolism to be implicated. Conclusion: The involvement of the differentially expressed proteins in various signalling cascades have implications physiologically albeit large sample size studies incorporating non-users need to be conducted to draw out clear inferences.
2026-06-07 | PXD036776 | Pride
Project description:Background: Mobile phones have become ubiquitous. There are concerns about possible health hazards posed by mobile phone-based radio frequency electromagnetic field (RF-EMF). Such exposure may be recognized as external stressor at molecular level which can be identified up by shot gun proteomics approach. Objective: To study molecular effects of mobile phone-based RF-EMF exposure using shot gun proteomics. Methods: 40 individuals (30 mobile phone users and 10 non-users) were recruited based on the inclusion and exclusion criteria. Mobile phone users had mobile phone usage of more than a year, an age group of 18-65 years with no major medical or surgical illness and normal results of hemogram, liver function tests (LFT), kidney function tests (KFT) and random/fasting sugars. Non-users had no mobile phone, Wi-Fi, and microwave usage. Both mobile phone users and non-users were non-alcoholic and non-smoker. RF-EMF exposure from mobile phone and miscellaneous sources was assessed using questionnaire. Calling-time duration per day was used as a measure of mobile phone exposure and the users were classified accordingly. Five biological replicates (1 set with both genders, 2 sets each of males and females) of proteomics experiments were designed. Human plasma samples were immunodepleted of high abundant proteins, concentrated, reduced, alkylated, digested, and labelled by i-TRAQ reagents for mass spectrometry analysis. Bioinformatics study was done on differentially expressed proteins for gene ontology (GO) classification, Protein-protein interactions (PPIs) and pathway enrichment. Results: The study group was quite young (median age 24 years). Pathway enrichment studies found Dopamine mediated receptor signaling, Adrenaline and Noradrenalin synthesis, Plasminogen activating cascade, Cholecystokinin receptor (CCKR) signaling, Gonadotropin-releasing hormone (GnRH) pathway and Vitamin-D signaling and its metabolism to be implicated. Conclusion: The involvement of the differentially expressed proteins in various signalling cascades have implications physiologically albeit large sample size studies incorporating non-users need to be conducted to draw out clear inferences.
2026-06-07 | PXD036802 | Pride
Project description:Background: Mobile phones have become ubiquitous. There are concerns about possible health hazards posed by mobile phone-based radio frequency electromagnetic field (RF-EMF). Such exposure may be recognized as external stressor at molecular level which can be identified up by shot gun proteomics approach. Objective: To study molecular effects of mobile phone-based RF-EMF exposure using shot gun proteomics. Methods: 40 individuals (30 mobile phone users and 10 non-users) were recruited based on the inclusion and exclusion criteria. Mobile phone users had mobile phone usage of more than a year, an age group of 18-65 years with no major medical or surgical illness and normal results of hemogram, liver function tests (LFT), kidney function tests (KFT) and random/fasting sugars. Non-users had no mobile phone, Wi-Fi, and microwave usage. Both mobile phone users and non-users were non-alcoholic and non-smoker. RF-EMF exposure from mobile phone and miscellaneous sources was assessed using questionnaire. Calling-time duration per day was used as a measure of mobile phone exposure and the users were classified accordingly. Five biological replicates (1 set with both genders, 2 sets each of males and females) of proteomics experiments were designed. Human plasma samples were immunodepleted of high abundant proteins, concentrated, reduced, alkylated, digested, and labelled by i-TRAQ reagents for mass spectrometry analysis. Bioinformatics study was done on differentially expressed proteins for gene ontology (GO) classification, Protein-protein interactions (PPIs) and pathway enrichment. Results: The study group was quite young (median age 24 years). Pathway enrichment studies found Dopamine mediated receptor signaling, Adrenaline and Noradrenalin synthesis, Plasminogen activating cascade, Cholecystokinin receptor (CCKR) signaling, Gonadotropin-releasing hormone (GnRH) pathway and Vitamin-D signaling and its metabolism to be implicated. Conclusion: The involvement of the differentially expressed proteins in various signalling cascades have implications physiologically albeit large sample size studies incorporating non-users need to be conducted to draw out clear inferences.
2026-06-07 | PXD036863 | Pride
Project description:Background: Mobile phones have become ubiquitous. There are concerns about possible health hazards posed by mobile phone-based radio frequency electromagnetic field (RF-EMF). Such exposure may be recognized as external stressor at molecular level which can be identified up by shot gun proteomics approach. Objective: To study molecular effects of mobile phone-based RF-EMF exposure using shot gun proteomics. Methods: 40 individuals (30 mobile phone users and 10 non-users) were recruited based on the inclusion and exclusion criteria. Mobile phone users had mobile phone usage of more than a year, an age group of 18-65 years with no major medical or surgical illness and normal results of hemogram, liver function tests (LFT), kidney function tests (KFT) and random/fasting sugars. Non-users had no mobile phone, Wi-Fi, and microwave usage. Both mobile phone users and non-users were non-alcoholic and non-smoker. RF-EMF exposure from mobile phone and miscellaneous sources was assessed using questionnaire. Calling-time duration per day was used as a measure of mobile phone exposure and the users were classified accordingly. Five biological replicates (1 set with both genders, 2 sets each of males and females) of proteomics experiments were designed. Human plasma samples were immunodepleted of high abundant proteins, concentrated, reduced, alkylated, digested, and labelled by i-TRAQ reagents for mass spectrometry analysis. Bioinformatics study was done on differentially expressed proteins for gene ontology (GO) classification, Protein-protein interactions (PPIs) and pathway enrichment. Results: The study group was quite young (median age 24 years). Pathway enrichment studies found Dopamine mediated receptor signaling, Adrenaline and Noradrenalin synthesis, Plasminogen activating cascade, Cholecystokinin receptor (CCKR) signaling, Gonadotropin-releasing hormone (GnRH) pathway and Vitamin-D signaling and its metabolism to be implicated. Conclusion: The involvement of the differentially expressed proteins in various signalling cascades have implications physiologically albeit large sample size studies incorporating non-users need to be conducted to draw out clear inferences.
2026-06-07 | PXD036866 | Pride
Project description:Background: Mobile phones have become ubiquitous. There are concerns about possible health hazards posed by mobile phone-based radio frequency electromagnetic field (RF-EMF). Such exposure may be recognized as external stressor at molecular level which can be identified up by shot gun proteomics approach. Objective: To study molecular effects of mobile phone-based RF-EMF exposure using shot gun proteomics. Methods: 40 individuals (30 mobile phone users and 10 non-users) were recruited based on the inclusion and exclusion criteria. Mobile phone users had mobile phone usage of more than a year, an age group of 18-65 years with no major medical or surgical illness and normal results of hemogram, liver function tests (LFT), kidney function tests (KFT) and random/fasting sugars. Non-users had no mobile phone, Wi-Fi, and microwave usage. Both mobile phone users and non-users were non-alcoholic and non-smoker. RF-EMF exposure from mobile phone and miscellaneous sources was assessed using questionnaire. Calling-time duration per day was used as a measure of mobile phone exposure and the users were classified accordingly. Five biological replicates (1 set with both genders, 2 sets each of males and females) of proteomics experiments were designed. Human plasma samples were immunodepleted of high abundant proteins, concentrated, reduced, alkylated, digested, and labelled by i-TRAQ reagents for mass spectrometry analysis. Bioinformatics study was done on differentially expressed proteins for gene ontology (GO) classification, Protein-protein interactions (PPIs) and pathway enrichment. Results: The study group was quite young (median age 24 years). Pathway enrichment studies found Dopamine mediated receptor signaling, Adrenaline and Noradrenalin synthesis, Plasminogen activating cascade, Cholecystokinin receptor (CCKR) signaling, Gonadotropin-releasing hormone (GnRH) pathway and Vitamin-D signaling and its metabolism to be implicated. Conclusion: The involvement of the differentially expressed proteins in various signalling cascades have implications physiologically albeit large sample size studies incorporating non-users need to be conducted to draw out clear inferences.
2026-06-07 | PXD036828 | Pride
Project description:Worldwide, number of mobile phone users have increased from 5.57 billion in 2011 to 6.8 billion in 2019. However short and long term impacts of the electromagnetic radiations emitting of mobile phone on tissue homeostasis with particular to brain proteome composition needs further investigation. In this study, we attempted a global proteome profiling study of rat hippocampus exposed to mobile phone radiation for 20 weeks (for 3 hrs/day for 5 days/week) to identify deregulated proteins and western blot analysis for validation. As a result, we identified 358 hippocampus proteins, of which 16 showed deregulation (log2 (exposed/control)>±1.0, p-value<0.05). Majority of these deregulated proteins grouped to three clusters sharing similar molecular functions/pathways. A set of four proteins (Aldehyde dehydrogenase:Aldh5a1, Na+ K+ transporting ATPase:Atp1b2, plasma membrane calcium transporting ATPase:PMCA and protein S100b) presenting each functional pathways were selected as important molecules. Western blot analysis of this protein set, expect Atp1b2, in independent samples corroborated the mass spectrometry findings. Aldh5a1 involve in cellular energy metabolism, both Atp1b2 and PMCA responsible for membrane transport and protein S100b has neuroprotective role. In conclusion, we present deregulated hippocampus proteome upon mobile phone radiation which might impact healthy functioning of brain.
2019-11-08 | MSV000084559 | MassIVE
Project description:Worldwide, number of mobile phone users have increased from 5.57 billion in 2011 to 6.8 billion in 2019. However short and long term impacts of the electromagnetic radiations emitting of mobile phone on tissue homeostasis with particular to brain proteome composition needs further investigation. In this study, we attempted a global proteome profiling study of rat hippocampus exposed to mobile phone radiation for 20 weeks (for 3 hrs/day for 5 days/week) to identify deregulated proteins and western blot analysis for validation. As a result, we identified 358 hippocampus proteins, of which 16 showed deregulation (log2 (exposed vs control). Majority of these deregulated proteins grouped to three clusters sharing similar molecular functions/pathways. A set of four proteins (Aldehyde dehydrogenase:Aldh5a1, Na+ K+ transporting ATPase:Atp1b2, plasma membrane calcium transporting ATPase:PMCA and protein S100b) presenting each functional pathways were selected as important molecules. Western blot analysis of this protein set, expect Atp1b2, in independent samples corroborated the mass spectrometry findings. Aldh5a1 involve in cellular energy metabolism, both Atp1b2 and PMCA responsible for membrane transport and protein S100b has neuroprotective role. In conclusion, we present deregulated hippocampus proteome upon mobile phone radiation which might impact healthy functioning of brain.
2019-12-20 | MSV000084732 | MassIVE
Project description:Worldwide, number of mobile phone users have increased from 5.57 billion in 2011 to 6.8 billion in 2019. However short and long term impacts of the electromagnetic radiations emitting of mobile phone on tissue homeostasis with particular to brain proteome composition needs further investigation. In this study, we attempted a global proteome profiling study of rat hippocampus exposed to mobile phone radiation for 20 weeks (for 3 hrs/day for 5 days/week) to identify deregulated proteins and western blot analysis for validation. As a result, we identified 358 hippocampus proteins, of which 16 showed deregulation (log2 (exposed/control), p-value<0.05). Majority of these deregulated proteins grouped to three clusters sharing similar molecular functions/pathways. A set of four proteins (Aldehyde dehydrogenase:Aldh5a1, Na+ K+ transporting ATPase:Atp1b2, plasma membrane calcium transporting ATPase:PMCA and protein S100b) presenting each functional pathways were selected as important molecules. Western blot analysis of this protein set, expect Atp1b2, in independent samples corroborated the mass spectrometry findings. Aldh5a1 involve in cellular energy metabolism, both Atp1b2 and PMCA responsible for membrane transport and protein S100b has neuroprotective role. In conclusion, we present deregulated hippocampus proteome upon mobile phone radiation which might impact healthy functioning of brain.
2019-12-20 | MSV000084731 | MassIVE